	   SnapGene      rGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCAGTGTGGTGGAATTCGCCACCatggtgaggagcaggcaaatgtgcaataccaacatgtctgtacctactgatggtgctgtaaccacctcacagattccagcttcggaacaagagaccctggttagaccaaagccattgcttttgaagttattaaagtctgttggtgcacaaaaagacacttatactatgaaagaggttcttttttatcttggccagtatattatgactaaacgattatatgatgagaagcaacaacatattgtatattgttcaaatgatcttctaggagatttgtttggcgtgccaagcttctctgtgaaagagcacaggaaaatatataccatgatctacaggaacttggtagtagtcaatcagcaggaatcatcggactcaggtacatctgtgagtgagaacaggtgtcaccttgaaggtgggagtgatcaaaaggaccttgtacaagagcttcaggaagagaaaccttcatcttcacatttggtttctagaccatctacctcatctagaaggagagcaattagtgagacagaagaaaattcagatgaattatctggtgaacgacaaagaaaacgccacaaatctgatagtatttccctttcctttgatgaaagcctggctctgtgtgtaataagggagatatgttgtgaaagaagcagtagcagtgaatctacagggacgccatcgaatccggatcttgatgctggtgtaagtgaacattcaggtgattggttggatcaggattcagtttcagatcagtttagtgtagaatttgaagttgaatctctcgactcagaagattatagccttagtgaagaaggacaagaactctcagatgaagatgatgaggtatatcaagttactgtgtatcaggcaggggagagtgatacagattcatttgaagaagatcctgaaatttccttagctgactattggaaatgcacttcatgcaatgaaatgaatcccccccttccatcacattgcaacagatgttgggcccttcgtgagaattggcttcctgaagataaagggaaagataaaggggaaatctctgagaaagccaaactggaaaactcaacacaagctgaagagggctttgatgttcctgattgtaaaaaaactatagtgaatgattccagagagtcatgtgttgaggaaaatgatgataaaattacacaagcttcacaatcacaagaaagtgaagactattctcagccatcaacttctagtagcattatttatagcagccaagaagatgtgaaagagtttgaaagggaagaaacccaagacaaagaagagagtgtggaatctagtttgccccttaatgccattgaaccttgtgtgatttgtcaaggtcgacctaaaaatggttgcattgtccatggcaaaacaggacatcttatggcctgctttacatgtgcaaagaagctaaagaaaaggaataagccctgcccagtatgtagacaaccaattcaaatgattgtgctaacttatttccccCTCGAGTACCCATACGACGTACCTGATTATGCAACTTATCCTTATGATGTACCTGATTATGCTAGCTATCCTTATGATGTCCCAGACTACGCATAGTGAGGGCCCGATATCTCGACAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGGAAACGGGGGAGGCTAACTGAAACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCACGGGTGTTGGGTCGTTTGTTCATAAACGCGGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATACCCCACCGAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCGGGGCGGCAGGCCCTGCCATAGCAGATCTGCGCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGGGGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGG  @      #            *   !      "            #      -                   1   +               #   $               !   "   "            !      $         #                        !   '   "         -      )   -      (      +      !      #      %               !         %   #      !   ,      $   /   C   &      &                  !         *                     &      "         '   -                     #      
                                  (   )      +   +   #   (                  (   &                                  #   #         
      '   $   "   "         '      %      .   ,   "      $   1               %   %   !      &      "             !   $            !                                 &   3   )         $      '   #                %               %   &   !   (                      .   )      (      "            0   %      %      (      -         )       2         Y $	d

]^V{)S
@|#  x b\2[%


_be5? Z  77iVQAT  N	e
r
FDW|*|TN 9} t  c]3&	`vs6* %^:V?

WrE\'UCQn! l	S	
::npI f@g  y!.j\(
_


Zfc%"  _NKYy	
8jWx$Rcy  MR5/	^sBX- #n		8p\J{BwU [ fIIBm#  OV	<
XU?
W>!Wp6`R4~ ] 		s
[+Mt5H-  {!	f
k

cE}h+v
-F1BUMrOjQ:o2M     T~3	
 G>_}HN.I|F dl8	aFLqtV7 D4'*w )s_ u  @^),	5lU%& tw^}8ZRc11 ;VD<?hH
s;xiDt)Ga+ h@H_r;bQ#	Xk]"p(F^Vo~ &RfwW8FV&sd F@
RW  s Am65o % 6-DT	|/q'0WAh# 7 3#~	'	
>d#;+1Jguv$ bmB;nPr!,4L^    AJE]	

