名称: | pFC330 |
---|---|
别称: | Plasmid #87842 |
基因长: | |
质粒宿主: | 丝状真菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | pyrG |
荧光标记: | |
启动子: | tef1 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GCGCTGCGAT
GATACCGCGA
GAACCACGCT
CACCGGCTCC
GGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
CGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCGCTACAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
TTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTCA
GTGTTACAAC
CAATTAACCA
ATTCTGAACA
TTATCGCGAG
CCCATTTATA
CCTGAATATG
GCTCATAACA
CCCCTTGTTT
GCCTGGCGGC
AGTAGCGCGG
TGGTCCCACC
TGACCCCATG
CCGAACTCAG
AAGTGAAACG
CCGTAGCGCC
GATGGTAGTG
TGGGGACTCC
CCATGCGAGA
GTAGGGAACT
GCCAGGCATC
AAATAAAACG
AAAGGCTCAG
TCGAAAGACT
GGGCCTTTCG
CCCGGGCTAA
TTATGGGGTG
TCGCCCTTAT
TCGACTCTAT
AGTGAAGTTC
CTATTCTCTA
GAAAGTATAG
GAACTTCTGA
AGTGGGGATT
TAAATGCGGC
CGCGCTGAGG
GTTTAATCGA
CGAAGCAGCT
GACGGCCAGT
GCCAAGCTTA
ACGCGTACCG
GGCCCAGTAT
ATGTTCCGCA
GATGACTGGA
GCTCTGCCAT
ACGTGCCCTC
TCAAGCACCA
TTTGTTCCAT
CTACAGAGAC
TAGTCACCAA
CTAGTCTATC
AAGACTCACA
GGGTACATTG
CTGAGACCAA
CTGACCAGAG
GCAGGGTAGC
GGATTGACGG
CTCCATCTCC
TTCACTTACA
AGGTCTATTG
AAAGCCCTTT
AGCATCACCA
AGCGGAGAAT
AGATTGTTAA
GCTTATTTTT
TGTATACTGT
TTTGTGATAG
CACGAAGTTT
TTCCACGGTA
TCTTGTTAAA
AATATATATT
TGTGGCGGGC
TTACCTACAT
CAAATTAATA
AGAGACTAAT
TATAAACTAA
ACACACAAGC
AAGCTACTTT
AGGGTAAAAG
TTTATAAATG
CTTTTGACGT
ATAAACGTTG
CTTGTATTTA
TTATTACAAT
TAAAGGTGGA
TAGAAAACCT
AGAGACTAGT
TAGAAACTAA
TCTCAGGTTT
GCGTTAAACT
AAATCAGAGC
CCGAGAGGTT
AACAGAACCT
AGAAGGGGAC
TAGATATCCG
GGTAGGGAAA
CAAAAAAAAA
AANCAAGACA
GCCACATATT
AGGGAGACTA
GTTAGAAGCT
AGTTCCAGGA
CTAGGAAAAT
AAAAGACAAT
GATACCACAG
TCTAGTTGAC
AACTAGATAG
ATTCTAGATT
GAGGCCAAAG
TCTCTGAGAT
CCAGGTTAGT
TGCAACTAAT
ACTAGTTAGT
ATCTAGTCTC
CTATAACTCT
GAAGCTAGAA
TAACTTACTA
CTATTATCCT
CACCACTGTT
CAGCTGCGCA
AACGGAGTGA
TTGCAAGGTG
TTCAGAGACT
AGTTATTGAC
TAGTCAGTGA
CTAGCAATAA
CTAACAAGGT
ATTAACCTAC
CATGTCTGCC
ATCACCCTGC
ACTTCCTCGG
GCTCAGCAGC
CTTTTCCTCC
TCATTTTCAT
GCTCATTTTC
CTTGTTTAAG
ACTGTGACTA
GTCRAAGACT
AGTCCAGAAC
