psPAX2慢病毒包装质粒

货号 名称 规格 优惠价
P0261 psPAX2慢病毒包装质粒

质粒干粉

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启动子: CAG promoter
复制子: pUC ori,SV40 ori
终止子: β-globin poly(A) signal,SV40 poly(A) signal
质粒分类: 病毒系列,慢病毒包装载体
原核抗性: 氨苄青霉素Amp
克隆菌株: 大肠杆菌Stbl3
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞293T
诱导方式: 无须诱导,瞬时表达
5'测序引物: pcaggs-F
3'测序引物: pcaggs-R
       2nd generation packaging vector. Known to work with many of the Aebischer and Trono lab lentiviral vectors, as well as the pLKO system. Produces higher titer than pCMV-dR8.2 dvpr.
LOCUS       Exported               10703 bp ds-DNA     circular SYN 02-MAY-2018
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..10703
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        4..383
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /note="chicken beta-actin promoter"
     intron          662..1670
                     /note="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             1780..3282
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /note="HIV-1 gag"
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             3075..6086
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /note="HIV-1 pol"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVGQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDGLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6660..6893
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     polyA_site      7792..7847
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     promoter        8379..8574
                     /note="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      8425..8560
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     polyA_signal    8580..8714
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(8953..9541)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9712..10572)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10573..10677)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgggccg gccgcgttga
     1741 cgcgcacggc aagaggcgag gggcggcgac tggtgagaga tgggtgcgag agcgtcagta
     1801 ttaagcgggg gagaattaga tcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     1861 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     1921 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     1981 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     2041 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     2101 caaaacaaaa gtaagaaaaa agcacagcaa gcagcagctg acacaggaca cagcaatcag
     2161 gtcagccaaa attaccctat agtgcagaac atccaggggc aaatggtaca tcaggccata
     2221 tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt cagcccagaa
     2281 gtgataccca tgttttcagc attatcagaa ggagccaccc cacaagattt aaacaccatg
     2341 ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac catcaatgag
     2401 gaagctgcag aatgggatag agtgcatcca gtgcatgcag ggcctattgc accaggccag
     2461 atgagagaac caaggggaag tgacatagca ggaactacta gtacccttca ggaacaaata
     2521 ggatggatga cacataatcc acctatccca gtaggagaaa tctataaaag atggataatc
     2581 ctgggattaa ataaaatagt aagaatgtat agccctacca gcattctgga cataagacaa
     2641 ggaccaaagg aaccctttag agactatgta gaccgattct ataaaactct aagagccgag
     2701 caagcttcac aagaggtaaa aaattggatg acagaaacct tgttggtcca aaatgcgaac
     2761 ccagattgta agactatttt aaaagcattg ggaccaggag cgacactaga agaaatgatg
     2821 acagcatgtc agggagtggg gggacccggc cataaagcaa gagttttggc tgaagcaatg
     2881 agccaagtaa caaatccagc taccataatg atacagaaag gcaattttag gaaccaaaga
     2941 aagactgtta agtgtttcaa ttgtggcaaa gaagggcaca tagccaaaaa ttgcagggcc
     3001 cctaggaaaa agggctgttg gaaatgtgga aaggaaggac accaaatgaa agattgtact
     3061 gagagacagg ctaatttttt agggaagatc tggccttccc acaagggaag gccagggaat
     3121 tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag gtttggggaa
     3181 gagacaacaa ctccctctca gaagcaggag ccgatagaca aggaactgta tcctttagct
     3241 tccctcagat cactctttgg cagcgacccc tcgtcacaat aaagataggg gggcaattaa
     3301 aggaagctct attagataca ggagcagatg atacagtatt agaagaaatg aatttgccag
     3361 gaagatggaa accaaaaatg atagggggaa ttggaggttt tatcaaagta ggacagtatg
     3421 atcagatact catagaaatc tgcggacata aagctatagg tacagtatta gtaggaccta
     3481 cacctgtcaa cataattgga agaaatctgt tgactcagat tggctgcact ttaaattttc
     3541 ccattagtcc tattgagact gtaccagtaa aattaaagcc aggaatggat ggcccaaaag
     3601 ttaaacaatg gccattgaca gaagaaaaaa taaaagcatt agtagaaatt tgtacagaaa
     3661 tggaaaagga aggaaaaatt tcaaaaattg ggcctgaaaa tccatacaat actccagtat
     3721 ttgccataaa gaaaaaagac agtactaaat ggagaaaatt agtagatttc agagaactta
     3781 ataagagaac tcaagatttc tgggaagttc aattaggaat accacatcct gcagggttaa
     3841 aacagaaaaa atcagtaaca gtactggatg tgggcgatgc atatttttca gttcccttag
     3901 ataaagactt caggaagtat actgcattta ccatacctag tataaacaat gagacaccag
     3961 ggattagata tcagtacaat gtgcttccac agggatggaa aggatcacca gcaatattcc
     4021 agtgtagcat gacaaaaatc ttagagcctt ttagaaaaca aaatccagac atagtcatct
     4081 atcaatacat ggatgatttg tatgtaggat ctgacttaga aatagggcag catagaacaa
     4141 aaatagagga actgagacaa catctgttga ggtggggatt taccacacca gacaaaaaac
     4201 atcagaaaga acctccattc ctttggatgg gttatgaact ccatcctgat aaatggacag
     4261 tacagcctat agtgctgcca gaaaaggaca gctggactgt caatgacata cagaaattag
     4321 tgggaaaatt gaattgggca agtcagattt atgcagggat taaagtaagg caattatgta
     4381 aacttcttag gggaaccaaa gcactaacag aagtagtacc actaacagaa gaagcagagc
     4441 tagaactggc agaaaacagg gagattctaa aagaaccggt acatggagtg tattatgacc
     4501 catcaaaaga cttaatagca gaaatacaga agcaggggca aggccaatgg acatatcaaa
     4561 tttatcaaga gccatttaaa aatctgaaaa caggaaaata tgcaagaatg aagggtgccc
     4621 acactaatga tgtgaaacaa ttaacagagg cagtacaaaa aatagccaca gaaagcatag
     4681 taatatgggg aaagactcct aaatttaaat tacccataca aaaggaaaca tgggaagcat
     4741 ggtggacaga gtattggcaa gccacctgga ttcctgagtg ggagtttgtc aatacccctc
     4801 ccttagtgaa gttatggtac cagttagaga aagaacccat aataggagca gaaactttct
     4861 atgtagatgg ggcagccaat agggaaacta aattaggaaa agcaggatat gtaactgaca
     4921 gaggaagaca aaaagttgtc cccctaacgg acacaacaaa tcagaagact gagttacaag
     4981 caattcatct agctttgcag gattcgggat tagaagtaaa catagtgaca gactcacaat
     5041 atgcattggg aatcattcaa gcacaaccag ataagagtga atcagagtta gtcagtcaaa
