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pBE2R-mRFP1

产品货号:P56721

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pBE2R-mRFP1
别称:
基因长:
质粒宿主: 枯草杆菌
质粒用途: 蛋白表达,分泌表达
片段类型: ORF
片段物种:
原核抗性: Amp
筛选标记:
荧光标记: 红色
启动子: p43
复制子: repB
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

GATCTCAACA

GCGGTAAGAT

CCTTGAGAGT

TTTCGCCCCG

AAGAACGTTT

TCCAATGATG

61

AGCACTTTTA

AAGTTCTGCT

ATGTGGCGCG

GTATTATCCC

GTATTGACGC

CGGGCAAGAG

121

CAACTCGGTC

GCCGCATACA

CTATTCTCAG

AATGACTTGG

TTGAGTACTC

ACCAGTCACA

181

GAAAAGCATC

TTACGGATGG

CATGACAGTA

AGAGAATTAT

GCAGTGCTGC

CATAACCATG

241

AGTGATAACA

CTGCGGCCAA

CTTACTTCTG

ACAACGATCG

GAGGACCGAA

GGAGCTAACC

301

GCTTTTTTGC

ACAACATGGG

GGATCATGTA

ACTCGCCTTG

ATCGTTGGGA

ACCGGAGCTG

361

AATGAAGCCA

TACCAAACGA

CGAGCGTGAC

ACCACGATGC

CTGTAGCAAT

GGCAACAACG

421

TTGCGCAAAC

TATTAACTGG

CGAACTACTT

ACTCTAGCTT

CCCGGCAACA

ATTAATAGAC

481

TGGATGGAGG

CGGATAAAGT

TGCAGGACCA

CTTCTGCGCT

CGGCCCTTCC

GGCTGGCTGG

541

TTTATTGCTG

ATAAATCTGG

AGCCGGTGAG

CGTGGGTCTC

GCGGTATCAT

TGCAGCACTG

601

GGGCCAGATG

GTAAGCCCTC

CCGTATCGTA

GTTATCTACA

CGACGGGGAG

TCAGGCAACT

661

ATGGATGAAC

GAAATAGACA

GATCGCTGAG

ATAGGTGCCT

CACTGATTAA

GCATTGGTAA

721

CTGTCAGACC

AAGTTTACTC

ATATATACTT

TAGATTGATT

TAAAACTTCA

TTTTTAATTT

781

AAAAGGATCT

AGGTGAAGAT

CCTTTTTGAT

AATCTCATGA

CCAAAATCCC

TTAACGTGAG

841

TTTTCGTTCC

ACTGAGCGTC

AGACCCCGTA

GAAAAGATCA

AAGGATCTTC

TTGAGATCCT

901

TTTTTTCTGC

GCGTAATCTG

CTGCTTGCAA

ACAAAAAAAC

CACCGCTACC

AGCGGTGGTT

961

TGTTTGCCGG

ATCAAGAGCT

ACCAACTCTT

TTTCCGAAGG

TAACTGGCTT

CAGCAGAGCG

1021

CAGATACCAA

ATACTGTCCT

TCTAGTGTAG

CCGTAGTTAG

GCCACCACTT

CAAGAACTCT

1081

GTAGCACCGC

CTACATACCT

CGCTCTGCTA

ATCCTGTTAC

CAGTGGCTGC

TGCCAGTGGC

1141

GATAAGTCGT

GTCTTACCGG

GTTGGACTCA

AGACGATAGT

TACCGGATAA

GGCGCAGCGG

1201

TCGGGCTGAA

CGGGGGGTTC

GTGCACACAG

CCCAGCTTGG

AGCGAACGAC

CTACACCGAA

1261

CTGAGATACC

TACAGCGTGA

GCTATGAGAA

AGCGCCACGC

TTCCCGAAGG

GAGAAAGGCG

1321

GACAGGTATC

CGGTAAGCGG

CAGGGTCGGA

ACAGGAGAGC

GCACGAGGGA

GCTTCCAGGG

1381

GGAAACGCCT

GGTATCTTTA

TAGTCCTGTC

GGGTTTCGCC

ACCTCTGACT

TGAGCGTCGA

1441

TTTTTGTGAT

GCTCGTCAGG

GGGGCGGAGC

CTATGGAAAA