CACAAAGGAG
AAATGTCTTA
CCACTTTCTT
CATTGCTTGT
CTCTTTTGCA
TTATCCATGT
CTGCAACTAG
TTAGAGTCTA
GTTAGTGACT
AGTCCGACGA
GGACTTGCTT
GTCTCCGGAT
TGTTGGAGGA
ACTCTCCAGG
GCCTCAAGAT
CCACAACAGA
GCCTTCTAGA
AGACTGGTCA
ATAACTAGTT
GGTCTTTGTC
TGAGTCTGAC
TTACGAGGTT
GCATACTCGC
TCCCTTTGCC
TCGTCAATCG
ATGAGAAAAA
GCGCCAAAAC
TCGCAATATG
GCTTTGAACC
ACACGGTGCT
GAGACTAGTT
AGAATCTAGT
CCCAAACTAG
CTTGGATAGC
TTACCTTTGC
CCTTTGCGTT
GCGACAGGTC
TTGCAGGGTA
TGGTTCCTTT
CTCACCAGCT
GATTTAGCTG
CCTTGCTACC
CTCACGGCGG
ATCTGCATAA
AGAGTGGCTA
GAGGTTATAA
ATTAGCACTG
ATCCTAGGTA
CGGGGCTGAA
TGTAACTTGC
CYTTCCTTTC
TCATCGCGCG
GCAAGACAGG
CTTGCTCAAA
TTCCTACCAG
TCACAGGGGT
ATGCACGGCG
TACGGACCAC
TTGAACTAGT
CACAGATTAG
TTAGCAACTA
GTCTGCATTG
AATGGCTGTA
CTTACGGGCC
CTCGCCATTG
TCCTGATCAT
TTCCAGCTTC
ACCCTCGTTG
CTGCAAAGTA
GTTAGTGACT
AGTCAAGGAC
TAGTTGAAAT
GGGAGAAGAA
ACTCACGAAT
TCTCGACACC
CTTAGTATTG
TGGTCCTTGG
ACTTGGTGCT
GCTATATATT
AGCTAATACA
CTAGTTAGAC
TCACRGAAAC
TTACGCAGCT
CGCTTGCGCT
TCTTGGTAGG
AGTCGGGGTT
GGGAGAACAG
TGCCTTCAAA
CAAGCCTTCA
TACCATGCTA
CTTGACTAGT
CAGGGACTAG
TCACCAAGTA
ATCTAGATAG
GACTTGCCTT
TGGCCTCCAT
CAGTTCCTTC
ATAGTGGGAG
GTCCATTGTG
CAATGTAAAC
TCCATGCCGT
GGGAGTTCTT
GTCCTTCAAG
TGCTTGACCA
ATATGTTTCT
GTTGGCAGAG
GGAACCTGTC
AACTAGTTAA
TAACTAGTCA
GAAACTAGTA
TAGCAGTAGA
CTCACTGTAC
GCTTGAGGCA
TCCCTTCACT
CGGCAGTAGA
CTTCATATGG
ATGGATATCA
GGCACGCCAT
TGTCGTCCTG
TGGACTAGTC
AGTAACTAGG
CTTAAAGCTA
GTCGGGTCGG
CTTACTATCT
TGAAATCCGG
CAGCGTAAGC
TCCCCGTCCT
TAACTGCCTC
GAGATAGTGA
CAGTACTCTG
GGGACTTTCG
GAGATCGTTA
TCGCGAATGC
TCGGCATACT
AATCGTTGAC
TAGTCTTGGA
CTAGTCCCGA
GCAAAAAGGA
TTGGAGGAGG
AGGAGGAAGG
TGAGAGTGAG
ACAAAGAGCG
AAATAAGAGC
TTCAAAGGCT
ATCTCTAAGC
AGTATGAAGG
TTAAGTATCT
AGTTCTTGAC
TAGATTTAAA
AGAGATTTCG
ACTAGTTATG
TACCTGGAGT
TTGGATATAG
GAATGTGTTG
TGGTAACGAA
ATGTAAGGGG
GAGGAAAGAA
AAAGTCGGTC
AAGAGGTAAC
TCTAAGTCGG
CCATTCCTTT
TTGGGAGGCG
CTAACCATAA
ACGGCATGGT
CGACTTAGAG
TTAGCTCAGG
GAATTTAGGG
AGTTATCTGC
GACCACCGAG
GAACGGCGGA
ATGCCAAAGA
ATCCCGATGG
AGCTCTAGCT
GGCGGTTGAC
AACCCCACCT
TTTGGCGTTT
CTGCGGCGTT
GCAGGCGGGA
CTGGATACTT
CGTAGAACCA
GAAAGGCAAG
GCAGAACGCG
