名称: | pLV3-CMV-CTTN(pig)-6×His-Puro |
---|---|
别称: | XM_021082650.1; |
基因长: | 1629bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 猪 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-MCS-6×His-Puro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-CTTN(pig)-6×His-Puro是适用于哺乳细胞过表达的质粒。质粒携带的CMV强启动子可启动下游猪源CTTN基因转录,促进CTTN基因C端融合表达6×His标签,便于融合蛋白与镍离子发生亲和作用进行蛋白纯化,也可使用抗His抗体来检测和识别目的蛋白,有助于蛋白质的分离、纯化和检测等。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带Puro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATACTA
GTATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTTTA
TATAAGCAGA
GCTCGTTTAG
TGAACCGTCA
GATCGCCTGG
AGACGCCATC
CACGCTGTTT
TGACCTCCAT
AGAAGATTCT
AGAGCTAGCG
AATTCGCCAC
CATGTGGAAG
GCTTCGGCGG
GCCACACCGT
GTCCATCGCC
CAGGACGACG
GGGGAGCCGA
CGACTGGGAG
ACCGACCCCG
ACTTCGTGAA
TGACGTGAGT
GAGAAAGAGC
AGAGATGGGG
GGCCAAGACC
GTGCAAGGGT
CCGGGCACCA
GGAGCACATC
AACATACACA
AGCTGAGAGA
GAACGTGTTT
CAAGAGCACC
AGACCCTCAA
GGAGAAGGAG
CTCGAGACGG
GACCCAGAGC
CTCGCACGGC
TACGGAGGGA
AGTTCGGCGT
CGAGCAGGAC
AGGATGGACA
AATCGGCCGT
GGGCCACGAG
TACCAGTCGA
AGCTTTCCAA
GCACTGCTCG
CAGGTCGACT
CCGTCCGGGG
CTTTGGAGGC
AAGTTTGGCG
TCCAGGTGGA
CCGCGTTGAC
CAGTCGGCCG
TGGGCTTTGA
GTACCAGGGG
AAAACTGAGA
AGCACGCCTC
ACAGAAAGAT
TACGCGAGTG
GTTTTGGCGG
CAAGTACGGC
GTGCAGTCCG
ATCGTGTGGA
CAAGAGCGCG
GTGGGCTTCG
ACTACCAGGG
CAAGACGGAG
AAGCACGAGT
CGCAGAAAGA
CTACTCCAAA
GGCTTCGGCG
GCAAGTACGG
GATTGACAAG
GATAAAGTGG
ACAGGAGTGC
CGTGGGCTTT
GAGTATCAAG
GCAAAACGGA
GAAGCACGAG
TCCCAGAAAG
ACTACGTGAA
GGGCTTCGGA
GGGAAGTTTG
GCGTGCAGAC
AGACCGACAG
GACAAGTGTG
CCCTCGGCTG
GGACCACCAG
GAGAAGCTGC
AGCTGCACGA
GTCCCAGAAA
GATTATAAGA
CTGGTTTCGG
AGGCAGATTT
GGTGTTCAGT
CCGAGAGGCA
GGACTCCTGT
GCTGTGGGGT
TTGATTACAA
GGAGAAACTG
GCCAAGCACG
AGTCCCAGCA
AGATTATTCC
AGAGGATTCG
GCGGCAAGTA
CGGGGTGCAG
AAAGACCGGA
TGGATAAGAA
CGCCTCCACT
TTTGAGGACG
TGGCCCAGGT
GGCCCCCACC
TATCAGAAGA
CTGTCCCCGT
GGAAGCAGTG
AACAGCAAAA
CGAGCAACAT
CAGAGCCAAC
TTTGAAAACC
TGGCCAAGGA
GAAGGAGCAG
GAGGACCGGC
GGAAGGCGGA
GGCGGAGCGG
GCGCAGAGGA
TGGCGAAGGA
GAGGCAGGAG
CAGGAAGAAG
CCCGGCGGCA
GCTGGAGGAG
CAGGCCCGGG
CCCAGAAGCC
CACGCCGCCC
GCGTCCCCCA
CGCCTCAGCC
CGCTCCGGAG
AGGCCGCCGT
CGAGCCCCGT
CTACGAGGAC
GCGGCTCCCC
TCCAGGCCGA
GCCGAGCTAC
AGGGCCCCCG
AGAGCACACA
CGAGCCTGAG
CCGGTGTACA
GCGTGGAGGC
TGCCGGCTAC
CGAGAGGCCT
GCGCGCCGGA
GGCCGTCTAC
GAGAGCACGG
AGGCCCCGGG
CCACTACCCA
GCAGAGGAAG
GTGCCTATGA
CGAGTACGAG
AACGACCTTG
GAATCACGGC
CATCGCCCTG
