名称: | pLV3-CMV-KRT18(human)-3×Myc-EF1-Hygro |
---|---|
别称: | NM_000224.3;CK-18; CYK18; K18 |
基因长: | 1290bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Hygro |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pCDH-CMV-MCS-EF1-Hygro |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
pLV3-CMV-KRT18(human)-3×Myc-EF1-Hygro是适用于哺乳细胞过表达的质粒。质粒带有的CMV强启动子可启动下游人源的KRT18基因转录,促进KRT18基因C端融合表达3×Myc标签,便于融合蛋白与镍离子发生亲和作用进行蛋白纯化,也可使用抗Myc抗体来检测和识别目的蛋白。此外,质粒带有HIV-1 Ψ和3'/5'LTR元件可用于慢病毒包装,便于基因稳定整合至宿主细胞。质粒携带Hygro抗性基因,用于筛选表达目的基因的宿主细胞。
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATACTA
GTATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTTTA
TATAAGCAGA
GCTCGTTTAG
TGAACCGTCA
GATCGCCTGG
AGACGCCATC
CACGCTGTTT
TGACCTCCAT
AGAAGATTCT
AGAGCCACCA
TGAGCTTCAC
CACTCGCTCC
ACCTTCTCCA
CCAACTACCG
GTCCCTGGGC
TCTGTCCAGG
CGCCCAGCTA
CGGCGCCCGG
CCGGTCAGCA
GCGCGGCCAG
CGTCTATGCA
GGCGCTGGGG
GCTCTGGTTC
CCGGATCTCC
GTGTCCCGCT
CCACCAGCTT
CAGGGGCGGC
ATGGGGTCCG
GGGGCCTGGC
CACCGGGATA
GCCGGGGGTC
TGGCAGGAAT
GGGAGGCATC
CAGAACGAGA
AGGAGACCAT
GCAAAGCCTG
AACGACCGCC
TGGCCTCTTA
CCTGGACAGA
GTGAGGAGCC
TGGAGACCGA
GAACCGGAGG
CTGGAGAGCA
AAATCCGGGA
GCACTTGGAG
AAGAAGGGAC
CCCAGGTCAG
AGACTGGAGC
CATTACTTCA
AGATCATCGA
GGACCTGAGG
GCTCAGATCT
TCGCAAATAC
TGTGGACAAT
GCCCGCATCG
TTCTGCAGAT
TGACAATGCC
CGTCTTGCTG
CTGATGACTT
TAGAGTCAAG
TATGAGACAG
AGCTGGCCAT
GCGCCAGTCT
GTGGAGAACG
ACATCCATGG
GCTCCGCAAG
GTCATTGATG
ACACCAATAT
CACACGACTG
CAGCTGGAGA
CAGAGATCGA
GGCTCTCAAG
GAGGAGCTGC
TCTTCATGAA
GAAGAACCAC
GAAGAGGAAG
TAAAAGGCCT
ACAAGCCCAG
ATTGCCAGCT
CTGGGTTGAC
CGTGGAGGTA
GATGCCCCCA
AATCTCAGGA
CCTCGCCAAG
ATCATGGCAG
ACATCCGGGC
CCAATATGAC
GAGCTGGCTC
GGAAGAACCG
AGAGGAGCTA
GACAAGTACT
GGTCTCAGCA
GATTGAGGAG
AGCACCACAG
TGGTCACCAC
ACAGTCTGCT
GAGGTTGGAG
CTGCTGAGAC
GACGCTCACA
GAGCTGAGAC
GTACAGTCCA
GTCCTTGGAG
ATCGACCTGG
ACTCCATGAG
AAATCTGAAG
GCCAGCTTGG
AGAACAGCCT
GAGGGAGGTG
GAGGCCCGCT
ACGCCCTACA
GATGGAGCAG
CTCAACGGGA
TCCTGCTGCA
CCTTGAGTCA
GAGCTGGCAC
AGACCCGGGC
AGAGGGACAG
CGCCAGGCCC
AGGAGTATGA
GGCCCTGCTG
AACATCAAGG
TCAAGCTGGA
GGCTGAGATC
GCCACCTACC
GCCGCCTGCT
GGAAGATGGC
GAGGACTTTA
