名称: | pHSE401-AtU6-26-FPA-sgRNA1-AtU6-1-BRI1-sgRNA1-35S-Cas9-35S-DsRed2-NOS-Bar |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 拟南芥 |
原核抗性: | Kan |
筛选标记: | Hyg |
荧光标记: | |
启动子: | AtU6,35S |
复制子: | pVS1 |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pHSE401-AtU6-26-FPA-sgRNA1-AtU6-1-BRI1-sgRNA1-35S-Cas9-35S-DsRed2-NOS-Bar |
正向引物: | G51779-F1 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TAAACGCTCT
TTTCTCTTAG
GTTTACCCGC
CAATATATCC
TGTCAAACAC
TGATAGTTTA
AACTGAAGGC
GGGAAACGAC
AATCTGATCC
AAGCTCAAGC
TGCTCTAGCA
TTCGCCATTC
AGGCTGCGCA
ACTGTTGGGA
AGGGCGATCG
GTGCGGGCCT
CTTCGCTATT
ACGCCAGCTG
GCGAAAGGGG
GATGTGCTGC
AAGGCGATTA
AGTTGGGTAA
CGCCAGGGTT
TTCCCAGTCA
CGACGTTGTA
AAACGACGGC
CAGTGCCGGT
ACCTCGACTT
GCCTTCCGCA
CAATACATCA
TTTCTTCTTA
GCTTTTTTTC
TTCTTCTTCG
TTCATACAGT
TTTTTTTTGT
TTATCAGCTT
ACATTTTCTT
GAACCGTAGC
TTTCGTTTTC
TTCTTTTTAA
CTTTCCATTC
GGAGTTTTTG
TATCTTGTTT
CATAGTTTGT
CCCAGGATTA
GAATGATTAG
GCATCGAACC
TTCAAGAATT
TGATTGAATA
AAACATCTTC
ATTCTTAAGA
TATGAAGATA
ATCTTCAAAA
GGCCCCTGGG
AATCTGAAAG
AAGAGAAGCA
GGCCCATTTA
TATGGGAAAG
AACAATAGTA
TTTCTTATAT
AGGCCCATTT
AAGTTGAAAA
CAATCTTCAA
AAGTCCCACA
TCGCTTAGAT
AAGAAAACGA
AGCTGAGTTT
ATATACAGCT
AGAGTCGAAG
TAGTGATTGC
TCTTCCTCCA
GGTCATCCTG
TTTTAGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTATCAACT
TGAAAAAGTG
GCACCGAGTC
GGTGCTTTTT
TTTGCAAAAT
TTTCCAGATC
GATTTCTTCT
TCCTCTGTTC
TTCGGCCATC
GCTACCTTAG
GACCGTTATA
GTTAATTACC
CTGTTATCCC
TATTAATTAA
GAGCTCAGAA
ATCTCAAAAT
TCCGGCAGAA
CAATTTTGAA
TCTCGATCCG
TAGAAACGAG
ACGGTCATTG
TTTTAGTTCC
ACCACGATTA
TATTTGAAAT
TTACGTGAGT
GTGAGTGAGA
CTTGCATAAG
AAAATAAAAT
CTTTAGTTGG
GAAAAAATTC
AATAATATAA
ATGGGCTTGA
GAAGGAAGCG
AGGGATAGGC
CTTTTTCTAA
AATAGGCCCA
TTTAAGCTAT
TAACAATCTT
CAAAAGTACC
ACAGCGCTTA
GGTAAAGAAA
GCAGCTGAGT
TTATATATGG
TTAGAGACGA
AGTAGTGATT
GTTGGGTCAT
AACGATATCT
CGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTATCAA
CTTGAAAAAG
TGGCACCGAG
TCGGTGCTTT
TTTTCTAGAC
CCAGCTTTCT
TGTACAAGCT
TGCATGCCTG
CAGGTCAACA
TGGTGGAGCA
CGACACACTT
GTCTACTCCA
AAAATATCAA
AGATACAGTC
TCAGAAGACC
AAAGGGCAAT
TGAGACTTTT
CAACAAAGGG
TAATATCCGG
AAACCTCCTC
GGATTCCATT
GCCCAGCTAT
CTGTCACTTT
ATTGTGAAGA
TAGTGGAAAA
GGAAGGTGGC
TCCTACAAAT
GCCATCATTG
CGATAAAGGA
AAGGCCATCG
TTGAAGATGC
CTCTGCCGAC