TACGACTACC
AGGCTGCGGG
CGACGACGAG
ATCTCCTTCG
ACCCCGACGA
CATCATCACC
AACATCGAGA
TGATCGACGA
CGGCTGGTGG
CGCGGGCTCT
GCAAGGGCAG
GTACGGGCTC
TTCCCCGCCA
ACTACGTGGA
GCTGCGGCAG
GGATCCCATC
ACCATCACCA
TCACTAGCGG
CCGCGAAGGA
TCTGCGATCG
CTCCGGTGCC
CGTCAGTGGG
CAGAGCGCAC
ATCGCCCACA
GTCCCCGAGA
AGTTGGGGGG
AGGGGTCGGC
AATTGAACGG
GTGCCTAGAG
AAGGTGGCGC
GGGGTAAACT
GGGAAAGTGA
TGTCGTGTAC
TGGCTCCGCC
TTTTTCCCGA
GGGTGGGGGA
GAACCGTATA
TAAGTGCAGT
AGTCGCCGTG
AACGTTCTTT
TTCGCAACGG
GTTTGCCGCC
AGAACACAGC
TGAAGCTTCG
AGGGGCTCGC
ATCTCTCCTT
CACGCGCCCG
CCGCCCTACC
TGAGGCCGCC
ATCCACGCCG
GTTGAGTCGC
GTTCTGCCGC
CTCCCGCCTG
TGGTGCCTCC
TGAACTGCGT
CCGCCGTCTA
GGTAAGTTTA
AAGCTCAGGT
CGAGACCGGG
CCTTTGTCCG
GCGCTCCCTT
GGAGCCTACC
TAGACTCAGC
CGGCTCTCCA
CGCTTTGCCT
GACCCTGCTT
GCTCAACTCT
ACGTCTTTGT
TTCGTTTTCT
GTTCTGCGCC
GTTACAGATC
CAAGCTGTGA
CCGGCGCCTA
CGCTAGATGA
CCGAGTACAA
GCCCACGGTG
CGCCTCGCCA
CCCGCGACGA
CGTCCCCCGG
GCCGTACGCA
CCCTCGCCGC
CGCGTTCGCC
GACTACCCCG
CCACGCGCCA
CACCGTCGAC
CCGGACCGCC
ACATCGAGCG
GGTCACCGAG
CTGCAAGAAC
TCTTCCTCAC
GCGCGTCGGG
CTCGACATCG
GCAAGGTGTG
GGTCGCGGAC
GACGGCGCCG
CGGTGGCGGT
CTGGACCACG
CCGGAGAGCG
TCGAAGCGGG
GGCGGTGTTC
GCCGAGATCG
GCCCGCGCAT
GGCCGAGTTG
AGCGGTTCCC
GGCTGGCCGC
GCAGCAACAG
ATGGAAGGCC
TCCTGGCGCC
GCACCGGCCC
AAGGAGCCCG
CGTGGTTCCT
GGCCACCGTC
GGCGTCTCGC
CCGACCACCA
GGGCAAGGGT
CTGGGCAGCG
CCGTCGTGCT
CCCCGGAGTG
GAGGCGGCCG
AGCGCGCCGG
GGTGCCCGCC
TTCCTGGAGA
CCTCCGCGCC
CCGCAACCTC
CCCTTCTACG
AGCGGCTCGG
CTTCACCGTC
ACCGCCGACG
TCGAGGTGCC
CGAAGGACCG
CGCACCTGGT
GCATGACCCG
CAAGCCCGGT
GCCTGAGTCG
ACAATCAACC
TCTGGATTAC
AAAATTTGTG
AAAGATTGAC
TGGTATTCTT
AACTATGTTG
CTCCTTTTAC
GCTATGTGGA
TACGCTGCTT
TAATGCCTTT
GTATCATGCT
ATTGCTTCCC
GTATGGCTTT
CATTTTCTCC
TCCTTGTATA
AATCCTGGTT
GCTGTCTCTT
TATGAGGAGT
TGTGGCCCGT
TGTCAGGCAA
CGTGGCGTGG
TGTGCACTGT
GTTTGCTGAC
GCAACCCCCA
CTGGTTGGGG
CATTGCCACC
ACCTGTCAGC
TCCTTTCCGG
GACTTTCGCT
TTCCCCCTCC
CTATTGCCAC
GGCGGAACTC
ATCGCCGCCT
GCCTTGCCCG
CTGCTGGACA
GGGGCTCGGC
TGTTGGGCAC
TGACAATTCC
GTGGTGTTGT
CGGGGAAATC
ATCGTCCTTT
CCTTGGCTGC
TCGCCTGTGT
TGCCACCTGG
ATTCTGCGCG
GGACGTCCTT
CTGCTACGTC
CCTTCGGCCC
TCAATCCAGC
GGACCTTCCT
TCCCGCGGCC
TGCTGCCGGC
TCTGCGGCCT
CTTCCGCGTC
TTCGCCTTCG
CCCTCAGACG
AGTCGGATCT
CCCTTTGGGC
CGCCTCCCCG
CCTGGTACCT
TTAAGACCAA
TGACTTACAA
GGCAGCTGTA
GATCTTAGCC
ACTTTTTAAA
AGAAAAGGGG
GGACTGGAAG
GGCTAATTCA
CTCCCAACGA
AAATAAGATC
TGCTTTTTGC
TTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGT
AGTAGTTCAT
GTCATCTTAT
TATTCAGTAT
TTATAACTTG
CAAAGAAATG
AATATCAGAG
AGTGAGAGGA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
GCTCTAGCTA
TCCCGCCCCT
AACTCCGCCC
AGTTCCGCCC
ATTCTCCGCC
CCATGGCTGA
CTAATTTTTT
TTATTTATGC
AGAGGCCGAG
GCCGCCTCGG
CCTCTGAGCT
ATTCCAGAAG
TAGTGAGGAG
GCTTTTTTGG
AGGCCTAGAC
TTTTGCAGAG
ACGGCCCAAA
TTCGTAATCA
TGGTCATAGC
TGTTTCCTGT
GTGAAATTGT
TATCCGCTCA
CAATTCCACA
CAACATACGA
GCCGGAAGCA
TAAAGTGTAA
AGCCTGGGGT
GCCTAATGAG
TGAGCTAACT
CACATTAATT
GCGTTGCGCT
CACTGCCCGC
TTTCCAGTCG
GGAAACCTGT
CGTGCCAGCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGGACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATCT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTAAATTAA
AAATGAAGTT
TTAAATCAAT
CTAAAGTATA
TATGAGTAAA
CTTGGTCTGA
CAGTTACCAA
TGCTTAATCA
GTGAGGCACC
TATCTCAGCG
ATCTGTCTAT
TTCGTTCATC
CATAGTTGCC
TGACTCCCCG
TCGTGTAGAT
AACTACGATA
CGGGAGGGCT
TACCATCTGG
CCCCAGTGCT
GCAATGATAC
CGCGAGACCC
ACGCTCACCG
GCTCCAGATT
TATCAGCAAT
AAACCAGCCA
GCCGGAAGGG
CCGAGCGCAG
AAGTGGTCCT
GCAACTTTAT
CCGCCTCCAT
CCAGTCTATT
AATTGTTGCC
GGGAAGCTAG
AGTAAGTAGT
TCGCCAGTTA
ATAGTTTGCG
CAACGTTGTT
GCCATTGCTA
CAGGCATCGT
GGTGTCACGC
TCGTCGTTTG
GTATGGCTTC
ATTCAGCTCC
GGTTCCCAAC
GATCAAGGCG
AGTTACATGA
TCCCCCATGT
TGTGCAAAAA
AGCGGTTAGC
TCCTTCGGTC
CTCCGATCGT
TGTCAGAAGT
AAGTTGGCCG
CAGTGTTATC
ACTCATGGTT
ATGGCAGCAC
TGCATAATTC
TCTTACTGTC
ATGCCATCCG
TAAGATGCTT
TTCTGTGACT
GGTGAGTACT
CAACCAAGTC
ATTCTGAGAA
TAGTGTATGC
GGCGACCGAG
TTGCTCTTGC
CCGGCGTCAA
TACGGGATAA
TACCGCGCCA
CATAGCAGAA
CTTTAAAAGT
GCTCATCATT
GGAAAACGTT
CTTCGGGGCG
AAAACTCTCA
AGGATCTTAC
CGCTGTTGAG
ATCCAGTTCG
ATGTAACCCA
CTCGTGCACC
CAACTGATCT
TCAGCATCTT
TTACTTTCAC
CAGCGTTTCT
GGGTGAGCAA
AAACAGGAAG
GCAAAATGCC
GCAAAAAAGG
GAATAAGGGC
GACACGGAAA
TGTTGAATAC
TCATACTCTT
CCTTTTTCAA
TATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
GGTTCCGCGC
ACATTTCCCC
GAAAAGTGCC
ACCTGACGTC
TAAGAAACCA
TTATTATCAT
GACATTAACC
TATAAAAATA
GGCGTATCAC
GAGGCCCTTT
CGTCTCGCGC
GTTTCGGTGA
TGACGGTGAA
AACCTCTGAC
ACATGCAGCT
CCCGGAGACG
GTCACAGCTT
GTCTGTAAGC
GGATGCCGGG
AGCAGACAAG
CCCGTCAGGG
CGCGTCAGCG
GGTGTTGGCG
GGTGTCGGGG
CTGGCTTAAC
TATGCGGCAT
CAGAGCAGAT
TGTACTGAGA
GTGCACCATA
TGCGGTGTGA
AATACCGCAC
AGATGCGTAA
GGAGAAAATA
CCGCATCAGG
CGCCATTCGC
CATTCAGGCT
GCGCAACTGT
TGGGAAGGGC
GATCGGTGCG
GGCCTCTTCG
CTATTACGCC
AGCTGGCGAA
AGGGGGATGT
GCTGCAAGGC
GATTAAGTTG
GGTAACGCCA
GGGTTTTCCC
AGTCACGACG
TTGTAAAACG
ACGGCCAGTG
CCAAGCTG