2dG 1 Z)n	`
y
y&g!"V >  z"/Wk+	
/C;&'   p(k
Ukoe[	,`a!R!  `ZQ				
zCZ%GUwzO" >B`>P+f!	9koz\" #YL')z3		

JLX?SZ" OA	DyOdMkb+ SJkwl]$( M   N2+|AtC!   %	g0	j	~		{&"_Y.w  3j[6q$ . K;5>					o


oK h'#   C ~ $>Rd|`WxV  	[S9)# c w  o3	9qA]M[nCu /
7S)J&  \   DN.	
NJf
Jn7 gG	C\ 


atx P=



Y=@X}U\qaue  6nXT]S/  , | W	m?75!  
sNV{"CQJ-' q"GM!J\kuquCP69O
T" Hm C		8Rm3I ^	,FxJ] Qd	t
N-   vTY			Y
\
l


x ,.G2CNkP@RO# |KL	q	
d`V_s4f>)  5yS8\	w		%
FwL2P:,i;3Nj- v"A	
g

F~i1k$4EeGSJhp"=|*d
 I
HIO9</J:gf( !  ./`v
7

Me~! /<L   Y4d	L
P

%KSRX=|}+ j    7Ue=]b	U

+?`$X{]G0  1Z#c
C6hURd8S! $i~9'U	ur e$H em4JX IW#  U	b=^gsGMu	o5_QGL skv()+E7Qk% EC>aGPwh!(V}c $ 52A&	m*.+t&u 'Yjm1x%j!` "A_O*eCynvNLa* L   2iIfzk%'! B b v =	2QmV& F-6` 
Mm~lz[Std% p!FLjp8N	~SI2# uxSRJ

w_39OdF<{= i .u\L;! Wh0Tt<HncP
FK, XjuB=@`iFO%	i- '*b !1<E~d1_yj$u`$ o-EQI[
lEsH</ ,DWigAcENS$	^#Gm,~r_	C *UVf<G
"R	[\]+,)W|}opq^p5&  P+JI`s=Hz		$-*_ 6 	#Yl^qXq&    GQ,Kaot >{a	G:+T t I   -X|	v:D'> S JIu	|
.9=3`q7Y O 'SgxZ		
%)WCe   	ZP3SIr 4G$m("r{EC!  
7|8	A
g_	Y*X q		 r   RvL1	
n)/",G0*  Hnfpu!?B|	DpqU\a_   -91?i<	O	H[f!u ,ctbkb	o	}A76; J g   hjMQ   m.EY	*(B & n p *k7}?m ( a.U	}
H$~w0r1X;E(.i& 4 ;   >B2[(	t	
4
 a	kDYZ 8 F T _ `AJ	e:KvA"  8K		?$/4=,h wLoy8 	4$ov2	}

>ca<2r8
 +cF
&*uz@X
,' P '(c[y		l	,b$Day{MhP- y:Tn, 	<o"5Bf hCy	
.(Qsg-	_  W  K]:
[pKy>3=  3'%T.J    B	


Qu^s *  5F^J	W		4JeHf  	HX0%a
#"Fi# 5SsOX^o		z
z) 'oyADW 2  l8*=sh|ZOk
 [Mnw# ,			v
hD82? ? cXl9	0'+   {#]f}
`(Y 80B

<iZ*0  l\
7&mZl"GS )r
fi2? q Ql	



PpY-t  	%*Vvab  wMa[	q
Ez{ PM  s  ]	R	^

VD[~&9mBPm( "Mx.				
o#HAxl,)   S\z} 2			
*0	-HVq=4{E1 ?C7	]prb%+ :gU?Q;Gqvg"	j:E]s%Fr`+ 6 a r,
"@C}cl"54O{q3V]# 0 =     @IoDV*		x

.:FQ( =&(=2@ 	P				
BIKYFTY[j  $Zp*{ji		!\#v  J:4=		n


@RcZg" .M		

MIes	w[  
r<BTP, PclB	~B7EQl\(  4u7@	[	p	v			[


E0eN)c	i& -dac	
6
O
T

dW8z\ e/    T5gT^m &l6%| A K a  Bxm) h ~KRik2NMzR n/}Zb;   OCF	M	#+] H N  ,`	<2BuC   37	f'2D) q  +bue80h;	N			|@CZ/ ' o jl`
	#)# : E  =@Zn1	s	3
<b~@CXnx# &xk	'af`~	+xzO  ) V  4\I	V	
JIx=-2<  k7rW/L<	hYj@
 b)/ ~Xu%1ls>YF' L|\	p
D }pL<k+l$ / 5 <   ?C9Tq: 6)	bNr2$EqP" 
o<	h				
d biZ" 
 3?	u			
5
S