ATCTTGGTGA
TGCCTTGGAC
AGCAGCAACT
CCATGCAAAC
CATCCAAAAG
ACCACCACCC
GCCGGATAGT
GGATGGCAAA
GTGGTGTCTG
AGACCAATGA
CACCAAAGTT
CTGAGGCATG
GATCCGAACA
AAAACTAATA
TCAGAGGAAG
ATTTGGAACA
GAAACTAATC
AGTGAAGAAG
ATTTAGAGCA
AAAGTTAATT
TCCGAGGAGG
ACTTATAGGG
ATCCGCGGCC
GCAAGGATCT
GCGATCGCTC
CGGTGCCCGT
CAGTGGGCAG
AGCGCACATC
GCCCACAGTC
CCCGAGAAGT
TGGGGGGAGG
GGTCGGCAAT
TGAACGGGTG
CCTAGAGAAG
GTGGCGCGGG
GTAAACTGGG
AAAGTGATGT
CGTGTACTGG
CTCCGCCTTT
TTCCCGAGGG
TGGGGGAGAA
CCGTATATAA
GTGCAGTAGT
CGCCGTGAAC
GTTCTTTTTC
GCAACGGGTT
TGCCGCCAGA
ACACAGCTGA
AGCTTCGAGG
GGCTCGCATC
TCTCCTTCAC
GCGCCCGCCG
CCCTACCTGA
GGCCGCCATC
CACGCCGGTT
GAGTCGCGTT
CTGCCGCCTC
CCGCCTGTGG
TGCCTCCTGA
ACTGCGTCCG
CCGTCTAGGT
AAGTTTAAAG
CTCAGGTCGA
GACCGGGCCT
TTGTCCGGCG
CTCCCTTGGA
GCCTACCTAG
ACTCAGCCGG
CTCTCCACGC
TTTGCCTGAC
CCTGCTTGCT
CAACTCTACG
TCTTTGTTTC
GTTTTCTGTT
CTGCGCCGTT
ACAGATCCAA
GCTGTGACCG
GCGCCTACGT
CGACATGAAA
AAGCCTGAAC
TCACCGCGAC
GTCTGTCGAG
AAGTTTCTGA
TCGAAAAGTT
CGACAGCGTC
TCCGACCTGA
TGCAGCTCTC
GGAGGGCGAA
GAATCTCGTG
CTTTCAGCTT
CGATGTAGGA
GGGCGTGGAT
ATGTCCTGCG
GGTAAATAGC
TGCGCCGATG
GTTTCTACAA
AGATCGTTAT
GTTTATCGGC
ACTTTGCATC
GGCCGCGCTC
CCGATTCCGG
AAGTGCTTGA
CATTGGGGAA
TTCAGCGAGA
GCCTGACCTA
TTGCATCTCC
CGCCGTGCAC
AGGGTGTCAC
GTTGCAAGAC
CTGCCTGAAA
CCGAACTGCC
CGCTGTTCTG
CAGCCGGTCG
CGGAGGCCAT
GGATGCGATC
GCTGCGGCCG
ATCTTAGCCA
GACGAGCGGG
TTCGGCCCAT
TCGGACCGCA
AGGAATCGGT
CAATACACTA
CATGGCGTGA
TTTCATATGC
GCGATTGCTG
ATCCCCATGT
GTATCACTGG
CAAACTGTGA
TGGACGACAC
CGTCAGTGCG
TCCGTCGCGC
AGGCTCTCGA
TGAGCTGATG
CTTTGGGCCG
AGGACTGCCC
CGAAGTCCGG
CACCTCGTGC
ACGCGGATTT
CGGCTCCAAC
AATGTCCTGA
CGGACAATGG
CCGCATAACA
GCGGTCATTG
ACTGGAGCGA
GGCGATGTTC
GGGGATTCCC
AATACGAGGT
CGCCAACATC
TTCTTCTGGA
GGCCGTGGTT
GGCTTGTATG
GAGCAGCAGA
CGCGCTACTT
CGAGCGGAGG
CATCCGGAGC
TTGCAGGATC
GCCGCGGCTC
CGGGCGTATA
TGCTCCGCAT
TGGTCTTGAC
CAACTCTATC
AGAGCTTGGT
TGACGGCAAT
TTCGATGATG
CAGCTTGGGC
GCAGGGTCGA
TGCGACGCAA
TCGTCCGATC
CGGAGCCGGG
ACTGTCGGGC
GTACACAAAT
CGCCCGCAGA
AGCGCGGCCG
TCTGGACCGA
TGGCTGTGTA
GAAGTACTCG
CCGATAGTGG
AAACCGACGC
CCCAGCACTC
GTCCGAGGGC
AAAGGAATAG
GTCGACAATC
AACCTCTGGA
TTACAAAATT
TGTGAAAGAT