AGTGGTCCCA
AAGATGGACC
CCCACCCACG
AGGAGCATCG
TGGAAAAAGA
AGACGTTCCA
ACCACGTCTT
CAAAGCAAGT
GGATTGATGT
GATAACATGG
TGGAGCACGA
CACACTTGTC
TACTCCAAAA
ATATCAAAGA
TACAGTCTCA
GAAGACCAAA
GGGCAATTGA
GACTTTTCAA
CAAAGGGTAA
TATCCGGAAA
CCTCCTCGGA
TTCCATTGCC
CAGCTATCTG
TCACTTTATT
GTGAAGATAG
TGGAAAAGGA
AGGTGGCTCC
TACAAATGCC
ATCATTGCGA
TAAAGGAAAG
GCCATCGTTG
AAGATGCCTC
TGCCGACAGT
GGTCCCAAAG
ATGGACCCCC
ACCCACGAGG
AGCATCGTGG
AAAAAGAAGA
CGTTCCAACC
ACGTCTTCAA
AGCAAGTGGA
TTGATGTGAT
ATCTCCACTG
ACGTAAGGGA
TGACGCACAA
TCCCACTATC
CTTCGCAAGA
CCCTTCCTCT
ATATAAGGAA
GTTCATTTCA
TTTGGAGAGG
ACCTCGACCT
CAACACAACA
TATACAAAAC
AAACGAATCT
CAAGCAATCA
AGCATTCTAC
TTCTATTGCA
GCAATTTAAA
TCATTTCTTT
TAAAGCAAAA
GCAATTTTCT
GAAAATTTTC
ACCATTTACG
AACGATACTC
GAGTAATCTA
GATGGATTAC
AAGGACCACG
ACGGGGATTA
CAAGGACCAC
GACATTGATT
ACAAGGATGA
TGATGACAAG
ATGGCTCCGA
AGAAGAAGAG
GAAGGTTGGC
ATCCACGGGG
TGCCAGCTGC
TGACAAGAAG
TACTCGATCG
GCCTCGATAT
TGGGACTAAC
TCTGTTGGCT
GGGCCGTGAT
CACCGACGAG
TACAAGGTGC
CCTCAAAGAA
GTTCAAGGTC
CTGGGCAACA
CCGATCGGCA
TTCCATCAAG
AAGAATCTCA
TTGGCGCTCT
CCTGTTCGAC
AGCGGCGAGA
CGGCTGAGGC
TACGCGGCTC
AAGCGCACCG
CCCGCAGGCG
GTACACGCGC
AGGAAGAATC
GCATCTGCTA
CCTGCAGGAG
ATTTTCTCCA
ACGAGATGGC
GAAGGTTGAC
GATTCTTTCT
TCCACAGGCT
GGAGGAGTCA
TTCCTCGTGG
AGGAGGATAA
GAAGCACGAG
CGGCATCCAA
TCTTCGGCAA
CATTGTCGAC
GAGGTTGCCT
ACCACGAGAA
GTACCCTACG
ATCTACCATC
TGCGGAAGAA
GCTCGTGGAC
TCCACAGATA
AGGCGGACCT
CCGCCTGATC
TACCTCGCTC
TGGCCCACAT
GATTAAGTTC
AGGGGCCATT
TCCTGATCGA
GGGGGATCTC
AACCCGGACA
ATAGCGATGT
TGACAAGCTG
TTCATCCAGC
TCGTGCAGAC
GTACAACCAG
CTCTTCGAGG
AGAACCCCAT
TAATGCGTCA
GGCGTCGACG
CGAAGGCTAT
CCTGTCCGCT
AGGCTCTCGA
AGTCTCGGCG
CCTCGAGAAC
CTGATCGCCC
AGCTGCCGGG
CGAGAAGAAG
AACGGCCTGT
TCGGGAATCT
CATTGCGCTC
AGCCTGGGGC
TCACGCCCAA
CTTCAAGTCG
AATTTCGATC
TCGCTGAGGA
CGCCAAGCTG
CAGCTCTCCA
AGGACACATA
CGACGATGAC
CTGGATAACC
TCCTGGCCCA
GATCGGCGAT
CAGTACGCGG
ACCTGTTCCT
CGCTGCCAAG
AATCTGTCGG
ACGCCATCCT
CCTGTCTGAT
ATTCTCAGGG
TGAACACCGA
GATTACGAAG
GCTCCGCTCT
CAGCCTCCAT
GATCAAGCGC
TACGACGAGC
ACCATCAGGA
TCTGACCCTC
CTGAAGGCGC
TGGTCAGGCA
GCAGCTCCCC
GAGAAGTACA
AGGAGATCTT
CTTCGATCAG
TCGAAGAACG
GCTACGCTGG
GTACATTGAC
GGCGGGGCCT
CTCAGGAGGA
GTTCTACAAG
TTCATCAAGC
CGATTCTGGA
GAAGATGGAC
GGCACGGAGG
AGCTGCTGGT
GAAGCTCAAT
CGCGAGGACC
TCCTGAGGAA