CTCAGCAAGA
GTGTTGGAAG
TGATAGCATG
ATGTGCCTTG
TTAACTAGGT
CAAAATCTGC
AGTATGCTTG
ATGTTATCCA
AAGTGTGAGA
GAGGAAGGTC
CAAACATACA
CGATTGGGAG
AGGGCCTAGG
TATAAGAGTT
TTTGAGTAGA
ACGCATGTGA
GCCCAGCCAT
CTCGAGGAGA
TTAAACACGG
GCCGGCATTT
GATGGCTATG
TTAGTACCCC
AATGGAAACG
GTGAGAGTCC
AGTGGTCGCA
GATAACTCCC
TAAATTCCCT
GAGCTAACTC
TAAGTCGACC
ATGCCGTTTA
TGGTTAGCGC
CTCCCAAAAA
GGAATGGCCG
ACTTAGAGTT
ACCTCTTGAC
CGACTTTTTC
TTTCCTCCCC
CTTACATTTC
GTTACCACAA
CACATTCCTA
TATCCAAACT
CCAGGTACAT
AACTAGTCGA
AATCTCTTTT
AAATCTAGTC
AAGAACTAGA
TACTTAACCT
TCATACTGCT
TAGAGATAGC
CTTTGAAGCT
CTTATTTCGC
TCTTTGTCTC
ACTCTCACCT
TCCTCCTCCT
CCTCCAATCC
TTTTTGCTCG
GGACTAGTCC
AAGACTAGTC
AACGATTAGT
ATGCCGAGCA
TTCGCGATAA
CGATCTCCGA
AAGTCCCCAG
AGTACTGTCA
CTATCTCGAG
GCAGTTAAGG
ACGGGGAGCT
TACGCTGCCG
GATTTCAAGA
TAGTAAGCCG
ACCCGACTAG
CTTTAAGCCT
AGTTACTGAC
TAGTCCACAG
GACGACAATG
GCGTGCCTGA
TATCCATCCA
TATGAAGTCT
ACTGCCGAGT
GAAGGGATGC
CTCAAGCGTA
CAGTGAGTCT
ACTGCTATAC
TAGTTTCTGA
CTAGTTATTA
ACTAGTTGAC
AGGTTCCCTC
TGCCAACAGA
AACATATTGG
TCAAGCACTT
GAAGGACAAG
AACTCCCACG
GCATGGAGTT
TACATTGCAC
AATGGACCTC
CCACTATGAA
GGAACTGATG
GAGGCCAAAG
GCAAGTCCTA
TCTAGATTAC
TTGGTGACTA
GTCCCTGACT
AGTCAAGTAG
CATGGTATGA
AGGCTTGTTT
GAAGGCACTG
TTCTCCCAAC
CCCGACTCCT
ACCAAGAAGC
GCAAGCGAGC
TGCGTAAGTT
TCYGTGAGTC
TAACTAGTGT
ATTAGCTAAT
ATATAGCAGC
ACCAAGTCCA
AGGACCACAA
TACTAAGGGT
GTCGAGAATT
CGTGAGTTTC
TTCTCCCATT
TCAACTAGTC
CTTGACTAGT
CACTAACTAC
TTTGCAGCAA
CGAGGGTGAA
GCTGGAAATG
ATCAGGACAA
TGGCGAGGGC
CCGTAAGTAC
AGCCATTCAA
TGCAGACTAG
TTGCTAACTA
ATCTGTGACT
AGTTCAAGTG
GTCCGTACGC
CGTGCATACC
CCTGTGACTG
GTAGGAATTT
GAGCAAGCCT
GTCTTGCCGC
GCGATGAGAA
AGGAARGGCA
AGTTACATTC
AGCCCCGTAC
CTAGGATCAG
TGCTAATTTA
TAACCTCTAG
CCACTCTTTA
TGCAGATCCG
CCGTGAGGGT
AGCAAGGCAG
CTAAATCAGC
TGGTGAGAAA
GGAACCATAC
CCTGCAAGAC
CTGTCGCAAC
GCAAAGGGCA
AAGGTAAGCT
ATCCAAGCTA
GTTTGGGACT
AGATTCTAAC
TAGTCTCAGC
ACCGTGTGGT
TCAAAGCCAT
ATTGCGAGTT
TTGGCGCTTT
TTCTCATCGA
TTGACGAGGC
AAAGGGAGCG
AGTATGCAAC
CTCGTAAGTC
AGACTCAGAC
AAAGACCAAC
TAGTTATTGA
CCAGTCTTCT
AGAAGGCTCT
GTTGTGGATC
TTGAGGCCCT
GGAGAGTTCC
TCCAACAATC
CGGAGACAAG