ACGCCAGCAA

CGCGGCCTTT

1501

TTACGGTTCC

TGGCCTTTTG

CTGGCCTTTT

GCTCACATGT

TCTTTCCTGC

GTTATCCCCT

1561

GATTCTGTGG

ATAACCGTAT

TACCGCCTTT

GAGTGAGCTG

ATACCGCTCG

CCGCAGCCGA

1621

ACGACCGAGC

GCAGCGAGTC

AGTGAGCGAG

GAAGCGGAAG

AGCGCCCAAT

ACGCAAACCG

1681

CCTCTCCCCG

CGCGTTGGCC

GATTCATTAA

TGCAGCTGAT

TTCACTTTTT

GCATTCTACA

1741

AACTGCATAA

CTCATATGTA

AATCGCTCCT

TTTTAGGTGG

CACAAATGTG

AGGCATTTTC

1801

GCTCTTTCCG

GCAACCACTT

CCAAGTAAAG

TATAACACAC

TATACTTTAT

ATTCATAAAG

1861

TGTGTGCTCT

GCGAGGCTGT

CGGCAGTGCC

GACCAAAACC

ATAAAACCTT

TAAGACCTTT

1921

CTTTTTTTTA

CGAGAAAAAA

GAAACAAAAA

AACCTGCCCT

CTGCCACCTC

AGCAAAGGGG

1981

GGTTTTGCTC

TCGTGCTCGT

TTAAAAATCA

GCAAGGGACA

GGTAGTATTT

TTTGAGAAGA

2041

TCACTCAAAA

AATCTCCACC

TTTAAACCCT

TGCCAATTTT

TATTTTGTCC

GTTTTGTCTA

2101

GCTTACCGAA

AGCCAGACTC

AGCAAGAATA

AAATTTTTAT

TGTCTTTCGG

TTTTCTAGTG

2161

TAACGGACAA

AACCACTCAA

AATAAAAAAG

ATACAAGAGA

GGTCTCTCGT

ATCTTTTATT

2221

CAGCAATCGC

GCCCGATTGC

TGAACAGATT

AATAATAGAT

TTTAGCTTTT

TATTTGTTGA

2281

AAAAAGCTAA

TCAAATTGTT

GTCGGGATCA

ATTACTGCAA

AGTCTCGTTC

ATCCCACCAC

2341

TGATCTTTTA

ATGATGTATT

GGGGTGCAAA

ATGCCCAAAG

GCTTAATATG

TTGATATAAT

2401

TCATCAATTC

CCTCTACTTC

AATGCGGCAA

CTAGCAGTAC

CAGCAATAAA

CGACTCCGCA

2461

CCTGTACAAA

CCGGTGAATC

ATTACTACGA

GAGCGCCAGC

TTCATCACTT

GCCTCCCATA

2521

GATGAATCCG

AACCTCATTA

CACATTAGAA

CTGCGAATCC

ATCTTCATGG

TGAACCAAAG

2581

TGAAACCTAG

TTTATCGCAA

TAAAAACCTA

TACTCTTTTT

AATATCCCCG

ACTGGCAATG

2641

CCGGGATAGA

CTGTAACATT

CTCACGCATA

AAATCCCCTT

TCATTTTCTA

ATGTAAATCT

2701

ATTACCTTAT

TATTAATTCA

ATTCGCTCAT

AATTAATCCT

TTTTCTTATT

ACGCAAAATG

2761

GCCCGATTTA

AGCACACCCT

TTATTCCGTT

AATGCGCCAT

GACAGCCATG

ATAATTACTA

2821

ATACTAGGAG

AAGTTAATAA

ATACGTAACC

AACATGATTA

ACAATTATTA

GAGGTCATCG

2881

TTCAAAATGG

TATGCGTTTT

GACACATCCA

CTATATATCC

GTGTCGTTCT

GTCCACTCCT

2941

GAATCCCATT

CCAGAAATTC

TCTAGCGATT

CCAGAAGTTT

CTCAGAGTCG

GAAAGTTGAC

3001

CAGACATTAC

GAACTGGCAC

AGATGGTCAT

AACCTGAAGG

AAGATCTGAT

TGCTTAACTG

3061

CTTCAGTTAA

GACCGAAGCG

CTCGTCGTAT

AACAGATGCG

ATGATGCAGA

CCAATCAACA

3121

TGGCACCTGC

CATTGCTACC

TGTACAGTCA

AGGATGGTAG

AAATGTTGTC

GGTCCTTGCA