GCAGCGGACA
TTCGATAACG
GCAGCATCCC
ACACCAGATT
CATCTCGGGG
AGCTGCACGC
TATCCTGAGG
AGGCAGGAGG
ACTTCTACCC
TTTCCTCAAG
GATAACCGCG
AGAAGATCGA
GAAGATTCTG
ACTTTCAGGA
TCCCGTACTA
CGTCGGCCCA
CTCGCTAGGG
GCAACTCCCG
CTTCGCTTGG
ATGACCCGCA
AGTCAGAGGA
GACGATCACG
CCGTGGAACT
TCGAGGAGGT
GGTCGACAAG
GGCGCTAGCG
CTCAGTCGTT
CATCGAGAGG
ATGACGAATT
TCGACAAGAA
CCTGCCAAAT
GAGAAGGTGC
TCCCTAAGCA
CTCGCTCCTG
TACGAGTACT
TCACAGTCTA
CAACGAGCTG
ACTAAGGTGA
AGTATGTGAC
CGAGGGCATG
AGGAAGCCGG
CTTTCCTGTC
TGGGGAGCAG
AAGAAGGCCA
TCGTGGACCT
CCTGTTCAAG
ACCAACCGGA
AGGTCACGGT
TAAGCAGCTC
AAGGAGGACT
ACTTCAAGAA
GATTGAGTGC
TTCGATTCGG
TCGAGATCTC
TGGCGTTGAG
GACCGCTTCA
ACGCCTCCCT
GGGGACCTAC
CACGATCTCC
TGAAGATCAT
TAAGGATAAG
GACTTCCTGG
ACAACGAGGA
GAATGAGGAT
ATCCTCGAGG
ACATTGTGCT
GACACTCACT
CTGTTCGAGG
ACCGGGAGAT
GATCGAGGAG
CGCCTGAAGA
CTTACGCCCA
TCTCTTCGAT
GACAAGGTCA
TGAAGCAGCT
CAAGAGGAGG
AGGTACACCG
GCTGGGGGAG
GCTGAGCAGG
AAGCTCATCA
ACGGCATTCG
GGACAAGCAG
TCCGGGAAGA
CGATCCTCGA
CTTCCTGAAG
AGCGATGGCT
TCGCGAACCG
CAATTTCATG
CAGCTGATTC
ACGATGACAG
CCTCACATTC
AAGGAGGATA
TCCAGAAGGC
TCAGGTGAGC
GGCCAGGGGG
ACTCGCTGCA
CGAGCATATC
GCGAACCTCG
CTGGCTCGCC
AGCTATCAAG
AAGGGGATTC
TGCAGACCGT
GAAGGTTGTG
GACGAGCTGG
TGAAGGTCAT
GGGCAGGCAC
AAGCCTGAGA
ACATCGTCAT
TGAGATGGCC
CGGGAGAATC
AGACCACGCA
GAAGGGCCAG
AAGAACTCAC
GCGAGAGGAT
GAAGAGGATC
GAGGAGGGCA
TTAAGGAGCT
GGGGTCCCAG
ATCCTCAAGG
AGCACCCGGT
GGAGAACACG
CAGCTGCAGA
ATGAGAAGCT
CTACCTGTAC
TACCTCCAGA
ATGGCCGCGA
TATGTATGTG
GACCAGGAGC
TGGATATTAA
CAGGCTCAGC
GATTACGACG
TCGATCATAT
CGTTCCACAG
TCATTCCTGA
AGGATGACTC
CATTGACAAC
AAGGTCCTCA
CCAGGTCGGA
CAAGAACCGG
GGCAAGTCTG
ATAATGTTCC
TTCAGAGGAG
GTCGTTAAGA
AGATGAAGAA
CTACTGGCGC
CAGCTCCTGA
ATGCCAAGCT
GATCACGCAG
CGGAAGTTCG
ATAACCTCAC
AAAGGCTGAG
AGGGGCGGGC
TCTCTGAGCT
GGACAAGGCG
GGCTTCATCA
AGAGGCAGCT
GGTCGAGACA
CGGCAGATCA
CTAAGCACGT
TGCGCAGATT
CTCGACTCAC
GGATGAACAC
TAAGTACGAT
GAGAATGACA
AGCTGATCCG
CGAGGTGAAG
GTCATCACCC
TGAAGTCAAA
GCTCGTCTCC
GACTTCAGGA
AGGATTTCCA
GTTCTACAAG
GTTCGGGAGA
TCAACAATTA
CCACCATGCC
CATGACGCGT
ACCTGAACGC
GGTGGTCGGC
ACAGCTCTGA
TCAAGAAGTA
CCCAAAGCTC
GAGAGCGAGT
TCGTGTACGG
GGACTACAAG
GTTTACGATG
TGAGGAAGAT
GATCGCCAAG
TCGGAGCAGG
AGATTGGCAA
GGCTACCGCC
AAGTACTTCT
TCTACTCTAA
CATTATGAAT
TTCTTCAAGA
CAGAGATCAC
TCTGGCCAAT
GGCGAGATCC
GGAAGCGCCC