(.[  ` %Q4AfSLy{l G B6	_	&1;a\:' 9 D at?_w/	;?e}*wyBNW U   K;	H	Ik=X% 	 	89	g		C|c 1j*p v:G	^		3@H%0:>x[Mz9.  7stuF		56789yz{ab4\8i89,  }%L		
!
'

5-]T:9:"  "

"(4
b.RNy d {5hUw	r=59;$ pp3		

?^6@o:v1 x   ~&
	M%y	
(
~



:dB3^Us(.;\D4 ,i


85T'	+SGOz d	Sc{A/* 0YKM $'  #
G

#)Hv 2>-!% 2|s	
/OKcbL|:Q% Q   Edzx
	
gk
%zN8<! ] e ()6i/m		D
- >
EKo6fi,_ OV\s%m6g\j& xH;$	

p@U[37DCg hU!A0;" qzo-4		 _&p6,w2  q			= U#] y :i

;!CuR_h)]y ?  '&

e)4wt
r! DNC				



tV/W.~5$  
zz	)
/


?N /bk<IE`  X 


\6U(a~	?4>{ -k>

9qYTof  LQ^[wH]rzbAv! ;
j



ZL
,E>ATLiPVn3 4K|(YU^T$0 e7oU  =0/K  	TndB+ _& U X		
> yN!  1}	v@  - } 	nZL8_(t   C


R(%6  + { 

5|\I. G	ItOw&  K	X	$
X
K1^j#+3?DRhIg!"3     6X<_H
K

$*JR#Q;W9<f{.K) 
#3I}Y"&	b
B]frQd#4GR # r1(0lvDg$PjtU hHNePMK$  t

KvoLyc7:};  0tP_0hk&OEJ' )I+Te!Dy	Z*l{n4# R    F nYt	{

{(9pS9=X  6{p			O!zH B mj0>n	
(.!Fp[e`^ f m   )gGiL}P-l  3 ^   _A17	 
`7	jK% *9bx+E
-+ty?#Ag 9
ot&$]e RNW]nvxN \e		u
7h1> P
	

$)Oh6k%  [y!w	-			]

Uy8OlA3@D&  FU		w

i!-\4~39E$! I<%		
m

AHrE QXYv(  6`Z	N
Z



cqV/D[ASdPh @ }15 Le"1 <idYm:	M	r	2
a`B( V
Wtg<,?  {Snp	R
aM;q-O  s^P.5	j)<k.  |$6r9E	]	f			
 
&

B2Gx`3~07Z7)  zgT~x	



!'?Q'-4x3 	h

GR1=FL#( 1rC

.	l5fj	$K[ie+^ w#		 TZ2/%T+1" 9B			
.
>Mj vHVq>} j
	DZvSK`hnm@2 <Jm^#T |		
=x'0 z    W;J
W

IQ"P:0]8;i*gz[H% " g'd
Aenfx3Js   /(*s/NYjG% l    @0 aw
f'*_"@d 	

8

#(g5u0=dM(  Z E5_	
M


Tbq}ZW@Xcu ;1
~- rRmOD	e		
Q





&A1>}/6 		-

-LQSYu.0K   V{		V

P Yw1) k    f?c	"'.@Xdm&^IcFc  :^,
0


,@a0|?Jb2   89>{|}~		*
+
O!&+,-Wcl%=>Hab  /  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                         ,                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                         	                                                                                                                                                                                   *   	                                                                               &                  	                                                             
                  	                              1      
      	            	      	               
                           
                                 	            0         #   !      $                j                     d                                                                                                                                                                    ,                                                                                                                                                                                                                                                                                                                       
         	                                                                 * 	                           &      	                     
      	          1  
  	    	  	     
         
           	    0   # !  $      j       d   |	  })  }G  }      k	tk 		V


T./N"=]xg~u]:8o5q  ULd\  .Ud	L
P

\uIOG=C\
j`
a eRi>mBiOj$Yi^8?)@
_i  A

k")irYMUxiZcr}-HI	-6z  A]