3181

CACGAATATT

ACGCCATTTG

CCTGCATATT

CAAACAGCTC

TTCTACGATA

AGGGCACAAA

3241

TCGCATCGTG

GAACGTTTGG

GCTTCTACCG

ATTTAGCAGT

TTGATACACT

TTCTCTAAGT

3301

ATCCACCTGA

ATCATAAATC

GGCAAAATAG

AGAAAAATTG

ACCATGTGTA

AGCGGCCAAT

3361

CTGATTCCAC

CTGAGATGCA

TAATCTAGTA

GAATCTCTTC

GCTATCAAAA

TTCACTTCCA

3421

CCTTCCACTC

ACCGGTTGTC

CATTCATGGC

TGAACTCTGC

TTCCTCTGTT

GACATGACAC

3481

ACATCATCTC

AATATCCGAA

TAGGGCCACA

GTCGGCATTA

TCTCATATTA

TAAAAGCCAG

3541

TCATTAGGCC

TATCTGACAA

TTCCTGAATA

GAGTTCATAA

ACAATCCTGC

ATGATAACCA

3601

TCACAAACAG

AATGATGTAC

CTGTAAAGAT

AGCGGTAAAT

ATATTGAATT

ACCTTTATTA

3661

ATGAATTTTC

CTGCTGTAAT

AATGGGTAGA

AGGTAATTAC

TATTATTATT

GATATTTAAG

3721

TTAAACCCAG

TAAATGAAGT

CCATGGAATA

ATAGAAAGAG

AAAAAGCATT

TTCAGGTATA

3781

GGTGTTTTGG

GAAACAATTT

CCCCGAACCA

TTATATTTCT

CTACATCAGA

AAGGTATAAA

3841

TCATAAAACT

CTTTGAAGTC

ATTCTTTACA

GGAGTCCAAA

TACCAGAGAA

TGTTTTAGAT

3901

ACACCATCAA

AAATTGTATA

AAGTGGCTCT

AACTTATCCC

AATAACCTAA

CTCTCCGTCG

3961

CTATTGTAAC

CAGTTCTAAA

AGCTGTATTT

GAGTTTATCA

CCCTTGTCAC

TAAGAAAATA

4021

AATGCAGGGT

AAAATTTATA

TCCTTCTTGT

TTTATGTTTC

GGTATAAAAC

ACTAATATCA

4081

ATTTCTGTGG

TTATACTAAA

AGTCGTTTGT

TGGTTCAAAT

AATGATTAAA

TATCTCTTTT

4141

CTCTTCCAAT

TGTCTAAATC

AATTTTATTA

AAGTTCATTT

GATATGCCTC

CTGGCCCATC

4201

AGTCTGACGA

CCAAGAGAGC

CATAAACACC

AATAGCCTTA

ACATCATCCC

CATATTTATC

4261

CAATATTCGT

TCCTTAATTT

CATGAACAAT

CTTCATTCTT

TCTTCTCTAG

TCATTATTAT

4321

TGGTCCATTC

ACTATTCTCA

TTCCCTTTTC

AGATAATTTT

AGATTTGCTT

TTCTAAATAA

4381

GAATATTTGG

AGAGCACCGT

TCTTATTCAG

CTATTAATAA

CTCGTCTTCC

TAAGCATCCT

4441

TCAATCCTTT

TAATAACAAT

TATAGCATCT

AATCTTCAAC

AAACTGGCCC

GTTTGTTGAA

4501

CTACTCTTTA

ATAAAATAAT

TTTTCCGTTC

CCAATTCCAC

ATTGCAATAA

TAGAAAATCC

4561

ATCTTCATCG

GCTTTTTCGT

CATCATCTGT

ATGAATCAAA

TCGCCTTCTT

CTGTGTCATC

4621

AAGGTTTAAT

TTTTTATGTA

TTTCTTTTAA

CAAACCACCA

TAGGAGATTA

ACCTTTTACG

4681

GTGTAAACCT

TCCTCCAAAT

CAGACAAACG

TTTCAAATTC

TTTTCTTCAT

CATCGGTCAT

4741

AAAATCCGTA

TCCTTTACAG

GATATTTTGC

AGTTTCGTCA

ATTGCCGATT

GTATATCCGA

4801

TTTATATTTA

TTTTTCGGTC

GAATCATTTG

AACTTTTACA

TTTGGATCAT

AGTCTAATTT

4861

CATTGCCTTT