CCTCATCGAG
ACGAACGGCG
AGACGGGGGA
GATCGTGTGG
GACAAGGGCA
GGGATTTCGC
GACCGTCAGG
AAGGTTCTCT
CCATGCCACA
AGTGAATATC
GTCAAGAAGA
CAGAGGTCCA
GACTGGCGGG
TTCTCTAAGG
AGTCAATTCT
GCCTAAGCGG
AACAGCGACA
AGCTCATCGC
CCGCAAGAAG
GACTGGGATC
CGAAGAAGTA
CGGCGGGTTC
GACAGCCCCA
CTGTGGCCTA
CTCGGTCCTG
GTTGTGGCGA
AGGTTGAGAA
GGGCAAGTCC
AAGAAGCTCA
AGAGCGTGAA
GGAGCTGCTG
GGGATCACGA
TTATGGAGCG
CTCCAGCTTC
GAGAAGAACC
CGATCGATTT
CCTGGAGGCG
AAGGGCTACA
AGGAGGTGAA
GAAGGACCTG
ATCATTAAGC
TCCCCAAGTA
CTCACTCTTC
GAGCTGGAGA
ACGGCAGGAA
GCGGATGCTG
GCTTCCGCTG
GCGAGCTGCA
GAAGGGGAAC
GAGCTGGCTC
TGCCGTCCAA
GTATGTGAAC
TTCCTCTACC
TGGCCTCCCA
CTACGAGAAG
CTCAAGGGCA
GCCCCGAGGA
CAACGAGCAG
AAGCAGCTGT
TCGTCGAGCA
GCACAAGCAT
TACCTCGACG
AGATCATTGA
GCAGATTTCC
GAGTTCTCCA
AGCGCGTGAT
CCTGGCCGAC
GCGAATCTGG
ATAAGGTCCT
CTCCGCGTAC
AACAAGCACC
GCGACAAGCC
AATCAGGGAG
CAGGCTGAGA
ATATCATTCA
TCTCTTCACC
CTGACGAACC
TCGGCGCCCC
TGCTGCTTTC
AAGTACTTCG
ACACAACTAT
CGATCGCAAG
AGGTACACAA
GCACTAAGGA
GGTCCTGGAC
GCGACCCTCA
TCCACCAGTC
GATTACCGGC
CTCTACGAGA
CGCGCATCGA
CCTGTCTCAG
CTCGGGGGCG
ACAAGCGGCC
AGCGGCGACG
AAGAAGGCGG
GGCAGGCGAA
GAAGAAGAAG
TGACTCCAAA
AATATCAAAG
ATACAGTCTC
AGAAGACCAA
AGGGCAATTG
AGACTTTTCA
ACAAAGGGTA
ATATCCGGAA
ACCTCCTCGG
ATTCCATTGC
CCAGCTATCT
GTCACTTTAT
TGTGAAGATA
GTGGAAAAGG
AAGGTGGCTC
CTACAAATGC
CATCATTGCG
ATAAAGGAAA
GGCCATCGTT
GAAGATGCCT
CTGCCGACAG
TGGTCCCAAA
GATGGACCCC
CACCCACGAG
GAGCATCGTG
GAAAAAGAAG
ACGTTCCAAC
CACGTCTTCA
AAGCAAGTGG
ATTGATGTGA
TATCTCCACT
GACGTAAGGG
ATGACGCACA
ATCCCACTAT
CCTTCGCAAG
ACCCTTCCTC
TATATAAGGA
AGTTCATTTC
ATTTGGAGAG
AACACGGGGG
ACTCTAGAGG
ATCCCCGGGA
TGGCCTCCTC
CGAGAACGTC
ATCACCGAGT
TCATGCGCTT
CAAGGTGCGC
ATGGAGGGCA
CCGTGAACGG
CCACGAGTTC
GAGATCGAGG
GCGAGGGCGA
GGGCCGCCCC
TACGAGGGCC
ACAACACCGT
GAAGCTGAAG
GTGACCAAGG
GCGGCCCCCT
GCCCTTCGCC
TGGGACATCC
TGTCCCCCCA
GTTCCAGTAC
GGCTCCAAGG
TGTACGTGAA
GCACCCCGCC
GACATCCCCG
ACTACAAGAA
GCTGTCCTTC
CCCGAGGGCT
TCAAGTGGGA
GCGCGTGATG
AACTTCGAGG
ACGGCGGCGT
GGCGACCGTG
ACCCAGGACT
CCTCCCTGCA
GGACGGCTGC
TTCATCTACA
AGGTGAAGTT
CATCGGCGTG
AACTTCCCCT
CCGACGGCCC
CGTGATGCAG
AAGAAGACCA
TGGGCTGGGA
GGCCTCCACC
GAGCGCCTGT
ACCCCCGCGA
CGGCGTGCTG
AAGGGCGAGA
CCCACAAGGC
CCTGAAGCTG
AAGGACGGCG
GCCACTACCT
GGTGGAGTTC
AAGTCCATCT
ACATGGCCAA
GAAGCCCGTG
CAGCTGCCCG
GCTACTACTA
CGTGGACGCC
