引物名称

引物序列

pQE-R

GTTCTGAGGTCATTACTGG

Psiren-shuttle.F

TCTTGGGTAGTTTGCAGTTT

WPRE-R

CATAGCGTAAAAGGAGCAACA

Psicheck-2-R

CCCCCGCGAGGTCCGAAGAC

T3

ATTAACCCTCACTAAAGGGA

T72G

TAATACGACTCACTATAGG

T7(17base)

ACATCCACTTTGCCTTTCTC

PVL1393.R

GGGTCCAAGTTTCCCTGTAG

SP6

ATTTAGGTGACACTATAGAA

SV40pro-F

TATTTATGCAGAGGCCGAGG

PSG5-F

CCATGTTCATGCCTTCTTCT

pTriEX-1.R

ATTTGTGAGCCAGGGCATTG

U6

ATGGACTATCATATGCTTACCGTA

pTRCHISC.F

AAGGAGGAATAAACCATGGG

psiCHECK-2-F

GAAGTTCCCTAACACCGAGT

pQE30-F

TGAGCGGATAACAATTTCAC

pTRC99C-F

TTGCGCCGACATCATAAC

Reverse

gtaacatcagagattttgagacac

pRetro-On.F

TTTAGTGAACCGTCAGATCG

pTarget.R

GGCTTTACACTTTATGCTTC

pSG5-R

GTTTGGACAAACCACAACTA

pTRCHISC.R

CTGTATCAGGCTGAAAATCT

pTRC99C-R

CTGCGTTCTGATTTAATCTG

pShuttle-F

GAAGTGAAATCTGAATAATTTTGT

Sv40-polyA-R

GAAATTTGTGATGCTATTGC

pTRE2.R

CCCCCTCCATATAACATGAA

S1

CAACGTGAAAAAATTATTATTCGC

RVP3

CTAGCAAAATAGGCTGTCCC

Reverse(right ITR)

TACTATGGTTGCTTTGACGT

RVP4

GACGATAGTCATGCCCCGCG

pTarget.F

CCAGGATTTTCCCAGTCAC

PYES.F

CCTTTTCGGTTAGAGCGGAT

pTRE2.F

TCGTTTAGTGAACCGTCAGA

pShuttleCMV.R

GTGGTATGGCTGATTATGATCAG

S6

GTAAATGAATTTTCTGTATGAGG

pmscv-3

ACGTGCTACTTCCATTTGTC

T7ter

TGCTAGTTATTGCTCAGCGG

T7

TAATACGACTCACTATAGGG

PYES2.R

TCGGTTAGAGCGGATGTG

S.tag

CGAACGCCAGCACATGGACA

pMEP4-F

CTAGCTATAAACACTGCTTG

PLCNX-R

ACCTACAGGTGGGGTCTTTCATTCCC

PLKO.1-R

CTGCACTGTACCCCCCAATC

pGEX-3'

CCGGGAGCTGCATGTGTCAGAGG

pEGFP-C-5'

CATGGTCCTGCTGGAGTTCGTG

pEGFP-N-5’

TGGGAGGTCTATATAAGCAGAG

PFBT.F

TATTCCGGATTATTCATACC

PLEXA-N.R

TAAAACCTAAGAGTCACTTT

PFAST-F

TATTCCGGATTATTCATACC

PGBT9-F

GGCTTCAGTGGAGACTGATA

PIRES-F

CTTACTGACATCCACTTTGC

PGAP-F

GTCCCTATTTCAATCAATTGAA

Petblue-2.f

TGCGGTAGTTTATCACAG

PM.F

CATAGAATAAGTGCGACATC

Petblue-2.f

GATTCTCCCAGTCACGAC

pDSRED-N1-R

TGAAGCGCATGAACTCCTTG

pECFP-C-R

TCATTTTATGTTTCAGGTTCAG

pIRES2-EGFP.P5'

GTAGGCGTGTACGGTGGGAG

pINPOINTPrimer

CGTGACGCGGTGCAGGGCG

PLXSN-F

CTTGAACCTCCTCGTTCGAC

PGBT9-R

ACAGGAAAGAGTTACTCAAG

pFlag-CMV-R

GCACTGGAGTGGCAACTT

pFastBac-R

CCTCTACAAATGTGGTATGG

PLEXA-N.F

CGTCAGCAGAGCTTCACCAT

pEGFP-N-3'

CGTCGCCGTCCAGCTCGACCAG

pEF-F

TCAAGCCTCAGACAGTGGTTC

PM.R

TTTATGTTTCAGGTTCAGGG

pGEX-5'

GGGCTGGCAAGCCACGTTTGGTG

pJET1.2-F

CGACTCACTATAGGGAGAGCGGC

PH

AAATGATAACCATCTCGC

PFASTBAC-2R

GACCTTTAATTCAACCCAAC

pIRES2-EGFP.P3'

AACGCACACCGGCCTTATTC

PIRES.R

CACTGCATTCTAGTTGTGGT

pEGFP-C-3'

