pENTR223-DUSP6人源基因克隆质粒

货号 名称 规格 优惠价
P0625 pENTR223-DUSP6人源基因克隆质粒

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别名: NM_001946.3,HH19; MKP3; PYST1
原核抗性: 壮观霉素Spe
克隆菌株: 大肠杆菌DH5α
培养条件: 37℃
           pENTR223-DUSP6(NM_001946.3) 是一个人源cDNA基因克隆质粒。 Homo sapiens dual specificity phosphatase 6 (DUSP6), transcript variant 1, mRNA.
LOCUS       Exported                3933 bp ds-DNA     circular SYN 16-JAN-2018
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      88..115
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      207..293
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     357..373
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    389..487
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    488..1630
                     /label=DUSP6
     protein_bind    complement(1632..1731)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1749..1767)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1772..1788)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2219..3010
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=Spe
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3103..3691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
       61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
      481 agttggcatg atagatacgc tcagacccgt gcccttcgcg tcggaaatgg cgatcagcaa
      541 gacggtggcg tggctcaacg agcagctgga gctgggcaac gagcggctgc tgctgatgga
      601 ctgccggccg caggagctat acgagtcgtc gcacatcgag tcggccatca acgtggccat
      661 cccgggcatc atgctgcggc gcctgcagaa gggtaacctg ccggtgcgcg cgctcttcac
      721 gcgcggcgag gaccgggacc gcttcacccg gcgctgtggc accgacacag tggtgctcta
      781 cgacgagagc agcagcgact ggaacgagaa tacgggcggc gagtcggtgc tcgggctgct
      841 gctcaagaag ctcaaggacg agggctgccg ggcgttctac ctggaaggtg gcttcagtaa
      901 gttccaagcc gagttctccc tgcattgcga gaccaatcta gacggctcgt gtagcagcag
      961 ctcgccgccg ttgccagtgc tggggctcgg gggcctgcgg atcagctctg actcttcctc
     1021 ggacatcgag tctgaccttg accgagaccc caatagtgca acagactcgg atggtagtcc
     1081 gctgtccaac agccagcctt ccttcccagt ggagatcttg cccttcctct acttgggctg
     1141 tgccaaagac tccaccaact tggacgtgtt ggaggaattc ggcatcaagt acatcttgaa
     1201 cgtcaccccc aatttgccga atctctttga gaacgcagga gagtttaaat acaagcaaat
     1261 ccccatctcg gatcactgga gccaaaacct gtcccagttt ttccctgagg ccatttcttt
     1321 catagatgaa gcccggggca agaactgtgg tgtcttggta cattgcttgg ctggcattag
     1381 ccgctcagtc actgtgactg tggcttacct tatgcagaag ctcaatctgt cgatgaacga
     1441 tgcctatgac attgtcaaaa tgaaaaaatc caacatatcc cctaacttca acttcatggg
     1501 tcagctgctg gacttcgaga ggacgctggg actcagcagc ccatgtgaca acagggttcc
     1561 agcacagcag ctgtatttta ccaccccttc caaccagaat gtataccagg tggactctct
     1621 gcaatctacg tgcccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     1681 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     1741 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     1801 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct
     1861 ctagaccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg
     1921 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     1981 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     2041 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     2101 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     2161 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     2221 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     2281 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     2341 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     2401 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     2461 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     2521 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     2581 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     2641 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     2701 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     2761 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     2821 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     2881 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     2941 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     3001 cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3061 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3121 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3181 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3241 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3301 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3361 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3421 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3481 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3541 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3601 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3661 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3721 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3781 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3841 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     3901 cgcgcgttgg ccgattcatt aatgcagctg gca
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