AAGCTGGACA
TCACCTCCCA
CAACGAGGAC
TACACCATCG
TGGAGCAGTA
CGAGCGCACC
GAGGGCCGCC
ACCACCTGTT
CCTGTAGGCT
CAGAGCTTTC
GTTCGTATCA
TCGGTTTCGA
CAACGTTCGT
CAAGTTCAAT
GCATCAGTTT
CATTGCGCAC
ACACCAGAAT
CCTACTGAGT
TTGAGTATTA
TGGCATTGGG
AAAACTGTTT
TTCTTGTACC
ATTTGTTGTG
CTTGTAATTT
ACTGTGTTTT
TTATTCGGTT
TTCGCTATCG
AACTGTGAAA
TGGAAATGGA
TGGAGAAGAG
TTAATGAATG
ATATGGTCCT
TTTGTTCATT
CTCAAATTAA
TATTATTTGT
TTTTTCTCTT
ATTTGTTGTG
TGTTGAATTT
GAAATTATAA
GAGATATGCA
AACATTTTGT
TTTGAGTAAA
AATGTGTCAA
ATCGTGGCCT
CTAATGACCG
AAGTTAATAT
GAGGAGTAAA
ACACTTGTAG
TTGTACCATT
ATGCTTATTC
ACTAGGCAAC
AAATATATTT
TCAGACCTAG
AAAAGCTGCA
AATGTTACTG
AATACAAGTA
TGTCCTCTTG
TGTTTTAGAC
ATTTATGAAC
TTTCCTTTAT
GTAATTTTCC
AGAATCCTTG
TCAGATTCTA
ATCATTGCTT
TATAATTATA
GTTATACTCA
TGGATTTGTA
GTTGAGTATG
AAAATATTTT
TTAATGCATT
TTATGACTTG
CCAATTGATT
GACAACGAAT
TCGTAATCAT
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGCTAG
AGCAGCTTGC
CAACATGGTG
GAGCACGACA
CTCTCGTCTA
CTCCAAGAAT
ATCAAAGATA
CAGTCTCAGA
AGACCAAAGG
GCTATGAACC
GCAACGTTGA
AGGAGCCACT
GAGCCGCGGG
TTTCTGGAGT
TTAATGAGCT
AAGCACATAC
GTCAGAAACC
ATTATTGCGC
GTTCAAAAGT
CGCCTAAGGT
CACTATCAGC
TAGCAAATAT
TTCTTGTCAA
AAATGCTCCA
CTGACGTTCC
ATAAATTCCC
CTCGGTATCC
AATTAGAGTC
TCATATTCAC
TCTCAACTCG
ATCGAGGGGA
TCTACCATGA
GCCCAGAACG
ACGCCCGGCC
GACATCCGCC
GTGCCACCGA
GGCGGACATG
CCGGCGGTCT
GCACCATCGT
CAACCACTAC
ATCGAGACAA
GCACGGTCAA
CTTCCGTACC
GAGCCGCAGG
AACCGCAGGA
GTGGACGGAC
GACCTCGTCC
GTCTGCGGGA
GCGCTATCCC
TGGCTCGTCG
CCGAGGTGGA
CGGCGAGGTC
GCCGGCATCG
CCTACGCGGG
TCCCTGGAAG
GCACGCAACG
CCTACGACTG
GACGGCCGAG
TCGACCGTGT
ACGTCTCCCC
CCGCCACCAG
CGGACGGGAC
TGGGCTCCAC
GCTCTACACC
CACCTGCTGA
AGTCCCTGGA
GGCACAGGGC
TTCAAGAGCG
TGGTCGCTGT
CATCGGGCTG
CCCAACGACC
CGAGCGTGCG
CATGCACGAG
GCGCTCGGAT
ATGCCCCCCG
CGGCATGCTG
CGGGCGGCCG
GCTTCAAGCA
CGGGAACTGG
CATGACGTGG
GTTTCTGGCA
GCTGGACTTC
AGCCTGCCGG
TGCCGCCCCG
TCCGGTCCTG
CCCGTCACCG
AAATCTGATG
ACCCCTAGAG
TCAAGCAGAT
CGTTAAAACA
TTTGGCAATA
AAGTTTCTTA
AGATTGAATC
CTGTTGCCGG
TCTTGCGATG
ATTATCATAT
AATTTCTGTT
GAATTACGTT
AAGCATGTAA
TAATTAACAT
GTAATGCATG
ACGTTATTTA
TGAGATGGGT
TTTTATGATT
AGAGTCCCGC
AATTATACAT
TTAATACGCG
ATAGAAAACA
AAATATAGCG
CGCAAACTAG
GATAAATTAT
CGCGCGCGGT
GTCATCTATG
TTACTAGATC
CCGCGGTTTC
AAAATCGGCT
CCGTCGATAC
TATGTTATAC
GCCAACTTTG
AAAACAACTT
