名称: | PiggyBac-CAG-CD163(pig)-CopGFP-Puro |
---|---|
别称: | - |
基因长: | 3348bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达,转座 |
片段类型: | ORF |
片段物种: | 猪 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CAG |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | PiggyBac Dual promoter-CAG |
正向引物: | pcaggs-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
PiggyBac-CAG-CD163(pig)-CopGFP-Puro是适用于哺乳细胞过表达和转座的质粒。质粒包含PiggyBac转座系统,便于将猪源CD163基因稳定地整合到宿主细胞的基因组中。质粒带有的CAG强启动子可启动猪源CD163基因转录。质粒携带的CopGFP绿色荧光蛋由EF1a启动子启动表达,便于观察目的基因的表达和定位情况。质粒携带Puro抗性基因,用于筛选出表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
AAATTGTAAG
CGTTAATATT
TTGTTAAAAT
TCGCGTTAAA
TTTTTGTTAA
ATCAGCTCAT
TTTTTAACCA
ATAGGCCGAA
ATCGGCAAAA
TCCCTTATAA
ATCAAAAGAA
TAGACCGAGA
TAGGGTTGAG
TGTTGTTCCA
GTTTGGAACA
AGAGTCCACT
ATTAAAGAAC
GTGGACTCCA
ACGTCAAAGG
GCGAAAAACC
GTCTATCAGG
GCGATGGCCC
ACTACGTGAA
CCATCACCCT
AATCAAGTTT
TTTGGGGTCG
AGGTGCCGTA
AAGCACTAAA
TCGGAACCCT
AAAGGGAGCC
CCCGATTTAG
AGCTTGACGG
GGAAAGCCGG
CGAACGTGGC
GAGAAAGGAA
GGGAAGAAAG
CGAAAGGAGC
GGGCGCTAGG
GCGCTGGCAA
GTGTAGCGGT
CACGCTGCGC
GTAACCACCA
CACCCGCCGC
GCTTAATGCG
CCGCTACAGG
GCGCGTCCCA
TTCGCCATTC
AGGCTGCGCA
ACTGTTGGGA
AGGGCGATCG
GTGCGGGCCT
CTTCGCTATT
ACGCCAGCTG
GCGAAAGGGG
GATGTGCTGC
AAGGCGATTA
AGTTGGGTAA
CGCCAGGGTT
TTCCCAGTCA
CGACGTTGTA
AAACGACGGC
CAGTGAGCGC
GCCTCGTTCA
TTCACGTTTT
TGAACCCGTG
GAGGACGGGC
AGACTCGCGG
TGCAAATGTG
TTTTACAGCG
TGATGGAGCA
GATGAAGATG
CTCGACACGC
TGCAGAACAC
GCAGCTAGAT
TAACCCTAGA
AAGATAATCA
TATTGTGACG
TACGTTAAAG
ATAATCATGC
GTAAAATTGA
CGCATGTGTT
TTATCGGTCT
GTATATCGAG
GTTTATTTAT
TAATTTGAAT
AGATATTAAG
TTTTATTATA
TTTACACTTA
CATACTAATA
ATAAATTCAA
CAAACAATTT
ATTTATGTTT
ATTTATTTAT
TAAAAAAAAA
CAAAAACTCA
AAATTTCTTC
TATAAAGTAA
CAAAACTTTT
ATGAGGGACA
GCCCCCCCCC
AAAGCCCCCA
GGGATGTAAT
TACGTCCCTC
CCCCGCTAGG
GGGCAGCAGC
GAGCCGCCCG
GGGCTCCGCT
CCGGTCCGGC
GCTCCCCCCG
CATCCCCGAG
CCGGCAGCGT
GCGGGGACAG
CCCGGGCACG
GGGAAGGTGG
CACGGGATCG
CTTTCCTCTG
AACGCTTCTC
GCTGCTCTTT
GAGCCTGCAG
ACACCTGGGG
GGATACGGGG
AAAAGGCCTC
CAAGGCCTAC
TAGTATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGACATTGAT
TATTGACTAG
TTATTAATAG
TAATCAATTA
CGGGGTCATT
AGTTCATAGC
CCATATATGG
AGTTCCGCGT
TACATAACTT
ACGGTAAATG
GCCCGCCTGG
CTGACCGCCC
AACGACCCCC
GCCCATTGAC
GTCAATAATG
ACGTATGTTC
CCATAGTAAC
GCCAATAGGG
ACTTTCCATT
GACGTCAATG
GGTGGACTAT
TTACGGTAAA
CTGCCCACTT
GGCAGTACAT
CAAGTGTATC
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGGTCGAGGT
GAGCCCCACG
TTCTGCTTCA
CTCTCCCCAT
CTCCCCCCCC
TCCCCACCCC
CAATTTTGTA
TTTATTTATT
TTTTAATTAT
TTTGTGCAGC
GATGGGGGCG
GGGGGGGGGG
GGGCGCGCGC
CAGGCGGGGC
GGGGCGGGGC
GAGGGGCGGG
GCGGGGCGAG
GCGGAGAGGT
GCGGCGGCAG
CCAATCAGAG
CGGCGCGCTC
CGAAAGTTTC
CTTTTATGGC
GAGGCGGCGG
CGGCGGCGGC
CCTATAAAAA
GCGAAGCGCG
CGGCGGGCGG
GAGTCGCTGC
GTTGCCTTCG
CCCCGTGCCC
CGCTCCGCGC
CGCCTCGCGC
CGCCCGCCCC
GGCTCTGACT
GACCGCGTTA
CTCCCACAGG
TGAGCGGGCG
GGACGGCCCT
TCTCCTCCGG
GCTGTAATTA
GCGCTTGGTT
TAATGACGGC
TCGTTTCTTT
TCTGTGGCTG
CGTGAAAGCC
TTAAAGGGCT
CCGGGAGGGC
CCTTTGTGCG
GGGGGGAGCG
GCTCGGGGGG
TGCGTGCGTG
TGTGTGTGCG
TGGGGAGCGC
CGCGTGCGGC
CCGCGCTGCC
CGGCGGCTGT
GAGCGCTGCG
GGCGCGGCGC
GGGGCTTTGT
GCGCTCCGCG
TGTGCGCGAG
GGGAGCGCGG
CCGGGGGCGG
TGCCCCGCGG
TGCGGGGGGG
CTGCGAGGGG
AACAAAGGCT
GCGTGCGGGG
TGTGTGCGTG
GGGGGGTGAG
CAGGGGGTGT
GGGCGCGGCG
GTCGGGCTGT
AACCCCCCCC
TGCACCCCCC
TCCCCGAGTT
GCTGAGCACG
GCCCGGCTTC
GGGTGCGGGG
CTCCGTGCGG
GGCGTGGCGC
GGGGCTCGCC
GTGCCGGGCG
GGGGGTGGCG
GCAGGTGGGG
GTGCCGGGCG
GGGCGGGGCC
GCCTCGGGCC
GGGGAGGGCT
CGGGGGAGGG
GCGCGGCGGC
CCCGGAGCGC
CGGCGGCTGT
CGAGGCGCGG
CGAGCCGCAG
CCATTGCCTT
TTATGGTAAT
CGTGCGAGAG
GGCGCAGGGA
CTTCCTTTGT
CCCAAATCTG
TGCGGAGCCG
AAATCTGGGA
GGCGCCGCCG
CACCCCCTCT
AGCGGGCGCG
GGGCGAAGCG
GTGCGGCGCC
GGCAGGAAGG
AAATGGGCGG
GGAGGGCCTT
CGTGCGTCGC
CGCGCCGCCG
TCCCCTTCTC
CCTCTCCAGC
CTCGGGGCTG
TCCGCGGGGG
GACGGCTGCC
TTCGGGGGGG
ACGGGGCAGG
GCGGGGTTCG
GCTTCTGGCG
TGTGACCGGC
GGCTCTAGAG
CCTCTGCTAA
CCATGTTCAT
GCCTTCTTCT
TTTTCCTACA
GCTCCTGGGC
AACGTGCTGG
TTATTGTGCT
GTCTCATCAT
TTTGGCAAAG
AATTCTGCAG
TCGACGGTAC
CCGTTTAGTG
AACCGTCAGA
TCGCCTGGAG
ACGCCATCCA
CGCTGTTTTG
ACCTCCATAG
AAGATTCTAG
AGCTAGCCAC
CATGGACAAA
CTCAGAATGG
TGCTACATGA
AAACTCTGGA