CAAGTCCTCG
TCGGACTAGT
CACTAACTAG
ACTCTAACTA
GTTGCAGACA
TGGATAATGC
AAAAGAGACA
AGCAATGAAG
AAAGTGGTAA
GACATTTCTC
CTTTGTGGTT
CTGGACTAGT
CTTYGACTAG
TCACAGTCTT
AAACAAGGAA
AATGAGCATG
AAAATGAGGA
GGAAAAGGCT
GCTGAGCCCG
AGGAAGTGCA
GGGTGATGGC
AGACATGGTA
GGTTAATACC
TTGTTAGTTA
TTGCTAGTCA
CTGACTAGTC
AATAACTAGT
CTCTGAACAC
CTTGCAATCA
CTCCGTTTGC
GCAGCTGAAC
AGTGGTGAGG
ATAATAGTAG
TAAGTTATTC
TAGCTTCAGA
GTTATAGGAG
ACTAGATACT
AACTAGTATT
AGTTGCAACT
AACCTGGATC
TCAGAGACTT
TGGCCTCAAT
CTAGAATCTA
TCTAGTTGTC
AACTAGACTG
TGGTATCATT
GTCTTTTATT
TTCCTAGTCC
TGGAACTAGC
TTCTAACTAG
TCTCCCTAAT
ATGTGGCTGT
CTTGTTTTTT
TTTTTTGTTT
CCCTACCCGG
ATATCTAGTC
CCCTTCTAGG
TTCTGTTAAC
CTCTCGGGCT
CTGATTTAGT
TTAACGCAAA
CCTGAGATTA
GTTTCTAACT
AGTCTCTAGG
TTTTCTATCC
ACCTTTAATT
GTAATAATAA
ATACAAGCAA
CGTTTATACG
TCAAAAGCAT
TTATAAACTT
TTACCCTAAA
GTAGCTTGCT
TGTGTGTTTA
GTTTATAATT
AGTCTCTTAT
TAATTTGATG
TAGGTAAGCC
CGCCACAAAT
ATATATTTTT
AACAAGATAC
CGTGGAAAAA
CTTCGTGCTA
TCACAAAACA
GTATACAAAA
AATAAGCTTA
ACAATCTATT
CTCCGCTTGG
TGATGCTAAA
GGGCTTTCAA
TAGACCTTGT
AAGTGAAGGA
GATGGAGCCG
TCAATCCGCT
ACCCTGCCTC
TGGTCAGTTG
GTCTCAGCAA
TGTACCCTGT
GAGTCTTGAT
AGACTAGTTG
GTGACTAGTC
TCTGTAGATG
GAACAAATGG
TGCTTGAGAG
GGCACGTATG
GCAGAGCTCC
AGTCATCTGC
GGAACATATA
CTGGGCCCGG
GAAGATCTCA
TGGTCATAGC
TGTTTCCGCT
GAGGGTTTAA
TTAAGACCTC
AGCCGAGACA
GCAGAATCAC
CGCCCAAGTT
AAGCCTTTGT
GCTGATCATG
CTCTCGAACG
GGCCAAGTTC
GGGAAAAGCA
AAGGAGCGTT
TAGTGAGGGG
CAATTTGACT
CACCTCCCAG
GCAACAGATG
AGGGGGGCAA
AAAGAAAGAA
ATTTTCGTGA
GTCAATATGG
ATTCCGAGCA
TCATTTTCTT
GCGGTCTATC
TTGCTACGTA
TGTTGATCTT
GACGCTGTGG
ATCAAGCAAC
GCCACTCGCT
CGCTCCATCG
CAGGCTGGTC
GCAGACAAAT
TAAAAGGCGG
CAAACTCGTA
CAGCCGCGGG
GTTGTCCGCT
GCAAAGTACA
GAGTGATAAA
AGCCGCCATG
CGACCATCAA
CGCGTTGATG
CCCAGCTTTT
TCGATCCGAG
AATCCACCGT
AGAGGCGATA
GCAAGTAAAG
AAAAGCTAAA
CAAAAAAAAA
TTTCTGCCCC
TAAGCCATGA
AAACGAGATG
GGGTGGAGCA
GAACCAAGGA
AAGAGTCGCG
CTGGGCTGCC
GTTCCGGAAG
GTGTTGTAAA
GGCTCGACGC
CCAAGGTGGG
AGTCTAGGAG
AAGAATTTGC
ATCGGGAGTG
GGGCGGGTTA
CCCCTCCATA
TCCAATGACA
GATATCTACC
AGCCAAGGGT
TTGAGCCCGC
CCGCTTAGTC
GTCGTCCTCG
CTTGCCCCTC