TGAAAAAGCT
GTTTTCTGGT
ATTTAAGGTT
TTAGAATGCA
AGGAACAGTG
AATTGGAGTT
CGTCTTGTTA
TAATTAGCTT
CTTGGGGTAT
CTTTAAATAC
TGTAGAAAAG
AGGAAGGAAA
TAATAAATGG
CTAAAATGAG
AATATCACCG
GAATTGAAAA
AACTGATCGA
AAAATACCGC
TGCGTAAAAG
ATACGGAAGG
AATGTCTCCT
GCTAAGGTAT
ATAAGCTGGT
GGGAGAAAAT
GAAAACCTAT
ATTTAAAAAT
GACGGACAGC
CGGTATAAAG
GGACCACCTA
TGATGTGGAA
CGGGAAAAGG
ACATGATGCT
ATGGCTGGAA
GGAAAGCTGC
CTGTTCCAAA
GGTCCTGCAC
TTTGAACGGC
ATGATGGCTG
GAGCAATCTG
CTCATGAGTG
AGGCCGATGG
CGTCCTTTGC
TCGGAAGAGT
ATGAAGATGA
ACAAAGCCCT
GAAAAGATTA
TCGAGCTGTA
TGCGGAGTGC
ATCAGGCTCT
TTCACTCCAT
CGACATATCG
GATTGTCCCT
ATACGAATAG
CTTAGACAGC
CGCTTAGCCG
AATTGGATTA
CTTACTGAAT
AACGATCTGG
CCGATGTGGA
TTGCGAAAAC
TGGGAAGAAG
ACACTCCATT
TAAAGATCCG
CGCGAGCTGT
ATGATTTTTT
AAAGACGGAA
AAGCCCGAAG
AGGAACTTGT
CTTTTCCCAC
GGCGACCTGG
GAGACAGCAA
CATCTTTGTG
AAAGATGGCA
AAGTAAGTGG
CTTTATTGAT
CTTGGGAGAA
GCGGCAGGGC
GGACAAGTGG
TATGACATTG
CCTTCTGCGT
CCGGTCGATC
AGGGAGGATA
TCGGGGAAGA
ACAGTATGTC
GAGCTATTTT
TTGACTTACT
GGGGATCAAG
CCTGATTGGG
AGAAAATAAA
ATATTATATT
TTACTGGATG
AATTGTTTTA
GTACCTAGAA
TGCATGACCA
AAATCCCTTA
ACGTGAGTTT
TCGTTCCACT
GAGCGTCAGA
CCCCGTAGAA
AAGATCAAAG
GATCTTCTTG
AGATCCTTTT
TTTCTGCGCG
TAATCTGCTG
CTTGCAAACA
AAAAAACCAC
CGCTACCAGC
GGTGGTTTGT
TTGCCGGATC
AAGAGCTACC
AACTCTTTTT
CCGAAGGTAA
CTGGCTTCAG
CAGAGCGCAG
ATACCAAATA
CTGTCCTTCT
AGTGTAGCCG
TAGTTAGGCC
ACCACTTCAA
GAACTCTGTA
GCACCGCCTA
CATACCTCGC
TCTGCTAATC
CTGTTACCAG
TGGCTGCTGC
CAGTGGCGAT
AAGTCGTGTC
TTACCGGGTT
GGACTCAAGA
CGATAGTTAC
CGGATAAGGC
GCAGCGGTCG
GGCTGAACGG
GGGGTTCGTG
CACACAGCCC
AGCTTGGAGC
GAACGACCTA
CACCGAACTG
AGATACCTAC
AGCGTGAGCT
ATGAGAAAGC
GCCACGCTTC
CCGAAGGGAG
AAAGGCGGAC
AGGTATCCGG
TAAGCGGCAG
GGTCGGAACA
GGAGAGCGCA
CGAGGGAGCT
TCCAGGGGGA
AACGCCTGGT
ATCTTTATAG
TCCTGTCGGG
TTTCGCCACC
TCTGACTTGA
GCGTCGATTT
TTGTGATGCT
CGTCAGGGGG
GCGGAGCCTA
TGGAAAAACG
CCAGCAACGC
GGCCTTTTTA
CGGTTCCTGG
CCTTTTGCTG
GCCTTTTGCT
CACATGTTCT
TTCCTGCGTT
ATCCCCTGAT
TCTGTGGATA
ACCGTATTAC
CGCCTTTGAG
TGAGCTGATA
CCGCTCGCCG
CAGCCGAACG
ACCGAGCGCA
GCGAGTCAGT
GAGCGAGGAA
GCGGAAGAGC
GCCTGATGCG
GTATTTTCTC
CTTACGCATC
TGTGCGGTAT
TTCACACCGC
ATATGGTGCA
CTCTCAGTAC
AATCTGCTCT
GATGCCGCAT
AGTTAAGCCA
GTATACACTC
CGCTATCGCT
ACGTGACTGG
GTCATGGCTG
CGCCCCGACA
CCCGCCAACA
CCCGCTGACG
CGCCCTGACG
GGCTTGTCTG
CTCCCGGCAT