Ud4* =? tO	bO	F :|O	r
y}te
D|&LUh&S	pgafcIVgU!m8MhUxFoBvq	$_t8



@Ad'J?ium ?m8R1 
Jgl	q	mxNH 
'  tO	e
r
D|ybpo_="
'%1 U|4L
U

F\qJgX/8



'=lA:m + E
tM
[<
W:!ytzvq o7Z
). )Q. YOk	q") |ioD&		eg7c %p%or_X* ]7JgW


vIh
v)	$>k	!RG  Pipz"ql: BPr,^ Z 	
TAe3@	\w_ Y	Y

Ju.C@R69O  Tj	

F>_|ZF
Y`18jOjj
ZJu69 Y	Y

.C@ROaw*"@m:
K< Y	I
Y


HJYuO.9=@C/:@RUf69O Z 	

TAeY`3@1,xs65,		2?gdJn-1r.K\$ x [

b
a K]	D&y1Pr.\K$kNMK		u
71>E]	dG5  VV

P 
}&/Rf3w#,
5;1V;dR(%,/8;[3S]D



&>Vs;~  2NCW<?h)a &w2,5/;N&Rf3w#
;1VdI

HYO9=@/:UfN		u
71>MKfpuD_d 5h	=5p
 + { 

5|\I.z    W;J
W

IQ"P:0]8;i*gz[HP=



Y=@X}U\qaue 3j[6quxSRJ

w_39OdF<{= 
sNV{"CQJ-@ }15bmB;nPr!,4L^   T~3	
 G>_}HN.I|Fj
	DZvSK`hnm@2mj0>n	
(.!Fp[e`^  i   Tu{.~x!SRu3J\		
f
k



 Gc>EL_w}}h+HN_v
-F1BU.IMrOj39OQdF;|:Fo2<{=M' f m o q    )+bmujl`e8
j00>h;	N	n				
g|(@.G!iC#FL})Zp/[eP-`l^   B	