TCTGCAGACT
TTAGAAGATG
TTCTGCCCAT
TTAAGTTCCT
TCACTTTTGC
TGTAGTCGCT
GTTCTCAGTG
CCTGCTTGGT
CACTAGTTCT
CTTGGAGGAA
AAGACAAGGA
GCTGAGGCTA
ACGGGTGGTG
AAAACAAGTG
CTCTGGAAGA
GTGGAGGTGA
AAGTGCAGGA
GGAGTGGGGA
ACTGTGTGTA
ATAATGGCTG
GGACATGGAT
GTGGTCTCTG
TTGTTTGTAG
GCAGCTGGGA
TGTCCAACTG
CTATCAAAGC
CACTGGATGG
GCTAATTTTA
GTGCAGGTTC
TGGACGCATT
TGGATGGATC
ATGTTTCTTG
TCGAGGGAAT
GAGTCAGCTC
TCTGGGACTG
CAAACATGAT
GGATGGGGAA
AGCATAACTG
TACTCACCAA
CAGGATGCTG
GAGTAACCTG
CTCAGATGGA
TCTGATTTAG
AGATGAGGCT
GGTGAATGGA
GGAAACCGGT
GCTTAGGAAG
AATAGAAGTC
AAATTTCAAG
GACGGTGGGG
AACAGTGTGT
GATGATAACT
TCAACATAAA
TCATGCTTCT
GTGGTTTGTA
AACAACTTGA
ATGTGGAAGT
GCTGTCAGTT
TCTCTGGTTC
AGCTAATTTT
GGAGAAGGTT
CTGGACCAAT
CTGGTTTGAT
GATCTTGTAT
GCAATGGAAA
TGAGTCAGCT
CTCTGGAACT
GCAAACATGA
AGGATGGGGA
AAGCACAATT
GCGATCATGC
TGAGGATGCT
GGAGTGATTT
GCTTAAATGG
AGCAGACCTG
AAACTGAGAG
TGGTAGATGG
AGTCACTGAA
TGTTCAGGAA
GATTGGAAGT
GAAATTCCAA
GGAGAATGGG
GAACAATCTG
TGATGATGGC
TGGGATAGTG
ATGATGCCGC
TGTGGCATGT
AAGCAACTGG
GATGTCCAAC
TGCTGTCACT
GCCATTGGTC
GAGTTAACGC
CAGTGAGGGA
ACTGGACACA
TTTGGCTTGA
CAGTGTTTCT
TGCCATGGAC
ACGAGTCTGC
TCTCTGGCAG
TGTAGACACC
ATGAATGGGG
AAAGCATTAT
TGCAATCATA
ATGAAGATGC
TGGTGTGACA
TGTTCTGATG
GATCAGATCT
GGAACTGAGA
CTTAAAGGTG
GAGGCAGCCA
CTGTGCTGGG
ACAGTGGAGG
TGGAAATTCA
GAAACTGGTA
GGAAAAGTGT
GTGATAGAAG
CTGGGGACTG
AAAGAAGCTG
ATGTGGTTTG
CAGGCAGCTG
GGATGTGGAT
CTGCACTCAA
AACATCATAT
CAAGTTTATT
CCAAAACCAA
GGCAACAAAC
ACATGGCTGT
TTGTAAGCAG
CTGTAATGGA
AATGAAACTT
CTCTTTGGGA
CTGCAAGAAT
TGGCAGTGGG
GTGGACTTAG
TTGTGATCAC
TATGACGAAG
CCAAAATTAC
CTGCTCAGCC
CACAGGAAAC
CCAGGCTGGT
TGGAGGGGAC
ATTCCCTGCT
CTGGTCGTGT
TGAAGTACAA
CATGGAGACA
CGTGGGGCAC
CGTCTGTGAT
TCTGACTTCT
CTCTGGAGGC
GGCCAGCGTG
CTGTGCAGGG
AACTACAGTG
CGGCACTGTG
GTTTCCCTCC
TGGGGGGAGC
TCACTTTGGA
GAAGGAAGTG
GACAGATCTG
GGCTGAAGAA
TTCCAGTGTG
AGGGGCACGA
GTCCCACCTT
TCACTCTGCC
CAGTAGCACC
CCGCCCTGAC
GGGACATGTA
GCCACAGCAG
GGACGTCGGC
GTAGTCTGCT
CAAGATACAC
ACAAATCCGC
TTGGTGAATG
GCAAGACCCC
ATGTGAAGGA
AGAGTGGAGC
TCAACATTCT
TGGGTCCTGG
GGGTCCCTCT
GCAACTCTCA
CTGGGACATG