CATAAAAGGA
TTTCCCCTCC
CCCTCCCACA
AAATTTTCTT
TCCCTTCCTC
TCCTTGTCCG
CTTCAGTACG
TATATCTTCC
CTTCCCTCGC
TTCTCTCCTC
CATCCTTCTT
TCATCCATCT
CCTGCTAACT
TCTCTGCTCA
GCACCTCTAC
GCATTACTAG
CCGTAGTATC
TGAGCACTTC
TCCCTTTTAT
ATTCCACAAA
ACATAACACA
ACCTTCACCA
TGGACAAGAA
GTATAGCATC
GGGCTGGACA
TTGGAACGAA
CTCGGTTGGT
TGGGCTGTGA
TTACGGACGA
ATACAAGGTG
CCATCCAAGA
AGTTTAAGGT
CCTGGGAAAC
ACCGACCGTC
ACTCAATCAA
GAAGAATCTC
ATTGGAGCCC
TGCTCTTCGA
TAGTGGGGAG
ACCGCCGAAG
CTACTCGACT
GAAGCGAACG
GCTCGCCGGC
GTTATACACG
ACGCAAGAAT
CGCATCTGCT
ACCTCCAGGA
GATTTTCAGC
AACGAAATGG
CTAAGGTTGA
TGACTCATTC
TTTCATCGAC
TCGAAGAAAG
TTTCTTGGTC
GAGGAGGATA
AGAAGCACGA
GCGCCATCCG
ATCTTTGGTA
ACATTGTGGA
TGAGGTTGCC
TATCACGAAA
AGTACCCAAC
TATCTATCAT
CTTCGTAAGA
AGCTGGTCGA
TAGCACGGAC
AAGGCTGATT
TGCGACTTAT
CTACCTGGCA
CTCGCGCACA
TGATTAAGTT
CCGCGGCCAT
TTTCTTATCG
AGGGTGACCT
GAACCCCGAT
AATTCTGACG
TTGATAAGCT
CTTCATCCAG
TTGGTCCAAA
CCTACAATCA
GCTGTTTGAG
GAAAACCCTA
TTAATGCATC
TGGCGTGGAC
GCCAAGGCTA
TCCTTTCGGC
GCGCCTGTCT
AAGTCGCGGC
GTTTGGAGAA
CCTTATCGCA
CAACTCCCCG
GCGAAAAGAA
GAACGGCCTC
TTCGGTAATT
TGATTGCGTT
GTCACTTGGT
CTGACTCCTA
ACTTCAAGAG
TAATTTTGAC
CTGGCAGAGG
ATGCGAAGCT
CCAGTTGTCT
AAGGATACGT
ATGATGACGA
TCTCGACAAC
TTGCTTGCCC
AAATCGGTGA
CCAGTACGCT
GATCTTTTCC
TGGCCGCTAA
GAATCTCTCA
GATGCAATCC
TGCTCAGTGA
CATTTTGCGG
GTCAACACCG
AGATTACTAA
GGCCCCCCTG
TCAGCTAGTA
TGATCAAGCG
GTATGATGAG
CACCATCAGG
ACCTCACCTT
GCTTAAGGCC
CTCGTGCGTC
AGCAATTGCC
TGAGAAGTAT
AAGGAAATCT
TCTTTGACCA
ATCCAAGAAC
GGATACGCAG
GGTATATTGA
TGGCGGTGCG
AGCCAGGAGG
AATTCTACAA
GTTTATCAAG
CCGATTTTGG
AGAAGATGGA
CGGCACTGAG
GAACTGCTCG
TCAAGCTGAA
TCGCGAAGAT
TTGCTTCGTA
AGCAACGAAC
GTTCGACAAC
GGCTCCATCC
CGCACCAGAT
TCATCTGGGC
GAGCTCCACG
CCATCCTTCG
ACGCCAGGAA
GATTTCTACC
CATTTCTGAA
GGACAACCGT
GAGAAGATCG
AAAAGATTCT
TACATTCCGA
ATCCCCTACT
ATGTGGGACC
TTTGGCCCGT
GGGAATTCCC
GATTTGCTTG
GATGACCCGA
AAGAGCGAGG
AAACCATCAC
TCCGTGGAAC
TTCGAGGAAG
TCGTGGACAA
GGGTGCATCC
GCGCAGAGCT
TCATTGAGCG
GATGACCAAT
TTTGATAAGA
ACCTTCCGAA
TGAAAAGGTC
CTGCCAAAGC
ATTCGCTGCT
CTACGAGTAT
TTCACCGTGT
ATAACGAACT
GACTAAGGTC
AAGTACGTGA
CGGAGGGAAT