Qu^sPclB	~B7EQl\S JIu	|
.9=3`q7Y		-

-LQSYu.0Kdl8	aFLqtV7G B6	_	&1;a\:   Bqeu m4JQXlxSR J		








QwPu_pYI-39OdFtW^<{s=d	Sc{A/*  .               )3Fg2hi䦖Imi-f2	X?>?\-e&+Is@zXjpvԉcԜ-aVσiv4ɤic
v d¡Khr**}VX]{5`0Ȝ M92zSoy/Hԡ[lA+-*aUIJ.aR5іV^g%PmtRtuQaUY6tҸ{i"(i RM5#J6b6EWџ`]EQ}ꖨU>xejv\mgGzI4aعiPMha ZZ8UIHIr~c^'99bsdn[_uZThx&^b9:>mh`ڮ[࣋A(C\ "5
 =Ӿίh>>8 87Z;>"~/9[<;>̺Ϭİn<9xWpPGYP_5Qe\,@hs*ԬPO\4?dѸ8 &s?0ͥ3
s4|z=33=V6
#~Uz@
ܒ?G
?r/rHWuꆦP>h)Ua8j]Usb"Ґ"KM66i-5"KM66i-5K^+?8yj>9E$S:&ojnQl6QsB΀uÎ<4?
|~/08=+8(0hR<A۷Rom50g:ßS6ܞOaQ6x;Pp`>:<Y`klxÀ0|vLξp<ƳL>ĮL?_mjƽϮaP:lac+^4
ueMdujYSj6({mbnbg9m8!Z)ֽvq<R%O#億Z2Wial=s0C
;:;x{8Ì10N%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3BnҰ+;8_+O3%ˡ/_QzlM=A1	#9EI&;TJ	;5u}L FUE sqW)@jFUAߑZUaJmnTI^ҟXm!Ln.j({$e@HXgG;|;ѩW@3E 5o>NDҖJ(9 sʂ`kNPħa(UL);(RR"{`7cF:!ԶE&0ģ?Y=4^R;mU ^A|oJ%xn۪z*!5WACU5UeXg{hSjm.L٨XUՐ
7jH`ud=&㿎,$jSuC֍       !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  $0<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1572" maxFusedRunOn="1572" detectionMode="exactProteinMatch"><Segment range="1543-1569" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1572" maxFusedRunOn="1572" detectionMode="exactProteinMatch"><Segment range="1513-1539" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1572" maxFusedRunOn="1572" detectionMode="exactProteinMatch"><Segment range="1483-1509" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4499-4515" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4475-4491" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4568-4589" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4523-4529" color="#ffffff" type="standard"/><Segment range="4530-4547" color="#ffffff" type="standard"/><Segment name="-35" range="4548-4553" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2247-2295" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6497-6601" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4305-4426" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3119-3254" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2939-3268" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2497-2925" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4877-5465" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1593-2181" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3335-4129" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (Geneticin®)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6427" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5636-6427" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6428-6496" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="DCUN1D1" directionality="1" translationMW="30125.05" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1572" maxFusedRunOn="1572"><Segment range="700-1476" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>133751de-e061-46cb-a9a0-62e6e87ef9e0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="7:18:9">2022.11.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   <StrandColors><TopStrand><ColorRange range="1016..1016" colors="magenta"/></TopStrand><BottomStrand><ColorRange range="1016..1016" colors="magenta"/></BottomStrand></StrandColors>  /               )3Fg2hi䦖Imi-f2	X?>?\-e&+Is@zXjpvԉcԜ-aVσiv4ɤic
v d¡Khr**}VX]{5`0Ȝ M92zSoy/H]JRҦnHU]4V)]jofq"UAE)N'YFeZgM+a7=6"2+D]1+]ai!dUyPFZqAJjQoAPiX=zH`QZV(m}Tw[aFaE>+գuTtğ'5龘b~c/6H@fuUFk(sc&掿&ﾉ
8?(52*8s_~-;p{lÊxu ,N`(kr㜿?0~0øˬKϿρ\ CwuaYUUʊ\+,g=g?2J;4 CMӀ;o3S0 3Lw??;ήܬ-Nlo@:7W` =dp(+dx]je j悕VZ1ik"-) TIScn֒_B TIScn֒_d??o{Ҽ>RHAU;faʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J{+1X|}LsFk<
ʯL8詣N`VTMVՕ6k*/W&.&|:-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:X$&+N|>^l%Ǧx4	tM9+H yÝPtn3OJ~UDWW8$
M4iUPnQp;H{r-iTꕥVVI.)ߛ欭"F^qn5|sýMe}=<4R^oKZ(II+=)dr=;,&
qJvbTE%(<)
`xkx=cI}KkY"c|J;Ye3ݓEU#\U(Fb_׆M}2Ut5XUPP1{gY6)Y]Y 3vT~'[6JSn;舠BHMV9P:hQn:      !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  $<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="19" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1578" maxFusedRunOn="1578" detectionMode="exactProteinMatch"><Segment range="1549-1575" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="18" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1578" maxFusedRunOn="1578" detectionMode="exactProteinMatch"><Segment range="1519-1545" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1578" maxFusedRunOn="1578" detectionMode="exactProteinMatch"><Segment range="1489-1515" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4505-4521" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4481-4497" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4574-4595" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4529-4535" color="#ffffff" type="standard"/><Segment range="4536-4553" color="#ffffff" type="standard"/><Segment name="-35" range="4554-4559" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2253-2301" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6503-6607" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4311-4432" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3125-3260" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2945-3274" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2503-2931" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4883-5471" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1599-2187" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3341-4135" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (Geneticin®)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6433" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5642-6433" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6434-6502" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="DCUN1D1" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1578" maxFusedRunOn="1578"><Segment range="706-1482" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>133751de-e061-46cb-a9a0-62e6e87ef9e0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="7:18:0">2022.11.