GAAGATGCCC
ATGTTTTATG
CCAGCAGCTT
AAATGTGGAG
TTGCCCTTTC
TATCCCGGGA
GGAGCACCTT
TTGGGAAAGG
AAGTGAGCAG
GTCTGGAGGC
ACATGTTTCA
CTGCACTGGG
ACTGAGAAGC
ACATGGGAGA
TTGTTCCGTC
ACTGCTCTGG
GCGCATCACT
CTGTTCTTCA
GGGCAAGTGG
CCTCTGTAAT
CTGCTCAGGG
AACCAGAGTC
AGACACTATC
CCCGTGCAAT
TCATCATCCT
CGGACCCATC
AAGCTCTATT
ATTTCAGAAG
AAAATGGTGT
TGCCTGCATA
GGGAGTGGTC
AACTTCGCCT
GGTCGATGGA
GGTGGTCGTT
GTGCTGGGAG
AGTAGAGGTC
TATCATGAGG
GCTCCTGGGG
CACCATCTGT
GATGACAGCT
GGGACCTGAA
TGATGCCCAT
GTGGTGTGCA
AACAGCTGAG
CTGTGGATGG
GCCATTAATG
CCACTGGTTC
TGCTCATTTT
GGGGAAGGAA
CAGGGCCCAT
TTGGCTGGAT
GAGATAAACT
GTAATGGAAA
AGAATCTCAT
ATTTGGCAAT
GCCACTCACA
TGGTTGGGGG
CGGCACAATT
GCAGGCATAA
GGAGGATGCA
GGAGTCATCT
GCTCGGAGTT
CATGTCTCTC
AGACTGATCA
GTGAAAGCAG
CAGAGAGACC
TGTGCAGGGC
GCCTGGAAGT
TTTTTACAAC
GGAGCTTGGG
GCAGCGTTGG
CAAGAATAGC
ATGTCTCCAG
CCACAGTGGG
GGTGGTATGC
AGGCAGCTGG
GCTGTGCAGA
CAGAGGGGAC
ATCAGCCCTG
CATCTTCAGA
CAAGACAGTG
TCCAGGCACA
TGTGGGTGGA
CAATGTTCAG
TGTCCTAAAG
GACCTGACAC
CCTATGGCAG
TGCCCATCAT
CTCCATGGAA
GAAGAGACTG
GCCAGCCCCT
CAGAGGAGAC
ATGGATCACA
TGTGCCAACA
AAATAAGACT
TCAAGAAGGA
AACACTAATT
GTTCTGGACG
TGTGGAGATC
TGGTACGGAG
GTTCCTGGGG
CACTGTGTGT
GACGACTCCT
GGGACCTTGA
AGATGCTCAG
GTGGTGTGCC
GACAGCTGGG
CTGTGGCTCA
GCTTTGGAGG
CAGGAAAAGA
GGCCGCATTT
GGCCAGGGGA
CTGGGCCCAT
ATGGCTCAAT
GAAGTGAAGT
GCAAGGGGAA
TGAAACCTCC
TTGTGGGATT
GTCCTGCCAG
ATCCTGGGGC
CACAGTGACT
GTGGACACAA
GGAGGATGCT
GCTGTGACGT
GTTCAGAAAT
TGCAAAGAGC
CGAGAATCCC
TACATGCCAC
AGGTCGCTCA
TCTTTTGTTG
CACTTGCAAT
CTTTGGGGTC
ATTCTGTTGG
CCTGTCTCAT
CGCATTCCTC
ATTTGGACTC
AGAAGCGAAG
ACAGAGGCAG
CGGCTCTCAG
TTTTCTCAGG
AGGAGAGAAT
TCTGTCCATC
AAATTCAATA
CCGGGAGATG
AATTCTTGCC
TGAAAGCAGA
TGAAACGGAT
ATGCTAAATC
CCTCAGGAGA
CCACTCTGAA
GTACAATGAC
TCGAGCTAGC
AGATCTTAAT
TAATTAAGGA
TCTTTTTCCC
TCTGCCAAAA
ATTATGGGGA
CATCATGAAG
CCCCTTGAGC
ATCTGACTTC
TGGCTAATAA
AGGAAATTTA
TTTTCATTGC
AATAGTGTGT
TGGAATTTTT
TGTGTCTCTC
ACTCGGAAGG
ACATATGGGA
GGGCAAATCA
GGATCCGCGG
CCGCAAGGAT
CTGCGATCGC
TCCGGTGCCC
GTCAGTGGGC
AGAGCGCACA
TCGCCCACAG
TCCCCGAGAA
GTTGGGGGGA
GGGGTCGGCA
ATTGAACGGG
TGCCTAGAGA