     5101 taatagagca gttaataaaa aaggaaaaag tctacctggc atgggtacca gcacacaaag
     5161 gaattggagg aaatgaacaa gtagatgggt tggtcagtgc tggaatcagg aaagtactat
     5221 ttttagatgg aatagataag gcccaagaag aacatgagaa atatcacagt aattggagag
     5281 caatggctag tgattttaac ctaccacctg tagtagcaaa agaaatagta gccagctgtg
     5341 ataaatgtca gctaaaaggg gaagccatgc atggacaagt agactgtagc ccaggaatat
     5401 ggcagctaga ttgtacacat ttagaaggaa aagttatctt ggtagcagtt catgtagcca
     5461 gtggatatat agaagcagaa gtaattccag cagagacagg gcaagaaaca gcatacttcc
     5521 tcttaaaatt agcaggaaga tggccagtaa aaacagtaca tacagacaat ggcagcaatt
     5581 tcaccagtac tacagttaag gccgcctgtt ggtgggcggg gatcaagcag gaatttggca
     5641 ttccctacaa tccccaaagt caaggagtaa tagaatctat gaataaagaa ttaaagaaaa
     5701 ttataggaca ggtaagagat caggctgaac atcttaagac agcagtacaa atggcagtat
     5761 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     5821 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     5881 aaaattttcg ggtttattac agggacagca gagatccagt ttggaaagga ccagcaaagc
     5941 tcctctggaa aggtgaaggg gcagtagtaa tacaagataa tagtgacata aaagtagtgc
     6001 caagaagaaa agcaaagatc atcagggatt atggaaaaca gatggcaggt gatgattgtg
     6061 tggcaagtag acaggatgag gattaacaca tggaattctg caacaactgc tgtttatcca
     6121 tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga gagcaagaaa
     6181 tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag cctaaaactg
     6241 cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt ttcatgacaa
     6301 aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga gctcatcaga
     6361 acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta atgcaaccta
     6421 taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt gtgtggtcca
     6481 tagtaatcat agaatatagg aaaatggccg ctgatcttca gacctggagg aggagatatg
     6541 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     6601 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     6661 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg
     6721 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     6781 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     6841 ctccaagcaa gaatcctagc tgtggaaaga tacctaaagg atcaacagct cctagggatt
     6901 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     6961 aataaatctc tggaacagat ctggaatcac acgacctgga tggagtggga cagagaaatt
     7021 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     7081 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     7141 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     7201 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     7261 tcgtttcaga cccacctccc aatcccgagg ggacccgaca ggcccgaagg aatagaagaa
     7321 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc cttggcactt
     7381 atctgggacg atctgcggag cctgtgcctc ttcagctacc accgcttgag agacttactc
     7441 ttgattgtaa cgaggattgt ggaacttctg ggacgcaggg ggtgggaagc cctcaaatat
     7501 tggtggaatc tcctacaata ttggagtcag gagctaaaga atagtgctgt tagcttgctc
     7561 aatgccacag ccatagcagt agctgagggg acagataggg ttatagaagt agtacaagga
     7621 gcttgtagag ctattcgcca catacctaga agaataagac agggcttgga aaggattttg
     7681 ctataagctc gaaacaaccg gtacctctag aactatagct agcagatctt tttccctctg
     7741 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     7801 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     7861 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     7921 atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa
     7981 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     8041 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     8101 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     8161 ttatgaagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     8221 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8281 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8341 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
     8401 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8461 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8521 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
     8581 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8641 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8701 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8761 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8821 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8881 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8941 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9001 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9061 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9121 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     9181 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9241 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9301 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9361 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9421 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9481 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9541 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9601 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9661 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9721 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9781 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9841 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9901 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9961 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10021 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10081 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10141 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10201 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10261 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10321 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10381 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10441 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10501 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10561 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10621 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10681 atttccccga aaagtgccac ctg
//