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="704..704" colors="green"/><ColorRange range="1022..1022" colors="magenta"/></TopStrand><BottomStrand><ColorRange range="704..704" colors="green"/><ColorRange range="1022..1022" colors="magenta"/></BottomStrand></StrandColors>  +                )3Fg2hi䦖Imi-f2	X?>?\-e&+Is@zXjpvԉcԜ-aVσiv4ɤic
v d¡Khr**}VX]{5`0Ȝ M92zSoy/H4f ğ'5龘b~c/6H@fuUFk(sc&掿&ﾉ
8?(52*8s_~-;p{lÊxu ,N`(kr㜿?0~0øˬKϿρ\ CwuaYUUʊ\+,g=g?2J;4 CMӀ;o3S0 3Lw??;ήܬ-Nlo@:7W` =dp(+dx]je j悕VZ1ik"-) TIScn֒_B TIScn֒_d??o{Ҽ>RHAU;faʳau74+<ka7]l8ȯ-3@3-Gaxq3lp2ӈrxS@x8#4-~&8cX6s9}1]lLM,mm OC͏A,cś`V:ǈ<8md!8Q:1`8|쎧	k4ͳ>J{+1X|}LsFk<
ʯL8詣N`VTMVՕ6k*/W&.&|:-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:X$&+N|>^l%Ǧx4	tM9+H yÝPtn3OJ~UDWW8$
M4iUPnQp;H{r-iTꕥVVI.)ߛ欭"F^qn5|sýMe}=<4R^oKZ(II+=)dr=;,&
qJvbTE%(<)
`xkx=cI}KkY"c|J;Ye3ݓEU#\U(Fb_׆M}2Ut5XUPP1{gY6)Y]Y 3vT~'[6JSn;舠BHMV9P:hQn:  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="18" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5677" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4886-5677" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5678-5746" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2585-3379" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (Geneticin®)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="843-1431" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4127-4715" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1747-2175" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2189-2518" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2369-2504" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3555-3676" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5747-5851" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1497-1545" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3773-3779" color="#ffffff" type="standard"/><Segment range="3780-3797" color="#ffffff" type="standard"/><Segment name="-35" range="3798-3803" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3818-3839" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3725-3741" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3749-3765" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="822" maxFusedRunOn="822" detectionMode="exactProteinMatch"><Segment range="733-759" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="822" maxFusedRunOn="822" detectionMode="exactProteinMatch"><Segment range="763-789" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="822" maxFusedRunOn="822" detectionMode="exactProteinMatch"><Segment range="793-819" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>133751de-e061-46cb-a9a0-62e6e87ef9e0</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="7:14:42">2022.11.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  5                 )3Fg2hi䦖Imi-f2	X?>?\-e&+Is@zXjpvԉcԜ-aVσiv4ɤic
v d¡Khr**}VX]{5`0Ȝ M92zSoy/H4f UyftWQU)j!V	bsc::&V(J  "76}z=Ϗ@;(ּ(8rψ߁3r.?q,>>p^|9eTtYW7(*+es /87 ʺ+?@,4n3N @	L3iL 29ޏLL:rë;jHp߱U^!$ªKܮ+]wn溡
UXNUj\ť XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5TNڬGA*͆`hЮ D99tuc"8 º?A49q3N#-L n;T`c8Άvͱ17'`S豴nM=<^+=36?11mX< "9(9hD0ŀ:&;6Ϯlq+ a1q|T([*<8o3 nJ?ע:eYSY6ZVTڪJ ^XظND2[`N*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2c8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR[[&fWt:[HC~nJ>,yp:}?8jm5P<LH/zϽ.ihS%$5(t6"철:Ӽ+1)o#US
N-H)MeQx!'-d1(-g|vMThr1WPn8ұ{#^6ꞭʸMUPcU@Y@pV(Yfڛ@vj&UeudڿR=rF,{n;<)OI"8I#15Z]CP|uaE   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ! <?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1316" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="933" maxFusedRunOn="933" detectionMode="exactProteinMatch"><Segment range="1317-1328" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3752-3768" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3728-3744" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3821-3842" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3776-3782" color="#ffffff" type="standard"/><Segment range="3783-3800" color="#ffffff" type="standard"/><Segment name="-35" range="3801-3806" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1500-1548" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5750-5854" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3558-3679" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2372-2507" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2192-2521" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1750-2178" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4130-4718" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="846-1434" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2588-3382" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (Geneticin®)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5680" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4889-5680" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5681-5749" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="3xMyc" directionality="1" translationMW="3573.