CCGCTTACAG
ACAAGCTGTG
ACCGTCTCCG
GGAGCTGCAT
GTGTCAGAGG
TTTTCACCGT
CATCACCGAA
ACGCGCGAGG
CAGGGTGCCT
TGATGTGGGC
GCCGGCGGTC
GAGTGGCGAC
GGCGCGGCTT
GTCCGCGCCC
TGGTAGATTG
CCTGGCCGTA
GGCCAGCCAT
TTTTGAGCGG
CCAGCGGCCG
CGATAGGCCG
ACGCGAAGCG
GCGGGGCGTA
GGGAGCGCAG
CGACCGAAGG
GTAGGCGCTT
TTTGCAGCTC
TTCGGCTGTG
CGCTGGCCAG
ACAGTTATGC
ACAGGCCAGG
CGGGTTTTAA
GAGTTTTAAT
AAGTTTTAAA
GAGTTTTAGG
CGGAAAAATC
GCCTTTTTTC
TCTTTTATAT
CAGTCACTTA
CATGTGTGAC
CGGTTCCCAA
TGTACGGCTT
TGGGTTCCCA
ATGTACGGGT
TCCGGTTCCC
AATGTACGGC
TTTGGGTTCC
CAATGTACGT
GCTATCCACA
GGAAACAGAC
CTTTTCGACC
TTTTTCCCCT
GCTAGGGCAA
TTTGCCCTAG
CATCTGCTCC
GTACATTAGG
AACCGGCGGA
TGCTTCGCCC
TCGATCAGGT
TGCGGTAGCG
CATGACTAGG
ATCGGGCCAG
CCTGCCCCGC
CTCCTCCTTC
AAATCGTACT
CCGGCAGGTC
ATTTGACCCG
ATCAGCTTGC
GCACGGTGAA
ACAGAACTTC
TTGAACTCTC
CGGCGCTGCC
ACTGCGTTCG
TAGATCGTCT
TGAACAACCA
TCTGGCTTCT
GCCTTGCCTG
CGGCGCGGCG
TGCCAGGCGG
TAGAGAAAAC
GGCCGATGCC
GGGATCGATC
AAAAAGTAAT
CGGGGTGAAC
CGTCAGCACG
TCCGGGTTCT
TGCCTTCTGT
GATCTCGCGG
TACATCCAAT
CAGCTAGCTC
GATCTCGATG
TACTCCGGCC
GCCCGGTTTC
GCTCTTTACG
ATCTTGTAGC
GGCTAATCAA
GGCTTCACCC
TCGGATACCG
TCACCAGGCG
GCCGTTCTTG
GCCTTCTTCG
TACGCTGCAT
GGCAACGTGC
GTGGTGTTTA
ACCGAATGCA
GGTTTCTACC
AGGTCGTCTT
TCTGCTTTCC
GCCATCGGCT
CGCCGGCAGA
ACTTGAGTAC
GTCCGCAACG
TGTGGACGGA
ACACGCGGCC
GGGCTTGTCT
CCCTTCCCTT
CCCGGTATCG
GTTCATGGAT
TCGGTTAGAT
GGGAAACCGC
CATCAGTACC
AGGTCGTAAT
CCCACACACT
GGCCATGCCG
GCCGGCCCTG
CGGAAACCTC
TACGTGCCCG
TCTGGAAGCT
CGTAGCGGAT
CACCTCGCCA
GCTCGTCGGT
CACGCTTCGA
CAGACGGAAA
ACGGCCACGT
CCATGATGCT
GCGACTATCG
CGGGTGCCCA
CGTCATAGAG
CATCGGAACG
AAAAAATCTG
GTTGCTCGTC
GCCCTTGGGC
GGCTTCCTAA
TCGACGGCGC
ACCGGCTGCC
GGCGGTTGCC
GGGATTCTTT
GCGGATTCGA
TCAGCGGCCG
CTTGCCACGA
TTCACCGGGG
CGTGCTTCTG
CCTCGATGCG
TTGCCGCTGG
GCGGCCTGCG
CGGCCTTCAA
CTTCTCCACC
AGGTCATCAC
CCAGCGCCGC
GCCGATTTGT
ACCGGGCCGG
ATGGTTTGCG
ACCGCTCACG
CCGATTCCTC
GGGCTTGGGG
GTTCCAGTGC
CATTGCAGGG
CCGGCAGGCA
ACCCAGCCGC
TTACGCCTGG
CCAACCGCCC
GTTCCTCCAC
ACATGGGGCA
TTCCACGGCG
TCGGTGCCTG
GTTGTTCTTG
ATTTTCCATG
CCGCCTCCTT
TAGCCGCTAA
AATTCATCTA
CTCATTTATT
CATTTGCTCA
TTTACTCTGG
TAGCTGCGCG
ATGTATTCAG
ATAGCAGCTC
GGTAATGGTC
TTGCCTTGGC
GTACCGCGTA
CATCTTCAGC
TTGGTGTGAT
CCTCCGCCGG
CAACTGAAAG
TTGACCCGCT
TCATGGCTGG
CGTGTCTGCC
AGGCTGGCCA
ACGTTGCAGC
CTTGCTGCTG