TGACTGGTAT
TCTTAACTAT
GTTGCTCCTT
TTACGCTATG
TGGATACGCT
GCTTTAATGC
CTTTGTATCA
TGCTATTGCT
TCCCGTATGG
CTTTCATTTT
CTCCTCCTTG
TATAAATCCT
GGTTGCTGTC
TCTTTATGAG
GAGTTGTGGC
CCGTTGTCAG
GCAACGTGGC
GTGGTGTGCA
CTGTGTTTGC
TGACGCAACC
CCCACTGGTT
GGGGCATTGC
CACCACCTGT
CAGCTCCTTT
CCGGGACTTT
CGCTTTCCCC
CTCCCTATTG
CCACGGCGGA
ACTCATCGCC
GCCTGCCTTG
CCCGCTGCTG
GACAGGGGCT
CGGCTGTTGG
GCACTGACAA
TTCCGTGGTG
TTGTCGGGGA
AATCATCGTC
CTTTCCTTGG
CTGCTCGCCT
GTGTTGCCAC
CTGGATTCTG
CGCGGGACGT
CCTTCTGCTA
CGTCCCTTCG
GCCCTCAATC
CAGCGGACCT
TCCTTCCCGC
GGCCTGCTGC
CGGCTCTGCG
GCCTCTTCCG
CGTCTTCGCC
TTCGCCCTCA
GACGAGTCGG
ATCTCCCTTT
GGGCCGCCTC
CCCGCCTGGT
ACCTTTAAGA
CCAATGACTT
ACAAGGCAGC
TGTAGATCTT
AGCCACTTTT
TAAAAGAAAA
GGGGGGACTG
GAAGGGCTAA
TTCACTCCCA
ACGAAAATAA
GATCTGCTTT
TTGCTTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGTAGTAGT
TCATGTCATC
TTATTATTCA
GTATTTATAA
CTTGCAAAGA
AATGAATATC
AGAGAGTGAG
AGGAACTTGT
TTATTGCAGC
TTATAATGGT
TACAAATAAA
GCAATAGCAT
CACAAATTTC
ACAAATAAAG
CATTTTTTTC
ACTGCATTCT
AGTTGTGGTT
TGTCCAAACT
CATCAATGTA
TCTTATCATG
TCTGGCTCTA
GCTATCCCGC
CCCTAACTCC
GCCCAGTTCC
GCCCATTCTC
CGCCCCATGG
CTGACTAATT
TTTTTTATTT
ATGCAGAGGC
CGAGGCCGCC
TCGGCCTCTG
AGCTATTCCA
GAAGTAGTGA
GGAGGCTTTT
TTGGAGGCCT
AGACTTTTGC
AGAGACGGCC
CAAATTCGTA
ATCATGGTCA
TAGCTGTTTC
CTGTGTGAAA
TTGTTATCCG
CTCACAATTC
CACACAACAT
ACGAGCCGGA
AGCATAAAGT
GTAAAGCCTG
GGGTGCCTAA
TGAGTGAGCT
AACTCACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCATAGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGG
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
CGTCTAAGAA
ACCATTATTA
TCATGACATT
AACCTATAAA
AATAGGCGTA
TCACGAGGCC
CTTTCGTCTC
GCGCGTTTCG
GTGATGACGG
TGAAAACCTC
TGACACATGC
AGCTCCCGGA
GACGGTCACA
GCTTGTCTGT
AAGCGGATGC
CGGGAGCAGA
CAAGCCCGTC
AGGGCGCGTC
AGCGGGTGTT
GGCGGGTGTC
GGGGCTGGCT
TAACTATGCG
GCATCAGAGC
AGATTGTACT
GAGAGTGCAC
CATATGCGGT
GTGAAATACC
GCACAGATGC
GTAAGGAGAA
AATACCGCAT
CAGGCGCCAT
TCGCCATTCA
GGCTGCGCAA
CTGTTGGGAA
GGGCGATCGG
TGCGGGCCTC
TTCGCTATTA
CGCCAGCTGG
CGAAAGGGGG
ATGTGCTGCA
AGGCGATTAA
GTTGGGTAAC
GCCAGGGTTT
TCCCAGTCAC
GACGTTGTAA
AACGACGGCC
AGTGCCAAGC
TG