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="825" maxFusedRunOn="825" detectionMode="exactProteinMatch"><Segment range="733-822" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;3 tandem Myc epitope tags&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="R1" sequence="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="731-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC" meltingTemperature="69"><Component hybridizedRange="731-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC"/></BindingSite><BindingSite location="761-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTTGTTCC" meltingTemperature="62"><Component hybridizedRange="773-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATT"/><Component bases="AGTTTCTGTTCCAAATCTTCCTCTGATATT"/><Component hybridizedRange="768-772" bases="AGTTT"/><Component bases="T"/><Component hybridizedRange="761-766" bases="TGTTCC"/></BindingSite><BindingSite simplified="1" location="731-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC" meltingTemperature="69"><Component hybridizedRange="731-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTCTGTTCCAAATCTTCCTCTGATATTAGTTTTTGTTCC"/></BindingSite><BindingSite simplified="1" location="761-828" boundStrand="1" annealedBases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATTAGTTTTGTTCC" meltingTemperature="62"><Component hybridizedRange="773-828" bases="CTCACTATAAGTCCTCCTCGGAAATTAACTTTTGCTCTAAATCTTCTTCACTGATT"/><Component bases="AGTTTCTGTTCCAAATCTTCCTCTGATATT"/><Component hybridizedRange="768-772" bases="AGTTT"/><Component bases="T"/><Component hybridizedRange="761-766" bases="TGTTCC"/></BindingSite></Primer><Primer recentID="0" name="F1" sequence="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="727-832" boundStrand="0" annealedBases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC" meltingTemperature="71"><Component hybridizedRange="727-832" bases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC"/></BindingSite><BindingSite simplified="1" location="727-832" boundStrand="0" annealedBases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC" meltingTemperature="71"><Component hybridizedRange="727-832" bases="TCGAGGAACAAAAACTAATATCAGAGGAAGATTTGGAACAGAAACTAATCAGTGAAGAAGATTTAGAGCAAAAGTTAATTTCCGAGGAGGACTTATAGTGAGGGCC"/></BindingSite></Primer></Primers>
  ><Notes>
<UUID>554b079e-04cd-46e5-910a-3c8d9ddfc533</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="8:15:15">2020.4.4</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  *T                 )3Fg2hi䦖Imi-f2	X?>?\-e&+Is@zXjpvԉcԜ-aVσiv4ɤic
v d¡Khr**}VX]{5`0Ȝ M92zSoy/H4f uGpQPaI!V	bsc::&V(J  "76}z=Ϗ@;(ּ(8rψ߁3r.?q,>>p^|9eTtYW7(*+es /87 ʺ+?@,4n3N @	L3iL 29ޏLL:rë;jHp߱U^!$ªKܮ+]wn溡
UXNUj\ť XRCCE&5MZKM}RCCE&5MZKM}??JZ4C<NkI!:>C5TNڬGA*͆`hЮ D99tuc"8 º?A49q3N#-L n;T`c8Άvͱ17'`S豴nM=<^+=36?11mX< "9(9hD0ŀ:&;6Ϯlq+ a1q|T([*<8o3 nJ?ע:eYSY6ZVTڪJ ^XظND2[`N*Vo;uw]O6TSa!9{v;x=8X[\£&ξ3L&ӷC	g
ot[@sAQv!5PC_%T<82Cq6UԴBWy2;#;+ϫ2c8"B8"7o	yKw=Ox%i4LBbCD wAj	=)UNo]_|**54ѧ3EUAQF. \UPPnoVXR[[&fWt:[HC~nJ>,yp:}?8jm5P<LH/zϽ.ihS%$5(t6"철:Ӽ+1)o#US
N-H)MeQx!'-d1(-g|vMThr1WPn8ұ{#^6ꞭʸMUPcU@Y@pV(Yfڛ@vj&UeudڿR=rF,{n;<)OI"8I#15Z]CP|uaE   !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !H<?xml version="1.0"?><Features nextValidID="18"><Feature recentID="17" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5656" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4865-5656" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5657-5725" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2564-3358" color="#ccffcc" type="standard" translated="1"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (Geneticin®)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="822-1410" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4106-4694" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1726-2154" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2168-2497" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2348-2483" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3534-3655" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5726-5830" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="798" consecutiveTranslationNumbering="1" maxRunOn="801" maxFusedRunOn="801" detectionMode="exactProteinMatch"><Segment range="733-798" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAG® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1476-1524" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3752-3758" color="#ffffff" type="standard"/><Segment range="3759-3776" color="#ffffff" type="standard"/><Segment name="-35" range="3777-3782" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3797-3818" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3704-3720" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3728-3744" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1292" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="909" maxFusedRunOn="909" detectionMode="exactProteinMatch"><Segment range="1293-1304" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  =<Notes>
<UUID>70bcc161-158a-4f5f-9630-d4c4e88666d6</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="8:9:43">2020.4.4</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   7zXZ  ִF !   t/] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!n;_xѱ*p$*5i@'Vl"{zIߨe<mZ:&
Wp+sx^rtҒrkeޣ>/A{S|HPT@paRhQib%jo;k~Ei\	oxQQ:<ӊQ vuS<}et˝'(I2޳ !`-^d]ː/\C#$upds1u5n+	~M7Ѡ!G+wTW6?5{&J'A<pñڂ!;dnsƛ Ǒ8Sp"8mF5Er>~OPӽU-0Vs#":ip7:OhY\U:ԧÓ웛QBi
n1Z}Y8I]!$ns>m -#:JW1}`7 &."FaU~͋1X?"e˗C>:׽$lp3.@p],L.g'#V*mWoq$1=Š+C'Uoa]#&0YKRx_ZM2ӣ*/ MUᝥ6$P^|l>Lv}FsFx'ɠ\W0vj?횾e㫶髌EWU I}'Mˣ>gag$65hq/jJ=QYmjʫiq:5aڐBYW'Br_@u-}dOa=t̜ʣؘXyT	.*G%4r}ճ'#M%|N!?Uf_ Aܮ%}5'֕P<'M;( K7ft3WP*%)C()~u`Ku
'WA¬tx#!}ۡ\]]Q<iԣkb^޿D|f	4k=MK~du[C0th=>q rb-3h#=הg*E36x  +Fcv6sÛla7
hm5[HݭfYEk|蛚vV؜q)dcQ$3TQlĶFqm1z.H_\:FEu46)O"o:G'"56<J{Lf">^Aa&gx2AB29^is_2,>onh>"Y>ZPu5Ds,c>cUO $m+2+"Ԛ!>WH~P Hj5oN2Hk/t#df,i/R0![n}ETPםv\VC[] ħc-qh@~[0z7.&JJTP%\\U%(~Jww/A݇,mռA'UqN'b/_	gLŒDH+<;F2rSĖ;vL6=ioF,
pg"KZԴ|T2 AiCɶcT||Fw1C<wHZ`J^40eo }Or'+FQ	oqkxMcWh蚾11a Kz$A݉@}fE	3hsyh+fT2ݙDi*[亇ǙB=$ZVi۟C .n}NH#we;箾b6_(KxdQ>ij@1Ɯ`Y`;"/=%׵SA:+y ,SjP WN`Y1Hk+%Pvf_=ڏ 5	X+Nҍ}W-H!R`!pRkFpp
Zat/B~WoBAzF>璝:jm5(CKDVr+xn*i5Y!D>BB\֍s3PP
EsѦJ]~xsnfPtw_598;ͤc+\=oX犡)9j5:ҹsؐlld3AJ4Ӊg^C'Q'5;u-/MD
C #Mc
x: Ͱf'?O'wsxt^U,JlwMXy!8jAԯ܌iv3,n5m~XM'%aN2JHEj@wXiK&IUί2x0<ޕ
}9Iq@`;S*VƮrB&_ f<cuxWPZsyb,;;ף_Wl.SHǒcL/Ɍu$*CμRvkABG9*b߄Y[NhSIJ짹N]À:b?J~	svw?5Dܓa~ˮ75|z^B?ڛ}e9T띣S٢Y6E~R2]ԈOEy[a+3S{.%Z5X*|d۪n%r*ZH-B>Hv{ZEiïѬmYګv\LWB tyfJSˬ 9]{&оd5~чђRj0^U[2CaЎ	k_5}-@cco47+)xZ쐦>_J)Q&<m-.1kJ~d5I=^@8IgfcΨ.h6ZG6=PSfAu=WCrF6(ø\7FF&6cήmPt!<
1<%	B@-
ҤvHi8EuP>Ԕ__zxq     wuc
Р  dg    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  +N<?xml version="1.0"?><Features nextValidID="21"><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7147" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6356-7147" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7148-7216" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="1" name="NeoR/KanR" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4055-4849" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (Geneticin®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="2" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2313-2901" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5597-6185" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3217-3645" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="50-429" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3659-3988" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="430-633" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3839-3974" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5025-5146" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7217-7321" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="HSV TK poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2967-3015" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;herpes simplex virus thymidine kinase polyadenylation signal&amp;nbsp;&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/3018551&quot;&gt;(Cole and Stacy, 1985)&lt;/a&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5243-5249" color="#ffffff" type="standard"/><Segment range="5250-5267" color="#ffffff" type="standard"/><Segment name="-35" range="5268-5273" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5288-5309" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5195-5211" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5219-5235" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2292" maxFusedRunOn="2292" detectionMode="exactProteinMatch"><Segment range="2203-2229" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="17" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2292" maxFusedRunOn="2292" detectionMode="exactProteinMatch"><Segment range="2233-2259" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="18" name="HA" directionality="1" translationMW="1102.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2292" maxFusedRunOn="2292" detectionMode="exactProteinMatch"><Segment range="2263-2289" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;HA (human influenza hemagglutinin) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="YPYDVPDYA"/></Q></Feature><Feature recentID="19" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="700-709" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="20" name="MDM2" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2292" maxFusedRunOn="2292"><Segment range="706-2196" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MVRSRQMCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  <Notes>
<UUID>3f91dab5-a904-44a6-846a-af33e6e171dd</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-MDM2(human)-3×HA-Neo</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="5:0:4">2019.1.23</Created>
<LastModified UTC="17:37:39">2025.9.18</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                        d      DO  d              E              Y? P                                                                                                                                                              <StrandColors><TopStrand><ColorRange range="0..7536" colors="black"/></TopStrand><BottomStrand><ColorRange range="0..7536" colors="black"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