TATGGCTGATTATGATCAGT

pJET1.2-R

AAGAACATCGATTTTCCATGGCAG

pMEP4-R

GCAGCTTATAATGGTTACAAAT

pFLAG-CMV-F

GGTAGGCGTGTACGGTGG

pB42ADF

CCAGCCTCTTGCTGAGTGGAGATG

pCMV-R

TCCAAACTCATCAATGTATC

PBR322.R

GCCTGCCACCATACCCAC

pDSRED2-N-R

CGTTCACGGTGCCCTCCATG

PDB.F

GAATAAGTGCGACATCATCATC

Pcmv5r

ATTATAGAAGGACACCTAGTC

PBR322.F

AAGTGCCACCTGACGTCTAA

pBI121-R

GTTTTCGCGATCCAGACTGA

pBABE-3

accctaactgacacacattcc

pcaggs-R

TATGTCCTTCCGAGTGAGAG

pDsRED-express-C1-F

TCCCACAACGAGGACTACAC

pCold-R

GGCAGGGATCTTAGATTCTG

pB42ADR

AAGCCGACAACCTTGATTGGAG

PBAD30.R

CACTTCTGAGTTCGGCATGG

PDC316.F

ACGTGGGTATAAGAGGCG

pBV220R

CTGCGTTCTGATTTAATCTG

pBABE-5

ctttatccagccctcac

pBADfw

CCATAGCATTTTTATCCATAAG

pCold-F

ACGCCATATCGCCGAAAGG

Pcmv5f

TTCCAAAATGTCGTAATAAC

PBAD30.F

AGATTAGCGGATCCTACCTG

pBV220F

AAGAAGGGCAGCATTCAAAG

PDC316.R

CGATGCTAGACGATCCAG

pCMV-F

TCTAAAAGCTGCGGAATTGT

pAS2-1.R

AAGAGTTACTCAAGAACAAGAA

PDB.R

GTAAATTTCTGGCAAGGTAGAC

pcDNA3.1F

CTAGAGAACCCACTGCTTAC

pCEP-F

AGAGCTCGTTTAGTGAACCG

pcDNA3.1R

TAGAAGGCACAGTCGAGG

pcaggs-F

GTTCGGCTTCTGGCGTGT

H1

TCGCTATGTGTTCTGGGAAA

HSVtk-pA-R

GTCTCCTTCCGTGTTTCAG

NEBF

CCCGCCGCTGCTTTTGCACGTGAG

P3'

CTGCAAGGCGATTAAGTTGG

P20

AAGAGTTACTCAAGAACAAGAA

GFPfusionREV

CTCCACTGACAGAAAATTTG

M13F(-47)

CGCCAGGGTTTTCCCAGTCACGAC

hGH-PA-R

CCAGCTTGGTTCCCAATAGA

P1

CCAGGCTTTACACTTTATGC

NOS

GATAATCATCGCAAGACCGG

GFP-R

CCATCTAATTCAACAAGAATTGGGACAAC

M13R

CAGGAAACAGCTATGACC

PABAI-R

ATGCTACAAAGGACCTAATG

P5'

TGCGTACTGCGGTGATCAAC

PAAV-MCS-F

TCATACCTCTTATCTTCCTC

P2

GCGATTAAGTTGGGTAACGC

GLP4

GGGTAGAATGGCGCTGGG

P17110

TACCACTACAATGGATG

NEBR?

TATGCTAGTTATTGCTCAG

pACT2.R

GTGCACGATGCACAGTTGAA

MalE primer

GGTCGTCAGACTGTCGATGAAGCC

GLP2

CTTTATGTTTTTGGCGTCTTCC

GFP-F

GGTCCTTCTTGAGTTTGTAAC

PAO-AAM.F

GACTGGTTCCAATTGACAAG

Forward(left ITR)

CCTCTGACTTGAGCGTCGAT

GTGCACGATGCACAGTTGAA

M13F（M13-20）

TGTAAAACGACGGCCAGT

GLP1

TGTATCTTATGGTACTGTAACTG

mU6F

CAGCACAAAAGGAAACTCACC

MCHERRY-F

AACGAGGACTACACCATCGT

mCherry-R

TTGGTCACCTTCAGCTTGG

ITS4

TCCTCCGCTTATTGATATGC

M13R(-48)(即RV-M 引物）

AGCGGATAACAATTTCACACAGGA

P24990

TCATCGGAAGAGAGTAG

ITS1

TCCGTAGGTGAACCTGCGG

P10

GCCTCTAACATTGAGACAGC

3'BD

TAAGAGTCACTTTAAAATTTGTATAC

BAC1

AACCATCTCGCAAATAAATA

Forward

TCGcgttaacgctagcatggatctc

BAC2

ACGCACAGAATCTAGCGCTT

1492R

TACGGCTACCTTGTTACGACTT

BGH

TAGAAGGCACAGTCGAGG

35S

GACGCACAATCCCACTATCC

DUETUP2

TTGTACACGGCCGCATAATC

27F

AGAGTTTGATCCTGGCTCAG

3'AD

AGATGGTGCACGATGCACAG

CMV-30

ATAACCCCGCCCCGTTG

3'AOX(pPIC9K-R)

GGCAAATGGCATTCTGACAT

CMV-F

CGCAAATGGGCGGTAGGCGTG

3Xflag.R

TAATTTTATTAGGACAAGGCTG

EBV-R

GTGGTTTGTCCAAACTCATC

CMV-24

TTAGGACAAGGCTGGTGG

5'AOX(pPIC9K-F)

GACTGGTTCCAATTGACAAGC

a-Factor

TACTATTGCCAGCATTGCTGC

DUETUP1

GATCTCGACGCTCTCCCT

DuetDOWN1

GATTATGCGGCCGTGTACAA

3Xflag.F

GCTCTTGTTGGAGCTGCAGT