CGTGCGCTCG
GACGGCCGGC
ACTTAGCGTG
TTTGTGCTTT
TGCTCATTTT
CTCTTTACCT
CATTAACTCA
AATGAGTTTT
GATTTAATTT
CAGCGGCCAG
CGCCTGGACC
TCGCGGGCAG
CGTCGCCCTC
GGGTTCTGAT
TCAAGAACGG
TTGTGCCGGC
GGCGGCAGTG
CCTGGGTAGC
TCACGCGCTG
CGTGATACGG
GACTCAAGAA
TGGGCAGCTC
GTACCCGGCC
AGCGCCTCGG
CAACCTCACC
GCCGATGCGC
GTGCCTTTGA
TCGCCCGCGA
CACGACAAAG
GCCGCTTGTA
GCCTTCCATC
CGTGACCTCA
ATGCGCTGCT
TAACCAGCTC
CACCAGGTCG
GCGGTGGCCC
ATATGTCGTA
AGGGCTTGGC
TGCACCGGAA
TCAGCACGAA
GTCGGCTGCC
TTGATCGCGG
ACACAGCCAA
GTCCGCCGCC
TGGGGCGCTC
CGTCGATCAC
TACGAAGTCG
CGCCGGCCGA
TGGCCTTCAC
GTCGCGGTCA
ATCGTCGGGC
GGTCGATGCC
GACAACGGTT
AGCGGTTGAT
CTTCCCGCAC
GGCCGCCCAA
TCGCGGGCAC
TGCCCTGGGG
ATCGGAATCG
ACTAACAGAA
CATCGGCCCC
GGCGAGTTGC
AGGGCGCGGG
CTAGATGGGT
TGCGATGGTC
GTCTTGCCTG
ACCCGCCTTT
CTGGTTAAGT
ACAGCGATAA
CCTTCATGCG
TTCCCCTTGC
GTATTTGTTT
ATTTACTCAT
CGCATCATAT
ACGCAGCGAC
CGCATGACGC
AAGCTGTTTT
ACTCAAATAC
ACATCACCTT
TTTAGACGGC
GGCGCTCGGT
TTCTTCAGCG
GCCAAGCTGG
CCGGCCAGGC
CGCCAGCTTG
GCATCAGACA
AACCGGCCAG
GATTTCATGC
AGCCGCACGG
TTGAGACGTG
CGCGGGCGGC
TCGAACACGT
ACCCGGCCGC
GATCATCTCC
GCCTCGATCT
CTTCGGTAAT
GAAAAACGGT
TCGTCCTGGC
CGTCCTGGTG
CGGTTTCATG
CTTGTTCCTC
TTGGCGTTCA
TTCTCGGCGG
CCGCCAGGGC
GTCGGCCTCG
GTCAATGCGT
CCTCACGGAA
GGCACCGCGC
CGCCTGGCCT
CGGTGGGCGT
CACTTCCTCG
CTGCGCTCAA
GTGCGCGGTA
CAGGGTCGAG
CGATGCACGC
CAAGCAGTGC
AGCCGCCTCT
TTCACGGTGC
GGCCTTCCTG
GTCGATCAGC
TCGCGGGCGT
GCGCGATCTG
TGCCGGGGTG
AGGGTAGGGC
GGGGGCCAAA
CTTCACGCCT
CGGGCCTTGG
CGGCCTCGCG
CCCGCTCCGG
GTGCGGTCGA
TGATTAGGGA
ACGCTCGAAC
TCGGCAATGC
CGGCGAACAC
GGTCAACACC
ATGCGGCCGG
CCGGCGTGGT
GGTGTCGGCC
CACGGCTCTG
CCAGGCTACG
CAGGCCCGCG
CCGGCCTCCT
GGATGCGCTC
GGCAATGTCC
AGTAGGTCGC
GGGTGCTGCG
GGCCAGGCGG
TCTAGCCTGG
TCACTGTCAC
AACGTCGCCA
GGGCGTAGGT
GGTCAAGCAT
CCTGGCCAGC
TCCGGGCGGT
CGCGCCTGGT
GCCGGTGATC
TTCTCGGAAA
ACAGCTTGGT
GCAGCCGGCC
GCGTGCAGTT
CGGCCCGTTG
GTTGGTCAAG
TCCTGGTCGT
CGGTGCTGAC
GCGGGCATAG
CCCAGCAGGC
CAGCGGCGGC
GCTCTTGTTC
ATGGCGTAAT
GTCTCCGGTT
CTAGTCGCAA
GTATTCTACT
TTATGCGACT
AAAACACGCG
ACAAGAAAAC
GCCAGGAAAA
GGGCAGGGCG
GCAGCCTGTC
GCGTAACTTA
GGACTTGTGC
GACATGTCGT
TTTCAGAAGA
CGGCTGCACT
GAACGTCAGA
AGCCGACTGC
ACTATAGCAG
CGGAGGGGTT
GGATCAAAGT
ACTTTGATCC
CGAGGGGAAC
CCTGTGGTTG
GCATGCACAT
ACAAATGGAC
GAACGGATAA
ACCTTTTCAC
GCCCTTTTAA
ATATCCGATT
ATTCTAA