GCGGAAGCCA
GCCTTCCTCT
CAGGGGAACA
AAAGAAGGCT
ATCGTCGATT
TGCTTTTTAA
GACCAATCGT
AAAGTGACTG
TTAAGCAGCT
GAAGGAGGAT
TATTTCAAGA
AGATTGAATG
TTTCGACTCC
GTCGAGATCA
GCGGCGTGGA
AGATCGCTTT
AACGCTTCCC
TCGGTACCTA
CCACGACCTG
CTCAAGATCA
TTAAGGACAA
GGATTTCCTC
GATAACGAGG
AAAATGAGGA
CATCTTGGAA
GATATTGTCC
TCACGTTGAC
ACTTTTTGAG
GACCGCGAAA
TGATCGAGGA
ACGGCTCAAG
ACATATGCCC
ATTTGTTCGA
CGATAAGGTG
ATGAAGCAGC
TGAAGCGGCG
TCGATACACC
GGATGGGGTC
GCCTTAGCCG
GAAGCTGATC
AACGGCATTC
GAGATAAGCA
ATCTGGTAAG
ACTATCTTGG
ATTTCCTTAA
GTCGGACGGC
TTCGCCAACC
GCAATTTTAT
GCAGCTTATT
CACGACGATT
CCCTGACGTT
CAAGGAGGAC
ATCCAGAAGG
CACAAGTCTC
AGGACAAGGG
GATTCCCTGC
ACGAGCATAT
CGCCAACCTG
GCTGGATCCC
CGGCGATCAA
GAAGGGGATT
CTTCAGACCG
TCAAGGTTGT
CGACGAGCTG
GTCAAGGTGA
TGGGCCGTCA
TAAGCCAGAA
AACATCGTGA
TTGAGATGGC
CCGAGAAAAT
CAGACCACTC
AAAAGGGTCA
GAAGAACAGC
CGCGAGCGGA
TGAAGCGGAT
CGAGGAAGGC
ATTAAGGAAC
TTGGTTCTCA
GATCCTGAAG
GAGCACCCTG
TTGAAAACAC
ACAGCTCCAA
AATGAGAAGC
TGTATCTCTA
CTATTTGCAA
AATGGACGCG
ACATGTACGT
CGATCAGGAG
CTCGACATTA
ACCGGTTGTC
GGACTACGAT
GTTGACCATA
TCGTCCCGCA
ATCCTTCCTT
AAGGACGATA
GCATTGATAA
CAAGGTGCTG
ACTCGCTCAG
ATAAGAACCG
GGGCAAGTCC
GACAATGTTC
CAAGCGAGGA
AGTGGTTAAG
AAGATGAAGA
ACTACTGGCG
CCAATTGCTT
AATGCCAAGC
TCATCACACA
GCGCAAGTTT
GACAACTTGA
CCAAGGCCGA
GCGGGGAGGG
CTGAGTGAAC
TCGATAAGGC
TGGCTTCATC
AAGCGTCAAC
TCGTGGAGAC
GCGACAGATC
ACAAAGCACG
TTGCTCAGAT
TCTGGACTCC
CGGATGAACA
CAAAGTACGA
CGAGAATGAT
AAGCTCATCC
GTGAAGTTAA
GGTCATTACC
CTCAAGTCTA
AGTTGGTGTC
GGATTTCCGC
AAGGACTTCC
AATTTTATAA
GGTTCGGGAG
ATCAACAATT
ATCACCATGC
ACATGATGCG
TACCTCAACG
CAGTCGTGGG
AACTGCGCTC
ATCAAGAAGT
ATCCCAAGTT
GGAGTCCGAA
TTCGTCTACG
GGGATTATAA
GGTTTACGAC
GTCCGCAAGA
TGATCGCCAA
GAGTGAGCAG
GAAATTGGCA
AGGCCACGGC
TAAGTATTTC
TTTTACTCCA
ACATCATGAA
TTTCTTTAAG
ACGGAGATCA
CACTCGCCAA
TGGAGAAATC
CGTAAGCGAC
CTTTGATTGA
GACCAACGGC
GAGACTGGTG
AAATCGTTTG
GGATAAGGGG
CGCGACTTCG
CTACCGTGCG
GAAGGTTCTG
AGCATGCCGC
AAGTCAATAT
CGTCAAGAAA
ACCGAGGTGC
AGACAGGCGG
TTTCTCTAAG
GAATCGATTC
TTCCAAAGCG
TAACTCTGAC
AAGCTGATCG
CTCGAAAGAA
GGATTGGGAC
CCCAAGAAGT
ATGGAGGGTT
CGATTCTCCT
ACAGTGGCAT