TTCCAAAATT

GAATCCATTG

TTTTTGATTC

ACGTAGTTTT

CTGTATTCTT

4921

AAAATAAGTT

GGTTCCACAC

ATACCAATAC

ATGCATGTGC

TGATTATAAG

AATTATCTTT

4981

ATTATTTATT

GTCACTTCCG

TTGCACGCAT

AAAACCAACA

AGATTTTTAT

TAATTTTTTT

5041

ATATTGCATC

ATTCGGCGAA

ATCCTTGAGC

CATATCTGAC

AAACTCTTAT

TTAATTCTTC

5101

GCCATCATAA

ACATTTTTAA

CTGTTAATGT

GAGAAACAAC

CAACGAACTG

TTGGCTTTTG

5161

TTTAATAACT

TCAGCAACAA

CCTTTTGTGA

CTGAATGCCA

TGTTTCATTG

CTCTCCTCCA

5221

GTTGCACATT

GGACAAAGCC

TGGATTTACA

AAACCACACT

CGATACAACT

TTCTTTCGCC

5281

TGTTTCACGA

TTTTGTTTAT

ACTCTAATAT

TTCAGCACAA

TCTTTTACTC

TTTCAGCCTT

5341

TTTAAATTCA

AGAATATGCA

GAAGTTCAAA

GTAATCAACA

TTAGCGATTT

TCTTTTCTCT

5401

CCATGGTCTC

ACTTTTCCAC

TTTTTGTCTT

GTCCACTAAA

ACCCTTGATT

TTTCATCTGA

5461

ATAAATGCTA

CTATTAGGAC

ACATAATATT

AAAAGAAACC

CCCATCTATT

TAGTTATTTG

5521

TTTAGTCACT

TATAACTTTA

ACAGATGGGG

TTTTTCTGTG

CAACCAATTT

TAAGGGTTTT

5581

CAATACTTTA

AAACACATAC

ATACCAACAC

TTCAACGCAC

CTTTCAGCAA

CTAAAATAAA

5641

AATGACGTTA

TTTCTATATG

TATCAAGATA

AGAAAGAACA

AGTTCAAAAC

CATCAAAAAA

5701

AGACACCTTT

TCAGGTGCTT

TTTTTATTTT

ATAAACTCAT

TCCCTGATCT

CGACTTCGTT

5761

CTTTTTTTAC

CTCTCGGTTA

TGAGTTAGTT

CAAATTCGTT

CTTTTTAGGT

TCTAAATCGT

5821

GTTTTTCTTG

GAATTGTGCT

GTTTTATCCT

TTACCTTGTC

TACAAACCCC

TTAAAAACGT

5881

TTTTAAAGGC

TTTTAAGCCG

TCTGTACGTT

CCTTAAGGAA

TTCGAGCTCA

GCATTATTGA

5941

GTGGATGATT

ATATTCCTTT

TGATAGGTGG

TATGTTTTCG

CTTGAACTTT

TAAATACAGC

6001

CATTGAACAT

ACGGTTGATT

TAATAACTGA

CAAACATCAC

CCTCTTGCTA

AAGCGGCCAA

6061

GGACGCTGCC

GCCGGGGCTG

TTTGCGTTTT

TGCCGTGATT

TCGTGTATTA

TTGGTTTACT

6121

TATTTTTTTG

CCAAAGCTGT

AATGGCTGAA

AATTCTTACA

TTTATTTTAC

ATTTTTAGAA

6181

ATGGGCGTGA

AAAAAAGCGC

GCGATTATGT

AAAATATAAA

GTGATAGCGG

TACCATTATA

6241

CACACATACT

AGAGAAAGAG

GAGAAATACT

AGATGGCTTC

CTCCGAAGAC

GTTATCAAAG

6301

AGTTCATGCG

TTTCAAAGTT

CGTATGGAAG

GTTCCGTTAA

CGGTCACGAG

TTCGAAATCG

6361

AAGGTGAAGG

TGAAGGTCGT

CCGTACGAAG

GTACCCAGAC

CGCTAAACTG

AAAGTTACCA

6421

AAGGTGGTCC

GCTGCCGTTC

GCTTGGGACA

TCCTGTCCCC

GCAGTTCCAG

TACGGTTCCA

6481

AAGCTTACGT

TAAACACCCG

GCTGACATCC

CGGACTACCT

GAAACTGTCC

TTCCCGGAAG

6541

GTTTCAAATG

GGAACGTGTT