AGGTGGCGCG
GGGTAAACTG
GGAAAGTGAT
GTCGTGTACT
GGCTCCGCCT
TTTTCCCGAG
GGTGGGGGAG
AACCGTATAT
AAGTGCAGTA
GTCGCCGTGA
ACGTTCTTTT
TCGCAACGGG
TTTGCCGCCA
GAACACAGCT
GAAGCTTCGA
GGGGCTCGCA
TCTCTCCTTC
ACGCGCCCGC
CGCCCTACCT
GAGGCCGCCA
TCCACGCCGG
TTGAGTCGCG
TTCTGCCGCC
TCCCGCCTGT
GGTGCCTCCT
GAACTGCGTC
CGCCGTCTAG
GTAAGTTTAA
AGCTCAGGTC
GAGACCGGGC
CTTTGTCCGG
CGCTCCCTTG
GAGCCTACCT
AGACTCAGCC
GGCTCTCCAC
GCTTTGCCTG
ACCCTGCTTG
CTCAACTCTA
CGTCTTTGTT
TCGTTTTCTG
TTCTGCGCCG
TTACAGATCC
AAGCTGTGAC
CGGCGCCTAC
GCTAGACGCC
ACCATGGAGA
GCGACGAGAG
CGGCCTGCCC
GCCATGGAGA
TCGAGTGCCG
CATCACCGGC
ACCCTGAACG
GCGTGGAGTT
CGAGCTGGTG
GGCGGCGGAG
AGGGCACCCC
CAAGCAGGGC
CGCATGACCA
ACAAGATGAA
GAGCACCAAA
GGCGCCCTGA
CCTTCAGCCC
CTACCTGCTG
AGCCACGTGA
TGGGCTACGG
CTTCTACCAC
TTCGGCACCT
ACCCCAGCGG
CTACGAGAAC
CCCTTCCTGC
ACGCCATCAA
CAACGGCGGC
TACACCAACA
CCCGCATCGA
GAAGTACGAG
GACGGCGGCG
TGCTGCACGT
GAGCTTCAGC
TACCGCTACG
AGGCCGGCCG
CGTGATCGGC
GACTTCAAGG
TGGTGGGCAC
CGGCTTCCCC
GAGGACAGCG
TGATCTTCAC
CGACAAGATC
ATCCGCAGCA
ACGCCACCGT
GGAGCACCTG
CACCCCATGG
GCGATAACGT
GCTGGTGGGC
AGCTTCGCCC
GCACCTTCAG
CCTGCGCGAC
GGCGGCTACT
ACAGCTTCGT
GGTGGACAGC
CACATGCACT
TCAAGAGCGC
CATCCACCCC
AGCATCCTGC
AGAACGGGGG
CCCCATGTTC
GCCTTCCGCC
GCGTGGAGGA
GCTGCACAGC
AACACCGAGC
TGGGCATCGT
GGAGTACCAG
CACGCCTTCA
AGACCCCCAT
CGCCTTCGCC
AGATCCCGCG
CTCAGTCGTC
CAATTCTGCC
GTGGACGGCA
CCGCCGGACC
CGGCTCCACC
GGATCTCGCG
AGGGCAGAGG
AAGTCTTCTA
ACATGCGGTG
ACGTGGAGGA
GAATCCCGGC
CCTATGACCG
AGTACAAGCC
CACGGTGCGC
CTCGCCACCC
GCGACGACGT
CCCCAGGGCC
GTACGCACCC
TCGCCGCCGC
GTTCGCCGAC
TACCCCGCCA
CGCGCCACAC
CGTCGATCCG
GACCGCCACA
TCGAGCGGGT
CACCGAGCTG
CAAGAACTCT
TCCTCACGCG
CGTCGGGCTC
GACATCGGCA
AGGTGTGGGT
CGCGGACGAC
GGCGCCGCGG
TGGCGGTCTG
GACCACGCCG
GAGAGCGTCG
AAGCGGGGGC
GGTGTTCGCC
GAGATCGGCC
CGCGCATGGC
CGAGTTGAGC
GGTTCCCGGC
TGGCCGCGCA
GCAACAGATG
GAAGGCCTCC
TGGCGCCGCA
CCGGCCCAAG
GAGCCCGCGT
GGTTCCTGGC
CACCGTCGGC
GTCTCGCCCG
ACCACCAGGG
CAAGGGTCTG
GGCAGCGCCG
TCGTGCTCCC
CGGAGTGGAG
GCGGCCGAGC
GCGCCGGGGT
GCCCGCCTTC
CTGGAGACCT
CCGCGCCCCG
CAACCTCCCC
TTCTACGAGC
GGCTCGGCTT
CACCGTCACC
GCCGACGTCG