ACTCGGTTCT
CGTTGTCGCG
AAGGTTGAGA
AGGGAAAGTC
TAAGAAGCTG
AAGTCGGTCA
AGGAACTGCT
CGGGATCACC
ATTATGGAGC
GCTCCAGCTT
CGAAAAGAAT
CCCATCGACT
TTCTCGAGGC
CAAGGGCTAT
AAGGAAGTCA
AGAAGGATCT
TATCATTAAG
CTGCCTAAGT
ACTCTTTGTT
CGAGCTTGAA
AACGGTCGAA
AGCGAATGCT
CGCATCGGCA
GGAGAGTTGC
AGAAGGGGAA
TGAATTGGCA
CTTCCCTCAA
AGTACGTGAA
CTTCCTGTAT
CTCGCGTCCC
ACTACGAGAA
GCTGAAGGGT
AGCCCTGAGG
ACAACGAACA
GAAGCAACTT
TTTGTTGAGC
AACACAAGCA
TTATCTGGAT
GAGATCATTG
AACAGATTTC
AGAGTTCAGT
AAGCGCGTCA
TCCTCGCCGA
TGCTAATCTC
GACAAGGTGT
TGTCGGCCTA
CAACAAGCAC
CGTGACAAGC
CGATCCGAGA
GCAGGCTGAA
AATATCATTC
ATCTGTTCAC
CCTCACTAAC
TTGGGAGCAC
CAGCAGCGTT
CAAGTATTTT
GATACGACAA
TCGACCGTAA
GCGATACACG
TCCACAAAGG
AGGTGCTTGA
TGCGACCCTG
ATTCATCAAT
CCATCACTGG
GCTCTATGAA
ACCCGTATCG
ACCTTAGTCA
ACTGGGGGGC
GACCCTCCCA
AGAAGAAGCG
CAAGGTCTGA
GCGGACATTC
GATTTATGCC
GTTATGACTT
CCTTAAAAAA
GCCTTTACGA
ATGAAAGAAA
TGGAATTAGA
CTTGTTATGT
AGTTGATTCT
ACAATGGATT
ATGATTCCTG
AACTTCAAAT
CCGCTGTTCA
TTATTAATCT
CAGCTCTTCC
CGTAAAGCCA
ATGTTGAAAC
TATTCGTAAA
TGTACCTCGT
TTTGCGTGTA
CCTTGCTTAT
CACGTGATAT
TACATGACCT
GGACAGAGTT
CTGCGCGAAA
GTCATAACGT
AAATCCCGGG
CGGTAGGTGC
GTCCCGGGCG
GAAGGTAGTT
TTCTCGTCCA
CCCCAACGCG
TTTATCAACC
TCAACTTTCA
ACAACCATCA
TGCCACCAAA
AGCGCGTAAA
ACAAAGCGAG
ATTTGATTGA
GCAAGAGGGC
AGGATCCAAT
GCGCGATTCA
AGACATTAAA
AATGGAAAAT
TTCAAAAAAT
TGCGCCCGCA
GCGCGTGCAT
ACAAAATTCA
TCCCAATACA
AGTGTAATTC
CTCGTGTACT
GTGTAAGCGC
CCACTAGGTA
ATATGACATG
ATTACGAATT
CGAGCTCGGT
ACCCGGCCGG
GGATCCTCTA
GACGTGGAGT
TACCAGTGAT
TGACCAATGT
TTTATCTTCT
ACAGTTCTGC
CTGTCTACCC
CATTCTAGCT
GTACCTGACT
ACAGAGTAGT
TTAATTGTGG
TTGACCCCAC
AGTCGGAGGC
GGAGGAATAC
AGCACCGATG
TGGCCTGTCT
CCATCCAGAT
TGGCACGCAA
TTTTTACACG
CGGAAAAGAT
CGAGATAGAG
TACGACTTTA
AATTTAGTCC
CCGGCGGCTT
CTATTTTAGA
ATATTTGAGA
TTTGATTCTC
AAGCAATTGA
TTTGGTTGGG
TCACCCTCAA
TTGGATAATA
TACCTCATTG
CTCGGCTACT
TCAACTCATC
AATCACCGTC
ATACCCCGCA
TATAACCCTC
CATTCCCACG
ATGTCGTCCA
AGTCGCAATT
GACTTACGGT
GCTCGAGCCA
GCAAGCACCC
CAATCCTCTG
GCAAAGAGAC
TTTTTGAGAT
TGCCGAAGCA
AAGAAGACAA
ACGTTACCGT
CTCTGCTGAT
GTGACGACAA
CCCGAGAACT
CCTGGACCTC
GCTGACCGTA
CGGAAGCTGT
TGGATCCAAT
ACATATGCCG
TCTAGCAATG
GACTAATCAA
CTTTTGATGA
TACAGGTCTC
GGTCCCTACA
TCGCCGTCAT
CAAGACACAC
ATCGACATCC
TCACCGATTT
CAGCGTCGAC
ACTATCAATG
GCCTGAATGT
GCTGGCTCAA
AAGTACAACT
TTTTGATCTT
CGAGGACCGC
AAATTCATCG
ACATCGGCAA
TACCGTCCAG
AAGCAATACC
ACGGCGGTGC
TCTGAGGATC
TCCGAATGGG
CCCACATTAT
CAACTGCAGC
GTTCTCCCTG
GCGAGGGCAT
CGTCGAGGCT
CTGGCCCAGA
CCGCATCTGC
GCAAGACTTC
CCCTATGGTC
CTGAGAGAGG
ACTGTTGGTC
CTGGCAGAGA
TGACCCCCAA
AGGATCGCTG
GCTACGGGCG
AGTATACCAA
GGCATCGGTT
GACTACGCTC
GCAAATACAA
GAACTTCGTT
ATGGGTTTCG
TGTCGACGCG
GGCCCTGACG
GAAGTGCAGT
CGGATGTGTC
TTCAGCCTCG
GAGGATGAAG
ATTTCGTGGT
CTTCACGACG
GGTGTGAACC
TCTCTTCCAA
AGGAGATAAG
CTTGGACAGC
AATACCAGAC
TCCTGCATCG
GCTATTGGAC
GCGGTGCCGA
CTTTATCATC
GCCGGTCGAG
GCATCTACGC
TGCTCCCGAC
CCGGTTGAAG
CTGCACAGCG
GTACCAGAAA
GAAGGCTGGG
AAGCTTATAT
GGCCAGAGTA
TGCGGCAAGT
CATGATTTCC
TCTTGGAGCA
AAAGTGTAGT
GCCAGTACGA
GTGTTGTGGA
GGAAGGCTGC
ATACATTGTG
CCTGTCATTA
AACGATGAGC
TCGTCCGTAT
TGGCCCCTGT
AATGCCATGT
TTTCCGCCCC
CAATCGTCAA
GGTTTTCCCT
TTGTTAGATT
CCTACCAGTC
ATCTAGCAAG
GCGGCCGCAG
CTAGCACAAT
TGAGGCATCC
CCACTACCGC
ATTAAGACCT
CAGCGCGGCC
GCAAATTTAA
ATAAAATGAA
GTGAAGTTCC
TATACTTTCT
AGAGAATAGG
AACTTCTATA
GTGAGTCGAA
TAAGGGCGAC
ACAAAATTTA
TTCTAAATGC
ATAATAAATA
CTGATAACAT
CTTATAGTTT
GTATTATATT
TTGTATTATC
GTTGACATGT
ATAATTTTGA
TATCAAAAAC
TGATTTTCCC
TTTATTATTT
TCGAGATTTA
TTTTCTTAAT
TCTCTTTAAC
AAACTAGAAA
TATTGTATAT
ACAAAAAATC
ATAAATAATA
GATGAATAGT
TTAATTATAG
GTGTTCATCA
ATCGAAAAAG
CAACGTATCT
TATTTAAAGT
GCGTTGCTTT
TTTCTCATTT
ATAAGGTTAA
ATAATTCTCA
TATATCAAGC
AAAGTGACAG
GCGCCCTTAA
ATATTCTGAC
AAATGCTCTT
TCCCTAAACT
CCCCCCATAA
AAAAACCCGC
CGAAGCGGGT
TTTTACGTTA
TTTGCGGATT
AACGATTACT
CGTTATCAGA
ACCGCCCAGG
GGGCCCGAGC
TTAAGACTGG
CCGTCGTTTT
ACAACACAGA
AAGAGTTTGT
AGAAACGCAA
AAAGGCCATC
CGTCAGGGGC
CTTCTGCTTA
GTTTGATGCC
TGGCAGTTCC
CTACTCTCGC
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGG
CTAACTACGG
CTACACTAGA
AGAACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGACGC
GCGCGTAACT
CACGTTAAGG
GATTTTGGTC
ATGAGCTTGC
GCCGTCCCGT
CAAGTCAGCG
TAATGCTCTG
CTT