ATGAACTTCG

AAGACGGTGG

TGTTGTTACC

GTTACCCAGG

6601

ACTCCTCCCT

GCAAGACGGT

GAGTTCATCT

ACAAAGTTAA

ACTGCGTGGT

ACCAACTTCC

6661

CGTCCGACGG

TCCGGTTATG

CAGAAAAAAA

CCATGGGTTG

GGAAGCTTCC

ACCGAACGTA

6721

TGTACCCGGA

AGACGGTGCT

CTGAAAGGTG

AAATCAAAAT

GCGTCTGAAA

CTGAAAGACG

6781

GTGGTCACTA

CGACGCTGAA

GTTAAAACCA

CCTACATGGC

TAAAAAACCG

GTTCAGCTGC

6841

CGGGTGCTTA

CAAAACCGAC

ATCAAACTGG

ACATCACCTC

CCACAACGAA

GACTACACCA

6901

TCGTTGAACA

GTACGAACGT

GCTGAAGGTC

GTCACTCCAC

CGGTGCTTAA

TAACGCTGAT

6961

AGTGCTAGTG

TAGATCGCTA

CTAGAGCCAG

GCATCAAATA

AAACGAAAGG

CTCAGTCGAA

7021

AGACTGGGCC

TTTCGTTTTA

TCTGTTGTTT

GTCGGTGAAC

GCTCTCTACT

AGAGTCACAC

7081

TGGCTCACCT

TCGGGTGGGC

CTTTCTGCGT

TTATATAGGC

CATCTGGGCC

GGGGGCGTCT

7141

CTGAGCTAGA

AATAGCAAGC

TTGTATTCTA

TAGTGTCACC

TAAATCGTAT

GTGTATGATA

7201

CATAAGGTTA

TGTATTAATT

GTAGCCGCGT

TCTAACGACA

ATATGTACAA

GCCTAATTGT

7261

GTAGCATCTG

GCTTACTGAA

GCAGACCCTA

TCATCTCTCT

CGTAAACTGC

CGTCAGAGTC

7321

GGTTTGGTTG

GACGAACCTT

CTGAGTTTCT

GGTAACGCCG

TCCCGCACCC

GGAAATGGTC

7381

AGCGAACCAA

TCAGCAGGGT

CATCGCTAGC

CAGATCCTCT

ACGCCGGACG

CATCGTGGCC

7441

GGCATCACCG

GCGCCACAGG

TGCGGTTGCT

GGCGCCTATA

TCGCCGACAT

CACCGATGGG

7501

GAAGATCGGG

CTCGCCACTT

CGGGCTCATG

AGCGCTTGTT

TCGGCGTGGG

TATGGTGGCA

7561

GGCCCCGTGG

CCGGGGGACT

GTTGGGCGCC

ATCTCCTTGC

ACCATTCCTT

GCGGCGGCGG

7621

TGCTCAACGG

CCTCAACCTA

CTACTGGGCT

GCTTCCTAAT

GCAGGAGTCG

CATAAGGGAG

7681

AGCGTCGATA

TGGTGCACTC

TCAGTACAAT

CTGCTCTGAT

GCCGCATAGT

TAAGCCAGCC

7741

CCGACACCCG

CCAACACCCG

CTGACGCGCC

CTGACGGGCT

TGTCTGCTCC

CGGCATCCGC

7801

TTACAGACAA

GCTGTGACCG

TCTCCGGGAG

CTGCATGTGT

CAGAGGTTTT

CACCGTCATC

7861

ACCGAAACGC

GCGAGACGAA

AGGGCCTCGT

GATACGCCTA

TTTTTATAGG

TTAATGTCAT

7921

GATAATAATG

GTTTCTTAGA

CGTCAGGTGG

CACTTTTCGG

GGAAATGTGC

GCGGAACCCC

7981

TATTTGTTTA

TTTTTCTAAA

TACATTCAAA

TATGTATCCG

CTCATGAGAC

AATAACCCTG

8041

ATAAATGCTT

CAATAATATT

GAAAAAGGAA

GAGTATGAGT

ATTCAACATT

TCCGTGTCGC

8101

CCTTATTCCC

TTTTTTGCGG

CATTTTGCCT

TCCTGTTTTT

GCTCACCCAG

AAACGCTGGT

8161

GAAAGTAAAA

GATGCTGAAG

ATCAGTTGGG

TGCACGAGTG

GGTTACATCG

AACTG

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