AGGTGCCCGA
AGGACCGCGC
ACCTGGTGCA
TGACCCGCAA
GCCCGGTGCC
TGAAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCAGTT
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GGAATTGACT
CAAATGATGT
CAATTAGTCT
ATCAGAAGCT
ATCTGGTCTC
CCTTCCGGGG
GACAAGACAT
CCCTGTTTAA
TATTTAAACA
GCAGTGTTCC
CAAACTGGGT
TCTTATATCC
CTTGCTCTGG
TCAACCAGGT
TGCAGGGTTT
CCTGTCCTCA
CAGGAACGAA
GTCCCTAAAG
AAACAGTGGC
AGCCAGGTTT
AGCCCCGGAA
TTGACTGGAT
TCCTTTTTTA
GGGCCCATTG
GTATGGCTTT
TTCCCCGTAT
CCCCCCAGGT
GTCTGCAGGC
TCAAAGAGCA
GCGAGAAGCG
TTCAGAGGAA
AGCGATCCCG
TGCCACCTTC
CCCGTGCCCG
GGCTGTCCCC
GCACGCTGCC
GGCTCGGGGA
TGCGGGGGGA
GCGCCGGACC
GGAGCGGAGC
CCCGGGCGGC
TCGCTGCTGC
CCCCTAGCGG
GGGAGGGACG
TAATTACATC
CCTGGGGGCT
TTGGGGGGGG
GCTGTCCCTG
ATATCTATAA
CAAGAAAATA
TATATATAAT
AAGTTATCAC
GTAAGTAGAA
CATGAAATAA
CAATATAATT
ATCGTATGAG
TTAAATCTTA
AAAGTCACGT
AAAAGATAAT
CATGCGTCAT
TTTGACTCAC
GCGGTCGTTA
TAGTTCAAAA
TCAGTGACAC
TTACCGCATT
GACAAGCACG
CCTCACGGGA
GCTCCAAGCG
GCGACTGAGA
TGTCCTAAAT
GCACAGCGAC
GGATTCGCGC
TATTTAGAAA
GAGAGAGCAA
TATTTCAAGA
ATGCATGCGT
CAATTTTACG
CAGACTATCT
TTCTAGGGTT
AATCTAGCTG
CATCAGGATC
ATATCGTCGG
GTCTTTTTTC
CGGCTCAGTC
ATCGCCCAAG
CTGGCGCTAT
CTGGGCATCG
GGGAGGAAGA
AGCCCGTGCC
TTTTCCCGCG
AGGTTGAAGC
GGCATGGAAA
GAGTTTGCCG
AGGATGACTG
CTGCTGCATT
GACGTTGAGC
GAAAACGCAC
GTTTACCATG
ATGATTCGGG
AAGGTGTGGC
CATGCACGCC
TTTAACGGTG
AACTGTTCGT
TCAGGCCACC
TGGGATACCA
GTTCGTCGCG
GCTTTTCCGG
ACACAGTTCC
GGATGGTCAG
CCCGAAGCGC
ATCAGCAACC
CGAACAATAC
CGGCGACAGC
CGGAACTGCC
GTGCCGGTGT
GCAGATTAAT
GACAGCGGTG
CGGCGCTGGG
ATATTACGTC
AGCGAGGACG
GGTATCCTGG
CTGGATGCCG
CAGAAATGGA
CATGGATACC
CCGTGAGTTA
CCCGGCGGGC
GCGCTTGGCG
TAATCATGGT
CATAGCTGTT
TCCTGTGTGA
AATTGTTATC
CGCTCACAAT
TCCACACAAC
ATACGAGCCG
GAAGCATAAA
GTGTAAAGCC
TGGGGTGCCT
AATGAGTGAG
CTAACTCACA
TTAATTGCGT
TGCGCTCACT
GCCCGCTTTC
CAGTCGGGAA
ACCTGTCGTG
CCAGCTGCAT
TAATGAATCG
GCCAACGCGC
GGGGAGAGGC
GGTTTGCGTA
TTGGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GGACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TAATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT